Evaluation of Specific Bacteriophage Against Salmonella infantis and Its Antibacterial Effects Compared to Ciprofloxacin in In Vitro Conditions

Abstract

10.30699/jambs.30.139.154Background & Objective: Phage therapy could be used as an alternative method to antibiotic treatments. The purpose of this study was to evaluate the antibacterial activities of isolated lytic bacteriophage against ciprofloxacin-resistant strain of Salmonella infanits in vitro conditions. Materials & Methods:The standard strain of Salmonella infantis and its specific bacteriophage was isolated by soft agar method. Phage susceptibility to heat and pH was evaluated by the Double-Layer Agar method. In vitro assay was carried out on human epithelial type 2 (HEp-2) cells to investigate the effect of bacteriophage on the cytotoxic and invasion of Salmonella infantis to human epithelial cells.Results: Head and tail morphology of bacteriophages against Salmonella infantis were identified by transmission electron microscopy and assigned to the Myoviridae family. The results of the double-layer agar assay showed that the titer of bacteriophages was 1.8×10 7 PFU/ml. bacteriophage was stable at 4 ֯ C and the best quantification of bacteriophage was determined at pH=8. The isolated bacteriophage was specific for Salmonella infantis and had no lytic activity against other pathogenic bacteria. In the evaluation of the binding and invasion of Salmonella infantis to the HEp-2 cell line, as expected, the lytic activity of specific bacteriophage was observed following inoculation. Conclusion:Additional studies are needed for better understanding of the interaction between phage, microorganisms and human host before applying phage therapy on a large scale.

In vitro bacteriolytic activity of Salmonella specific novel isolated bacteriophage

“…The continued development of antibiotic resistance is a global concern due to the unnecessary inappropriate use of antibiotics in human health and agricultural practices and their use as a feed additive to prevent bacterial diseases in the farm [7,9]. Therefore, due to the emergence of multidrug resistant Salmonella, there is a need for new alternative antibiotic administration strategies for effective control of Salmonella [10].…”