Evaluation of stored potato (Solanum tuberosum L.) for soft rot bacteria in Ibadan, Nigeria

Abiodun, Durojaye Hammed; Abisola, Owoeye Tolulope

doi:10.11594/jaab.02.01.07

Cited by 2 publications

(5 citation statements)

References 13 publications

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“…2 Pectobacterium actinidiae strain KKH3, 3 Pectobacterium carotovorum strain DSM 30168, 4 Pectobacterium carotovorum strain ATCC 15713, 5 Bacillus amyloliquefaciens strain BCRC, 6 Bacillus amyloliquefaciens DSM 7 = ATCC 23350, 7 Bacillus subtilis subsp. subtilis strain 168, 8 Bacillus subtilis strain BCRC 10255, 9 10 Bacillus subtilis strain JCM 1465, 11 Bacillus subtilis strain IAM 12118, 12 Bacillus subtilis strain NCDO 1769, 12 Bacillus tropicus strain MCCC 1A01406, 13 Verticiella sediminum strain XH089, 14 Achromobacter marplatensis strain R-46660, 15 Achromobacter xylosoxidans strain Hugh 2838, 17 Achromobacter insuavis strain LMG 26845, 19 Achromobacter xylosoxidans strain NBRC 15126, 20 Achromobacter denitrificans strain NBRC 15125…”

Section: Discussionmentioning

confidence: 99%

“…Nutrient agar was used for the isolation of the soft rot pathogen as used earlier by Abiodun & Abisola, [14].…”

Section: Media Preparationmentioning

confidence: 99%

“…Colony features such as growth characteristics, colour, margin shape and appearance were evaluated. Each colony was compared to the structure for the bacterial strains about the existing taxa in a standard manual of determinative bacteriology as described by Abiodun & Abisola [14]. Microscopy was done using computer software (ImageFocus Plus V2, YM.2013) on laboratory microscope, (BlueBio: German Manufacturers).…”

Section: Morphological Characterization Of Isolated Soft Rot Strainsmentioning

confidence: 99%

“…However, the method is biased on the ability of bacteria to change their physiology based on changing environmental conditions. Morphological characterization may also underestimate viable organisms which are difficult to grow in in vitro cultures [14,15]. Biochemical characterization distinguishes pathogen strains on their nutritional and metabolic capabilities thus, provides an insight into the binding and action mechanism.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Identification of Bacterial Soft-Rot-Causing Isolates from Cassava, Homa-Bay County, Kenya

Otieno D.,

Muraya M. M.,

Mungiria J. N.

2023

IJPR

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Cassava bacterial soft rot is of great importance to economically valuable crop varieties. Commonly, bacterial soft rot strains cause destructive vegetable and potato diseases worldwide. The strain act similar to other species of the family Enterobacteriaceae that cause soft rot in plants. However, the disease has spread in a wide range of environment with its prevalence and causative strains less known to most agroecological zones of Kenya. Generally, aim of this study was to isolate, characterize and identify bacterial soft rot causative strains in cassava, Homa-Bay County. At the Sub County level, 104 farms were identified through a simple random method and symptomatic cassava plants sampled along laid transect in each farm. Rotten cassava tubers were then randomly sampled, packed and taken to the laboratory for pathogen identification. The bacterial strains were isolated in Nutrient Agar at 37˚c for 24 hours to obtain pure cultures. Identification was done using morphological, biochemical and 16S rRNA analysis method. The study identified: two Pectobacterium carotovorum, three Bacillus species: Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus tropicus, and three Achromobacter species: Achromobacter marplatensis, Verticiella sediminum, and Achromobacter xylosoxidans, pathogenic strains causing cassava bacterial soft rot. In this study, characterization and identification of cassava bacterial soft rot in Homa Bay County was done for the first time. The results can not only be used in the characterization enzymes produced by cassava bacterial soft rot strains but also in the development of effective control strategy.

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Section: Discussionmentioning

confidence: 99%

“…Nutrient agar was used for the isolation of the soft rot pathogen as used earlier by Abiodun & Abisola, [14].…”

Section: Media Preparationmentioning

confidence: 99%

Section: Morphological Characterization Of Isolated Soft Rot Strainsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and Identification of Bacterial Soft-Rot-Causing Isolates from Cassava, Homa-Bay County, Kenya

Otieno D.,

Muraya M. M.,

Mungiria J. N.

2023

IJPR

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“…Subculture were subsequently carried out to obtained pure culture of each isolate for further studies. Pure culture of individual isolates was kept at 40c for biochemical test (Muhammad et al, 2018;Abiodun and Abisola, 2021).…”

Section: Sample Preparation/isolation Of Bacterialmentioning

confidence: 99%

Preliminary Identification of Bacterial Species Contaminating Okra Fruits in Some Selected Markets in Owo, Nigeria

O. A,

2024

JNFP

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In spite of Okra (Abelmoschus esculentus L.) cultivation relevance in the society, management of its post-harvest disease has stand out as major issue to plant pathologists. This study therefore reports findings on the spoilage bacteria associated with Okra fruit during post-harvest storage. Okra sample was purchased from three (3) local markets (Ogwagbe market, Areghona market and Oba market) in Owo local government and taken to the Plant Pathology Laboratory of the Department of Crops Soil and Pest Management, Federal University of Technology, Akure. About 2g of deteriorated Okra fruit sample was sterilized and aseptically transfer into the sterile 20cm2 beaker. The bacteria isolated were incubated at 37+ 20c for 24hours in a Nutrient Agar (NA) plate. At the end of incubation period the Colonies forming units from violet bacteria cells were counted and recorded appropriately per gram of each of the deteriorating Okra sample. Pure culture of individual isolates was kept at 40c for biochemical test and isolates were identified. A total of 26 bacteria were isolated and identified from the Okra sample from the 3 local markets on the basis of colony morphology and the staining characteristics. Location A has the highest population of bacteria isolate while location B and C had lowest bacterial population. The highest occurring genera can be found in Micrococcus, Bacillus and Staphylococcus followed by Alcaligenes, Pediococcus, and Erwinia. Alcaligenes odorans and Bacillus pumilus are the most occurring species of bacteria isolated from the market. Total viable counts ranges from 1.20 X 10-8 (cfu/g) to 1.72 X 10-8 (cfu/g). The highest average coliform forming unit occurs in Location A (1.52 X 10-8 (cfu/g)) while the least average coliform forming unit occurred in Location B (1.23 X 10-8 (cfu/g)). Alcaligenes odorans Bacillus pumilus and Micrococcus spp. had the highest frequency of occurrence (67 %). The presence of these bacteria on Okra fruit are potential threat to its production most importantly coliforms, pose a serious threat to consumers' health as the bacteria could produce toxins, which are poisonous when consumed. Proper handling methods should be adopted by both farmer and consumers to minimize the bacterial deterioration of okra fruit in the study area.

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Evaluation of stored potato (Solanum tuberosum L.) for soft rot bacteria in Ibadan, Nigeria

Cited by 2 publications

References 13 publications

Isolation and Identification of Bacterial Soft-Rot-Causing Isolates from Cassava, Homa-Bay County, Kenya

Isolation and Identification of Bacterial Soft-Rot-Causing Isolates from Cassava, Homa-Bay County, Kenya

Preliminary Identification of Bacterial Species Contaminating Okra Fruits in Some Selected Markets in Owo, Nigeria

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