Evaluation of Storage Conditions and Bacterial Proliferation in Blood Components

Sharma, Ratti Ram; Subramanian, Papagudi Ganesan; Shubham, Kumar; Singh, Mitasha; Sharma, Mukut; Agnihotri, S. K.; Marwaha, Neelam

doi:10.1309/auhe16wn6mjc4v67

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…In the present study it was seen that contamination of blood by gram positive bacteria was within few days of storage while contamination by gram negative bacteria was delayed. Our findings are in agreement with (Bolarinwa et al, 2011) and (Sharma et al, 2004) Gram-positive isolates being commensal or transient skin flora, contamination is thought to occur primarily during phlebotomy, as a result of incomplete disinfection and/or skin core removal by the collection needle. Therefore they are isolated soon after donation, whereas Gram negative organisms not usually detectable until after a period of proliferation during storage.…”

Section: Resultssupporting

confidence: 93%

Bacterial Contamination of Donor Blood and Blood Components from a Tertiary Care Hospital in North India

Sharma¹,

Mahajan²

2018

Int.J.Curr.Microbiol.App.Sci

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Screening of blood donors for viral pathogens has greatly improved the safety of donated blood. However, transfusion associated bacterial sepsis, remains an important public health concern, which has received very little attention. Therefore this study was carried out to determine the prevalence of bacterial contamination in donor blood and blood products, to find the commonly contaminated blood product and to identify the microorganisms involved. The present study was conducted on 136 random blood samples received in the Department of Microbiology, GMC, Jammu for a period of 1 year i.e. April 2017-2018. Bacteria were identified using standard bacteriological and biochemical methods. The overall prevalence rate was 12.50% (Packed cells, 21.21%; Platelets, 10.41%; Whole blood 9.09%). The most commonly isolated bacteria were Klebsiella sp, Staph. aureus and CONS. Most of the contaminated samples had 3-7 days of storage time. Maximum number of contaminated samples was from Blood Bank, GMC, Jammu. This concludes that bacterial contamination of donor blood and blood components is common in our hospital setting. Active surveillance methods to improve the safety of transfusion, regular monitoring and educating the clinical staff can help in reducing the contamination of transfusion blood.

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Section: Resultssupporting

confidence: 93%

Bacterial Contamination of Donor Blood and Blood Components from a Tertiary Care Hospital in North India

Sharma¹,

Mahajan²

2018

Int.J.Curr.Microbiol.App.Sci

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“…However, incidence of bacterial contamination has not changed significantly. 3 Transfusion of bacterially contaminated blood and blood products may have severe and even fatal consequences. [4][5][6] The index of bacterial contamination is a measure of transfusion safety in blood banks.…”

Section: Sirmentioning

confidence: 99%

Evaluation of bacterial contamination of blood components in a tertiary care centre

Sehgal¹,

Prakhya

2022

Bangladesh J Med Sci

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“…Controlling temperature by temperature indicators or time–temperature integrator units attached to the surface of the RBC pouch implies that surface temperature well represents sample’s core temperature, which can but doesn’t have to be true [10], [11], [12], [13], [14], [15]. Facing worldwide decreased availability of lifesaving RBC challenges a more precise evaluation of correlations between maximum (surface), mean and minimum (core) temperature in RBC units removed from stock to prevent unnecessary wastage of blood components and, if transfused, ensure minimal risk to patients [1], [2], [3], [16], [17].…”

Section: Introductionmentioning

confidence: 99%

Thermometry of Red Blood Cell Concentrate: Magnetic Resonance Decoding Warm Up Process

Reiter

Kozma

et al. 2013

PLoS ONE

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PurposeTemperature is a key measure in human red blood cell concentrate (RBC) quality control. A precise description of transient temperature distributions in RBC units removed from steady storage exposed to ambient temperature is at present unknown. Magnetic resonance thermometry was employed to visualize and analyse RBC warm up processes, to describe time courses of RBC mean, surface and core temperatures by an analytical model, and to determine and investigate corresponding model parameters.MethodsWarm-up processes of 47 RBC units stored at 1–6°C and exposed to 21.25°C ambient temperature were investigated by proton resonance frequency thermometry. Temperature distributions were visualized and analysed with dedicated software allowing derivation of RBC mean, surface and core temperature-time courses during warm up. Time-dependence of mean temperature was assumed to fulfil a lumped capacitive model of heat transfer. Time courses of relative surface and core temperature changes to ambient temperature were similarly assumed to follow shifted exponential decays characterized by a time constant and a relative time shift, respectively.ResultsThe lumped capacitive model of heat transfer and shifted exponential decays described time-dependence of mean, surface and core temperatures close to perfect (mean R2 were 0.999±0.001, 0.996±0.004 and 0.998±0.002, respectively). Mean time constants were τ mean = 55.3±3.7 min, τ surface = 41.4±2.9 min and τ core = 76.8±7.1 min, mean relative time shifts were Δsurface = 0.07±0.02 and Δcore = 0.04±0.01. None of the constants correlated significantly with temperature differences between ambient and storage temperature.ConclusionLumped capacitive model of heat transfer and shifted exponential decays represent simple analytical formulas to describe transient mean, surface and core temperatures of RBC during warm up, which might be a helpful tool in RBC temperature monitoring and quality control. Independence of constants on differences between ambient and storage temperature suggests validity of models for arbitrary storage and ambient temperatures.

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Evaluation of Storage Conditions and Bacterial Proliferation in Blood Components

Cited by 5 publications

References 24 publications

Bacterial Contamination of Donor Blood and Blood Components from a Tertiary Care Hospital in North India

Bacterial Contamination of Donor Blood and Blood Components from a Tertiary Care Hospital in North India

Evaluation of bacterial contamination of blood components in a tertiary care centre

Thermometry of Red Blood Cell Concentrate: Magnetic Resonance Decoding Warm Up Process

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