Evaluation of stool microbiota signatures in two cohorts of Asian (Singapore and Indonesia) newborns at risk of atopy

Yap, Gaik Chin; Chee, K.; Hong, Pei‐Ying; Lay, Christophe; Satria, Cahya Dewi; Sumadiono,; Soenarto, Yati; Haksari, Ekawaty Lutfia; Aw, Marion; Shek, Lynette Pei‐Chi; Chua, Kaw Yan; Zhao, Yingxin; Leow, Doreen; Lee, Bee Wah

doi:10.1186/1471-2180-11-193

Cited by 40 publications

(44 citation statements)

References 51 publications

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“…antibiotic use in infancy) while being mindful of the relatively small number of subjects within microbiota profiles. These variables were chosen based on clinical plausibility and a priori knowledge . In the sensitivity analysis, we repeated the multivariable model with the number of siblings as the ordinal variable.…”

Section: Methodsmentioning

confidence: 99%

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Household siblings and nasal and fecal microbiota in infants

Hasegawa

Linnemann

Mansbach

et al. 2016

Pediatrics International

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Background Early-life exposure to older siblings is associated with a lower risk of asthma. To date, no study has addressed the impact of having siblings on both the airway and fecal microbiota during infancy. We aimed to profile the nasal airway and fecal microbiota in infants, and to examine the association between having siblings and microbiota profiles. Methods We conducted a cross-sectional study of 105 healthy infants (aged <1 year). By applying 16S rRNA gene sequencing and an unbiased clustering approach to the nasal airway and fecal samples, we identified microbiota profiles and then determined the association between having siblings and microbiome profiles. Results Overall, the median age was 3.4 months (IQR, 2.0–4.7 months); 43% had siblings in the household. Unbiased clustering of nasal airway microbiota identified three profiles: Moraxella-dominant (43%), Corynebacterium/Dolosigranulum-dominant (36%), and mixed (21%). Infants with siblings were more likely to have Moraxella-dominant profile than Corynebacterium/Dolosigranulum-dominant profile (76% vs. 18%) while those without siblings had the opposite pattern (18% vs. 50%) (multivariable-adjusted P<0.001). Fecal microbiota consisted of three profiles: Bifidobacterium-dominant (39%), Escherichia-dominant (31%), and Enterobacter-dominant (30%). Infants with siblings were more likely to have Bifidobacterium-dominant profile than Escherichia-dominant profile (49% vs. 24%) while those without siblings had the opposite pattern (32% vs. 37%) (multivariable-adjusted P=0.04). Conclusions In this cross-sectional study, we found that infants with siblings were more likely to have Moraxella-dominant nasal microbiota profile and Bifidobacterium-dominant fecal microbiota profile. Our findings should facilitate further investigation of the interplay between early-life environmental exposures, the microbiome, and childhood asthma.

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Section: Methodsmentioning

confidence: 99%

“…To date, most microbiome research has focused on the gut, and, with regard to the previous comments, the possible link between the presence of older siblings and specific fecal microbiota . The airway, however, is a major portal for microbial exposure, and the airway microbiota has been found to influence immune response in patients with asthma .…”

mentioning

confidence: 99%

Household siblings and nasal and fecal microbiota in infants

Hasegawa

Linnemann

Mansbach

et al. 2016

Pediatrics International

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“…Indeed, several lines of evidence have shown that intestinal dysbiosis (imbalance of microbiota) is a feature of chronic disease as well as diet. Population differences in microbiota composition are well known; the profiles of gut bacteria in American, European, and Asian cohorts are quantifiably different [52–54]. Furthermore, gut microbiota is altered in allergic diseases and other chronic inflammatory disorders such as Crohn's disease [55].…”

Section: Gut Microbiota Short-chain Fatty Acids and Healthmentioning

confidence: 99%

Histone Deacetylase Inhibition and Dietary Short-Chain Fatty Acids

2011

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Changes in diet can also have dramatic effects on the composition of gut microbiota. Commensal bacteria of the gastrointestinal tract are critical regulators of health and disease by protecting against pathogen encounter whilst also maintaining immune tolerance to certain allergens. Moreover, consumption of fibre and vegetables typical of a non-Western diet generates substantial quantities of short-chain fatty acids (SCFAs) which have potent anti-inflammatory properties. Dietary interventions such as probiotic supplementation have been investigated for their pleiotropic effects on microbiota composition and immune function. Probiotics may restore intestinal dysbiosis and improve clinical disease through elevated SCFA levels in the intestine. Although the precise mechanisms by which such dietary factors mediate these effects, SCFA metabolites such as butyrate also function as histone deacetylase inhibitors (HDACi), that can act on the epigenome through chromatin remodeling changes. The aim of this review is to provide an overview of HDAC enzymes and to discuss the biological effects of HDACi. Further, we discuss the important relationship between diet and the balance between health and disease and how novel dietary interventions such as probiotics could be alternative approach for the prevention and/or treatment of chronic inflammatory disease through modulation of the intestinal microbiome.

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“…Moreover, the presence of pathogenic species, or absence of beneficial species, in early childhood has been suggested to play a key role in the initiation of preterm birth [12], development of asthma or eczema [13], [14], allergy [15], autism [16] or other immunological deficiency [17], [18].…”

Section: Introductionmentioning

confidence: 99%

“…However, despite the growing recognition that commensal microbes may contribute fundamentally to infant and childhood development and immunity [14], [15], [17], [18], [28], [29], only a few studies have determined the microbial composition of the first intestinal discharge, or meconium, in premature [30]–[32] and in term neonates [24], [25] and linked its bacterial content to maternal eczema and infant mucus congestion during the first year of life [24]. Therefore, the main objectives of this study were to further characterize the composition of the meconium and assess whether maternal diabetes status, prior to or during pregnancy, affects bacterial composition of the newborn's first stool.…”

Section: Introductionmentioning

confidence: 99%

Diversified Microbiota of Meconium Is Affected by Maternal Diabetes Status

et al. 2013

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ObjectivesThis study was aimed to assess the diversity of the meconium microbiome and determine if the bacterial community is affected by maternal diabetes status.MethodsThe first intestinal discharge (meconium) was collected from 23 newborns stratified by maternal diabetes status: 4 mothers had pre-gestational type 2 diabetes mellitus (DM) including one mother with dizygotic twins, 5 developed gestational diabetes mellitus (GDM) and 13 had no diabetes. The meconium microbiome was profiled using multi-barcode 16S rRNA sequencing followed by taxonomic assignment and diversity analysis.ResultsAll meconium samples were not sterile and contained diversified microbiota. Compared with adult feces, the meconium showed a lower species diversity, higher sample-to-sample variation, and enrichment of Proteobacteria and reduction of Bacteroidetes. Among the meconium samples, the taxonomy analyses suggested that the overall bacterial content significantly differed by maternal diabetes status, with the microbiome of the DM group showing higher alpha-diversity than that of no-diabetes or GDM groups. No global difference was found between babies delivered vaginally versus via Cesarean-section. Regression analysis showed that the most robust predictor for the meconium microbiota composition was the maternal diabetes status that preceded pregnancy. Specifically, Bacteroidetes (phyla) and Parabacteriodes (genus) were enriched in the meconium in the DM group compared to the no-diabetes group.ConclusionsOur study provides evidence that meconium contains diversified microbiota and is not affected by the mode of delivery. It also suggests that the meconium microbiome of infants born to mothers with DM is enriched for the same bacterial taxa as those reported in the fecal microbiome of adult DM patients.

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Evaluation of stool microbiota signatures in two cohorts of Asian (Singapore and Indonesia) newborns at risk of atopy

Cited by 40 publications

References 51 publications

Household siblings and nasal and fecal microbiota in infants

Household siblings and nasal and fecal microbiota in infants

Histone Deacetylase Inhibition and Dietary Short-Chain Fatty Acids

Diversified Microbiota of Meconium Is Affected by Maternal Diabetes Status

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