Evaluation of Single Radial Immunodiffusion for Quantitation of Plasma Fibrinogen

Gm, Brittin; Rafinia, H; Raval, Dhaval; Werner, Mario; Brown, Barry L.

doi:10.1093/ajcp/57.1.89

Cited by 22 publications

(7 citation statements)

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“…Quantitative radioimmunochemical studies were performed in a four compartment double antibody system as previously described (13 (23) using goat anti-fg antisera. This assay is generically similar to other reported radial immunodiffusion assays (24,25 (29). The primary diagnostic criterion for in vivo fibrinolysis was the presence of serum cleavage products of > 5.0 mg/100 ml.…”

Section: Introductionsupporting

confidence: 61%

Immunobiology of Fibrinogen. EMERGENCE OF NEOANTIGENIC EXPRESSIONS DURING PHYSIOLOGIC CLEAVAGE IN VITRO AND IN VIVO

Plow¹,

Edgington²

1973

J. Clin. Invest.

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A B S T R A C T Physiological degradationand E-fragments exhibit more than 10-fold greater native fibrinogen antigenic expression than the sum of the constituent fragments. This suggests the presence of a non-covalently associated native complex of the D-and E-fragments, and implies contiguity of the Dand E-fragments in the native fibrinogen molecule. The cleavage-associated neoantigen, fg-Dn.0, is also generated in vivo and is generically demonstrable in the plasma of patients with various forms of in vivo fibrinolysis.These studies offer a precise immunochemical system, based upon defined molecular events, for the investigation of physiological and pathophysiological cleavage of fibrinogen. By contrast with other approaches to the This work was presented in part at the American Society of Hematology Meetings, San Francisco, Calif., December 1971.

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Section: Introductionsupporting

confidence: 61%

Immunobiology of Fibrinogen. EMERGENCE OF NEOANTIGENIC EXPRESSIONS DURING PHYSIOLOGIC CLEAVAGE IN VITRO AND IN VIVO

Plow¹,

Edgington²

1973

J. Clin. Invest.

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“…This represents a significant decrease in response to iron deficiency, as has already been reported. 14) A 10 mg iron loading for 1 h in the control rats did not affect the Ft mRNA and protein expression ( Fig. 2A and C).…”

mentioning

confidence: 79%

Rapid Regulation of Intestinal Divalent Metal (Cation) Transporter 1 (DMT1/DCT1) and Ferritin mRNA Expression in Response to Excess Iron Loading in Iron-Deficient Rats

Arita

Tadai

Shinoda

2010

Bioscience, Biotechnology, and Biochemistry

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“…49 Values obtained were verified by immunonephelometry and radial immunodiffusion according to Mancini. 3,9,28 Fibrinogen determinations with the various methods must correlate closely (Table 3) to provide a good standard for FPA. Under these conditions, clotting releases all the FPA available.…”

Section: Calibrationmentioning

confidence: 99%

Development and Performance Characteristics of a Competitive Enzyme Immunoassay for Fibrinopeptide A

Amiral¹,

Walenga²,

Fareed³

1984

Semin Thromb Hemost

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A simple nonisotopic method for the quantitation of FPA in biologic samples has been developed utilizing a competitive enzyme immunoassay technique. The performance characteristics of these assays have been investigated in both the experimental and clinical settings and were found to be satisfactory for the routine clinical application. This method is comparable to a commercially available RIA kit (Mallinckrodt, St. Louis, MO) in both the clinical and normal samples. This assay can be used in the diagnosis of hypercoagulable states associated with various diseases. Subclinical activation of coagulation can be readily assessed when the global tests, such as the prothrombin time, partial thromboplastin time, and thrombin time, have no value. This test is of value in the monitoring of the newer antithrombotic agents, such as the low molecular weight heparin fractions that do not effect the global assays, such as the partial thromboplastin time. Similarly, the risk of thrombosis associated with the use of procoagulant therapy, such as the activated prothrombin complex concentrates, can be readily assessed using this assay. It is proposed that the FPA measurement may also provide useful information on the quality control of various plasma-based therapeutic products, such as plasma concentrates or activated prothrombin complex concentrates. FPA generation tests are currently proposed for the screening of antithrombotic and prohemostatic agents.

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Evaluation of Single Radial Immunodiffusion for Quantitation of Plasma Fibrinogen

Cited by 22 publications

References 0 publications

Immunobiology of Fibrinogen. EMERGENCE OF NEOANTIGENIC EXPRESSIONS DURING PHYSIOLOGIC CLEAVAGE IN VITRO AND IN VIVO

Immunobiology of Fibrinogen. EMERGENCE OF NEOANTIGENIC EXPRESSIONS DURING PHYSIOLOGIC CLEAVAGE IN VITRO AND IN VIVO

Rapid Regulation of Intestinal Divalent Metal (Cation) Transporter 1 (DMT1/DCT1) and Ferritin mRNA Expression in Response to Excess Iron Loading in Iron-Deficient Rats

Development and Performance Characteristics of a Competitive Enzyme Immunoassay for Fibrinopeptide A

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