Evaluation of spray-dried lignin-based formulations and adjuvants as solar protectants for the granulovirus of the codling moth, Cydia pomonella (L)

Arthurs, Steven; Lacey, Lawrence A.; Behle, Robert W.

doi:10.1016/j.jip.2006.04.008

Cited by 52 publications

(32 citation statements)

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“…The authors Carneiro and Gomes (1997). Tamez-Guerra, Mdguire, Behle, Hamm, Sumner, and Shasha (2000) Horaczek and Viernstein (2004) Arthurs, Lacey, and Behle (2006) and Liu and Liu (2009a and b) made use of this technology to formulate biocontrol agents.…”

Section: Introductionmentioning

confidence: 99%

Compatibility of polymers to fungi Beauveria bassiana and Metarhizium anisopliae and their formulated products stability

et al. 2017

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ABSTRACT. Stability during storage is a limiting factor in the development and use of bioinsecticides. This study aims to evaluate the compatibility of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana with components used in encapsulated formulations and to evaluate shelf-life in formulations with B. bassiana. Sodium alginate and maltodextrin, hemicellulose, and dimethyl sulfoxide solvents were evaluated. Compatibility was evaluated by mixing the components with PDA, and the parameters evaluated were vegetative growth, sporulation and viability. The formulations of fungi were prepared in various concentrations of sodium alginate and then added drop-wise to calcium chloride. To evaluate the stability, formulations were stored at 26.5, 4.0, and -20.0°C. Monthly, samples were taken, and the capsules were placed on Petri dishes with PDA to verify the growth after seven days of incubation. These evaluations were performed by the 12 th month. Biopolymers evaluated were compatible with both fungi, exception maltodextrin at 1.5%, which was classified as moderately toxic to B. bassiana. Dimethyl sulfoxide was classified as moderately toxic to B. bassiana at 3.0% and to M. anisopliae at 2.0% and 3.0%. The formulations were stable throughout the 12 months in the conditions evaluated, while for the pure conidia of the fungus, 46% viability in the 6th month at 26.5°C was observed.Keywords: microbial control, formulations, entomopathogens, encapsulation, shelf-life, microbiology.Compatibilidade de polímeros aos fungos Beauveria bassiana e Metarhizium anisopliae e a estabilidade dos seus produtos formulados RESUMO. A estabilidade no armazenamento é um fator limitante ao desenvolvimento e uso de bioinseticidas. Este estudo objetivou avaliar a compatibilidade dos fungos entomopatogênicos M. anisopliae e B. bassiana com componentes utilizados em formulações encapsuladas e avaliar a estabilidade de formulações contendo B. bassiana bassiana. Alginato de sódio, maltodextrina, hemicelulose e o solvente dimetilsulfóxido foram avaliados. A compatibilidade foi avaliada misturando os componentes ao BDA e os parâmetros para avaliação foram crescimento vegetativo, esporulação e viabilidade. As formulações fúngicas foram preparadas em concentrações de alginato de sódio gotejadas em cloreto de cálcio. Para avaliar a estabilidade, as formulações foram armazenadas em 26,5; 4,0 e -20,0°C. Mensalmente, amostras foram sendo retiradas e as cápsulas colocadas em placas de Petri com BDA para verificar o crescimento após 7 dias de incubação. Essas avaliações ocorreram durante 12 meses. Os biopolímeros avaliados foram compatíveis a ambos os fungos, com exceção de maltodextrina a 1,5%, classificado como moderadamente tóxico para B. bassiana. O dimetilsulfóxido foi classificado como moderadamente tóxico para B. bassiana a 3,0%; e para M. anisopliae a 2,0 e 3,0%. As formulações permaneceram estáveis durante os 12 meses nas condições avaliadas, enquanto para o conídio puro, ao sexto mês já apresentava 46% de viabilidade a 26,5°C.Pa...

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Section: Introductionmentioning

confidence: 99%

Compatibility of polymers to fungi Beauveria bassiana and Metarhizium anisopliae and their formulated products stability

et al. 2017

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“…One property of lignin is the ability to absorb visible wavelengths of light, which has made it a prime candidate for a UV protectant. Arthurs et al (2006) reported spray-dried lignin-encapsulated CpGV formulations provided extended UV protection applied at a relatively high (3 x 1010 OB/L), but not low (3 x 108 OB/L) virus dosages. In a similar study, McGuire et al (2001) demonstrated formulations of Anagrapha falcifera Nuclear Polyhedrosis Virus (AfMNPV) containing lignin retained virus activity significantly longer than formulations without lignin.…”

Section: 4mentioning

confidence: 98%

“…Studies have demonstrated adjuvants providing UV protection have significantly improved the activity of baculoviruses and prolonged virus viability (Arthurs et al, 2006;Lacey and Shapiro-Ilan, 2003). Lignin is an abundant polymer derived from vascular plants and has been investigated as an adjuvant for microbial insecticides (Arthurs et al, 2006).…”

Section: 4mentioning

confidence: 99%

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Review of Vaccinia Virus and Baculovirus Viability Versus Virucides

Buckley¹,

O’Connell²,

Ouellette³

2008

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“…Inactivation of microencapsulated virus in the present study was 8.67% after 4 h of exposure to UVB light (302 nm); similar results were obtained by , who microencapsulated another isolation of this virus (NPV001) with Eudragit® S100 by solvent evaporation, which presented 13% inactivation following 4 h of irradiation with a ReptiGlo 8.0 lamp (33% UVA and 8% UVA), confirming the polymer protective effect against UV-light. Spray drying with other polymers as lignin has been used for baculovirus formulation improving OBs photostability as reported by Arthurs et al (2006) who encapsulated Cydia pomonella (CpGV) granulovirus. Behle and Popham (2012) also encapsulated two S. frugiperda NPVs by spray drying with lignin, obtaining 35% inactivation of microencapsulated virus irradiated with simulated solar light, while non-formulated virus reduced its insecticidal activity by more than 94%.…”

Section: Photostability Of Microencapsulated Virusmentioning

confidence: 99%

Microencapsulation of a Colombian Spodoptera frugiperdaNucleopolyhedrovirus with Eudragit® S100 by spray drying

Kurmen

Álvarez

Villamizar

2015

Braz. arch. biol. technol.

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Evaluation of spray-dried lignin-based formulations and adjuvants as solar protectants for the granulovirus of the codling moth, Cydia pomonella (L)

Cited by 52 publications

References 24 publications

<b>Compatibility of polymers to fungi <i>Beauveria bassiana</i> and </i>Metarhizium anisopliae</i> and their formulated products stability

<b>Compatibility of polymers to fungi <i>Beauveria bassiana</i> and </i>Metarhizium anisopliae</i> and their formulated products stability

Review of Vaccinia Virus and Baculovirus Viability Versus Virucides

Microencapsulation of a Colombian Spodoptera frugiperdaNucleopolyhedrovirus with Eudragit® S100 by spray drying

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