Evaluation of Stability of Surface-Treated Mini-Implants in Diabetic Rabbits

Abstract

Introduction. The purpose of this study was to investigate effects of surface treatment of mini-implants in diabetes-induced rabbits by comparing osseointegration around mini-implants. Methods. Twelve New Zealand white rabbits were divided into two groups (alloxan-induced diabetic group and control group). A total of 48 mini-implants were placed after four weeks of diabetic induction. 24 mini-implants were surface-treated with SLA (sandblasted with large grit, and acid etched) and the remaining 24 mini-implants had smooth surfaces. Four weeks after placement, 32 mini-implants were removed from 4 control and 4 diabetic rabbits. Insertion and removal torques were measured. The remaining 16 mini-implants from the two groups were histomorphometrically analyzed. Results. Maximum insertion torque showed no difference between diabetic and control groups, but total insertion energy was higher in control group. In surface-treated mini-implants, maximum removal torque was higher in both diabetic and control groups. Bone-implant contact (BIC) was increased in the control group when compared to the diabetic group. Surface-treated group had higher BIC than smooth surface group in both control and diabetic groups. However, there was no significantly statistical difference. Conclusions. Type 1 diabetes mellitus and surface treatment method of mini-implant affected primary stability of mini-implants. In addition, the use of orthodontic mini-implants in a diabetic patient is likely to show results similar to the healthy patient.

Efficacy of Bioactive Glass Nanofibers Tested for Oral Mucosal Regeneration in Rabbits with Induced Diabetes

“…The method that was used to induce diabetes mellitus in the rabbit animal model by alloxan monohydrate was accomplished after reviewing the literature and a preliminary study, as a minimal number of studies used rabbits as a model for diabetic research. In this study, we combined both techniques that were described by Wang et al [41] and Oh et al [42], in which we allowed animals to eat and drink before injection to avoid the postoperative hypoglycemic shock that proved to reduce the rate of animal death. Moreover, using a 10% solution of 150 mg/kg alloxan monohydrate enabled hyperglycemia after a single dose only, which was sustained until the end of the study.…”

“…A single dose of 10% (w/v) AMH solution was achieved by dissolving 150 mg/kg body weights in sterile normal saline. The solution was injected via the marginal ear vein using 26 gauge cannula (Lars medicare, Haryana, India) over a period of 30-60 s [42] (Figure 2a,b). After complete recovery, the animals were transferred back to their cages and food and water were offered immediately.…”

“…The blood glucose level was monitored every one hour for the first 7 h post-injection. Rabbits were subcutaneously injected with 20 mL of 5% glucose solution three to five times during the first two days to avoid post-operative hypoglycemic shock [42]. Contentious monitoring of blood glucose level was done every day for the first three days.…”

“…After that, blood glucose was measured once weekly until the end of the experiment. The rabbits were confirmed as diabetic if the blood glucose level remained >200 mg/dL for 15 days after induction [41,42]. Following animal euthanization, sections of pancreatic tissues were histologically examined [43].…”

Falhas biomecânicas com uso de mini-implante ortodôntico: análise por elementos finitos

“…Para um dos parâmetros frequentemente utilizado, o "bone implant contact" (BIC), cuja fórmula está apresentada abaixo, as maiores médias variaram entre 19,5% e 78,5% nos estudos de mini-implantes ortodônticos analisados (86,(89)(90)(91).…”