Evaluation of some essential oils for the control and prevention of American Foulbrood disease in honey bees

Albo, Graciela Noemí; Henning, Cynthia; Ringuelet, Jorge Abel; Reynaldi, Francisco José; Giusti, Marisa Raquel De; Alippi, Adriana Mónica

doi:10.1051/apido:2003040

Cited by 43 publications

(36 citation statements)

References 15 publications

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“…The concentrations of the natural compounds in the candy were chosen on the basis of preliminary tests and previous research (Albo et al, 2003;Maistrello et al, 2003) and are shown in Table I. The candy, contained in a 3 cm diameter Petri dish (9 g), was positioned upside-down on a 1 cm diameter hole on top of the cage.…”

Section: Bee Susceptibility and Diet Preference Experimentsmentioning

confidence: 99%

Screening of natural compounds for the control of nosema disease in honeybees (Apis mellifera)

et al. 2008

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-The potential of some natural compounds (thymol, vetiver essential oil, lysozyme, resveratrol) for the control of nosema infection in honeybees was evaluated. A first trial aimed at screening substances, in candy preparations, on the basis of their toxicity to honeybees and bees' dietary preferences. None of the tested substances showed an increased bee mortality or decreased bee preference, and were therefore considered suitable for further testing. In the second trial the effects of the natural compounds on nosema diseased honeybees were evaluated: bees were individually dosed with nosema spores and fed candies prepared with the screened substances. The results showed that bees fed with thymol and resveratrol candies had significantly lower infection rates, and bees supplied with resveratrol prepared candy also lived significantly longer. We suggest that thymol and resveratrol could be useful in alternative strategies for the control of nosema disease.Nosema / microsporidian / honeybee / thymol / resveratrol

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Section: Bee Susceptibility and Diet Preference Experimentsmentioning

confidence: 99%

Screening of natural compounds for the control of nosema disease in honeybees (Apis mellifera)

et al. 2008

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“…Plant essential oils have been studied for their antimicrobial activity against microorganisms, including many pathogens (Dorman and Deans, 2000;Delaquis et al, 2002). In vitro antibacterial, antifungical and miticide activity of some essential oils have shown effective results in the control of bee pests (Calderone and Shimanuki, 1994;Alippi et al, 1996;Floris et al, 1996;Bazzoni and Floris, 1999;Albo et al, 2001Albo et al, , 2003Ruffinengo et al, 2002Ruffinengo et al, , 2005Ruffinengo et al, , 2006Dellacasa et al, 2003;Eguaras et al, 2005;Fuselli et al, 2006Fuselli et al, , 2007 offering a natural desirable alternative to antibiotics and other synthetic chemical substances.…”

Section: Introductionmentioning

confidence: 99%

In vitro antibacterial effect of exotic plants essential oils on the honeybee pathogen Paenibacillus larvae, causal agent of American foulbrood

Fuselli

Rosa

Eguaras

et al. 2010

Span. j. agric. res.

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Chemical composition and antimicrobial activity of exotic plants essential oils to potentially control Paenibacillus larvae, the causal agent of American foulbrood disease (AFB) were determined. AFB represents one of the main plagues that affect the colonies of honeybees Apis mellifera L. with high negative impact on beekeepers worldwide. Essential oils tested were niaouli (Melaleuca viridiflora) and tea tree (Melaleuca alternifolia) from Myrtaceae, and citronella grass (Cymbopogon nardus) and palmarosa (Cymbopogon martinii) from Gramineae. The components of the essential oils were identified by SPME-GC/MS analysis. The antimicrobial activity of the oils against P. larvae was determined by the broth microdilution method. In vitro assays of M. viridiflora and C. nardus oils showed the inhibition of the bacterial strains at the lowest concentrations tested, with minimal inhibitory concentration (MIC) mean value about 320 mg L -1 for both oils, respectively. This property could be attributed to the kind and percentage of the components of the oils. Terpinen-4-ol (29.09%), α-pinene (21.63%) and limonene (17.4%) were predominant in M. viridiflora, while limonene (24.74%), citronelal (24.61%) and geraniol (15.79%) were the bulk of C. nardus. The use of these essential oils contributes to the screening of alternative natural compounds to control AFB in the apiaries; toxicological risks and other undesirable effects would be avoided as resistance factors, developed by the indiscriminate use of antibiotics.Additional key words: antimicrobial activity, chemical composition, Cymbopogon martini, Cymbopogon nardus, Melaleuca alternifolia, Melaleuca viridiflora, honeybees' pathogen. ResumenActividad antibacteriana in vitro de los aceites esenciales de plantas exóticas frente al patógeno bacteriano Paenibacillus larvae, agente causal de loque americana en colonias de abejas melíferas Se determinó la composición química y se evaluó la actividad antimicrobiana in vitro de cuatro aceites esenciales de plantas exóticas para el control del patógeno bacteriano Paenibacillus larvae, agente causal de loque americana, que afecta a los estadios de larva y pupa de la abeja melífera (Apis mellifera L.). Esta enfermedad produce grandes pérdidas económicas en la apicultura mundial. Los aceites esenciales analizados, pertenecientes a la familia Myrtaceae y Gramineae, fueron: niaouli (Melaleuca viridiflora) y árbol del té (Melaleuca alternifolia), citronela (Cymbopogon nardus) y palmarosa (Cymbopogon martinii), respectivamente. La composición química de los aceites esenciales se efectuó por cromatografía de gases acoplada a un espectrómetro de masas (CG-EM). La concentración inhibitoria mínima (MIC) se determinó por el método de microdilución en caldo y la concentración bactericida mínima (MBC) en agar MYPGP. Los aceites esenciales de niaoulí y citronela registraron valores promedio de MIC de 320 mg L -1 , atribuidos al tipo y porcentaje de sus componentes: terpinen-4-ol (29,09%), α-pineno (21,63%) y limoneno (17,4%) que predominaron ...

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“…American foulbrood is considered a global threat to apiculture because the etiologic agent produces environmentally stable spores which are very virulent, and resistant to Antimicrobial activity and toxicity of B. thyrsoides heat, to desiccation, and to disinfectants (Genersch, 2010;Boligon et al, 2013a). Products derived from plants, such as propolis (Bastos et al, 2008;Mihai et al, 2012), essential oils (Albo et al, 2003;Santos et al, 2012); and extracts of Myrtus communis L., Eucalyptus dunnii (Maiden), Rosmarinus officinalis L., Zingiber officinale (Roscoe), Scutia buxifolia (Reissek), among other species (Flesar et al, 2010;Boligon et al, 2013a) were previously investigated and exhibited a growth-inhibitory effect against AFB. Buddleja is a genus with pan tropical distribution that occurs in South Asia, Africa, and America (Mahlke et al, 2009).…”

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confidence: 99%

Potential Use of Buddleja Thyrsoides for the Control and Prevention of American Foulbrood Disease in Honey Bees

Boligon

Piana

Brum

et al. 2014

Journal of Apicultural Science

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a b s t r a c t Paenibacillus larvae is the causative agent of american foulbrood (afb), a severe disease that affects the larvae of the honeybees. the use of plant extracts are considered to be an alternative way of controlling the disease. in this study, the in vitro antimicrobial activity of buddleja thyrsoides lam. against the Paenibacillus species, including P. larvae, was evaluated. in mueller-hinton broth, the minimal inhibitory concentration (miC) was assessed using the microdilution method. all Paenibacillus species were sensitive to the crude extract and the fractions of b. thyrsoides. the ethyl acetate (ea) fraction showed a better result with miC values of 1.68 -3.36 mg/ml, followed by butanolic (bU) (miC = 2.18 -6.54 mg/ml), dichloromethane (dCm) (7.40 -14.80 mg/ml), and crude extract (Ce) (7.51 -16.90 mg/ml). the toxic effect of the Ce and fractions of b. thyrsoides against bees were also evaluated using the spraying application method with the same concentrations of miCs. bee mortality was evident in treatment with dCm fractions only, while Ce, ea, and bU extracts showed no toxic effects after 15 days of observation. furthermore, phenolic acids, tannins, and flavonoids were identified and quantified by highperformance liquid chromatography (hPlC), and may be partially responsible for the antimicrobial properties observed. these results show, for the first time, that b. thyrsoides might be a natural alternative for the prevention/control of afb.

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Evaluation of some essential oils for the control and prevention of American Foulbrood disease in honey bees

Cited by 43 publications

References 15 publications

Screening of natural compounds for the control of nosema disease in honeybees (Apis mellifera)

Screening of natural compounds for the control of nosema disease in honeybees (Apis mellifera)

In vitro antibacterial effect of exotic plants essential oils on the honeybee pathogen Paenibacillus larvae, causal agent of American foulbrood

Potential Use of Buddleja Thyrsoides for the Control and Prevention of American Foulbrood Disease in Honey Bees

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