Application of irregular mass configuration in modern design era of buildings is unavoidable in order to serve various building functionality. The effect of mass irregularities was investigated on 5-storey portable moment resisting steel tower (PostFrame), against its dynamic behaviour. PostFrame was designed at five meter height and assembled on the strong floor in Jamilus Research Centre laboratory, UTHM. The predominant frequencies and mode shapes were determined on bare frame, uniform mass frame and mass irregularities’ frames. A total of four steel blocks at 800 kg were placed in uniform, ascending and alternating orientations on the first to fourth level of the PostFrame. Ambient vibration testing (AVT) was conducted using uni-axial accelerometer sensors. The sensors were aligned in bi-axial horizontal directions with respect to the North-South (NS) and East-West (EW) directions. ARTeMIS processing tool and Frequency Domain Decomposition (FDD) methods were used in the analysis of dynamic behaviour. Comparative findings were made between bare frame and mass action frame. Two translation mode shapes and torsional mode were illustrated by respective three predominant frequencies. Significant reduction of predominant frequency showed up to 37.79% between bare frame and frame with ascending mass orientation.Uniform mass orientation shows at the lowest percentage discrepancy percentage compared to others. Even though some changes have be found in predominant frequencies, but comparable mode shapes illustration were observed from all three predominant frequencies from all frame configurations.