Evaluation of Stem Cell Components in Retrocorneal Membranes

Lee, Seok Hyun; Kim, Kyoung Woo; Kim, Mi Kyung; Chun, Yeoun Sook; Kim, Jae Chan

doi:10.3346/jkms.2014.29.6.846

Cited by 6 publications

(1 citation statement)

References 20 publications

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“…Retrocorneal membranes (RCMs) can develop after a variety of corneal affections, like penetrating or chemical trauma, corneal surgery, and inflammatory diseases 1 , 2 , 3 , 4 . They can be observed in up to 7% of eyes after penetrating corneal injuries and, furthermore, are related to nearly half of all cases with failed penetrating keratoplasty 5 , 6 , 7 . In affected eyes, they represent a severe threat to eyesight and may cause blindness.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Findings in Retrocorneal Membranes of Eyes with Corneal Decompensation after Complicated Intraocular Surgery

Gerding

Schlageter²,

Zettl³

et al. 2023

Klin Monbl Augenheilkd

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Background Retrocorneal membranes (RCMs) may result from epithelial ingrowth, stromal keratocytic downgrowth, fibrous metaplasia of the corneal endothelium, or a combination of these processes. In an institutional case series, the clinical history, ocular findings, and immunohistochemical staining results of RCMs were analysed in patients with unilateral corneal decompensation after complicated intraocular surgery. Methods and Patients Between January 2021 and September 2022, six retrocorneal membranes were excised during Descemetʼs stripping automated endothelial keratoplasty (DSAEK) and Descemet membrane endothelial keratoplasty (DMEK) procedures and classified after screening with haematoxylin and eosin, periodic acid-Schiff, elastic van Gieson staining, and immunohistochemical screening with cytokeratin 7 (CK7), anti-cytokeratin (CAM5.2 and AE1/3), cell surface glycoprotein CD34, smooth muscle actin (α-SMA), and vimentin. Results On the basis of the immunohistochemical screening, the majority of excised RCMs (5 of 6) could histopathologically be classified as membranes originating from fibrous metaplasia of the corneal endothelium. All these RCMs were positive for CK7, α-SMA, and vimentin and negative for CAM5.2 and CD34. In one patient, an RCM had developed after 18 days of corneal contact to a free-floating dexamethasone implant in the anterior chamber and was classified as originating from stromal keratocyte downgrowth (α-SMA- and vimentin-positive, all others negative). All eyes in this series had a previous history of complicated cataract surgery, partially with subsequent intraocular lens exchange. No eyes after previous penetrating keratoplasty were in this series. Conclusions In this series of eyes with previous complicated intraocular interventions (in most cases cataract surgery and revisions), the dominating RCM belonged to the type originating from fibrous metaplasia of the corneal endothelium.

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