Evaluation of streptomycete actinobacterial isolates as biocontrol agents against royal poinciana stem canker disease caused by the fungal pathogen Neoscytalidium dimidiatum

Raish, Seham M. Al; Saeed, Esam Eldin; Alyafei, Dana M.; El‐Tarabily, Khaled A.; AbuQamar, Synan F.

doi:10.1016/j.biocontrol.2021.104783

Cited by 23 publications

(9 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As suggested by [71], actinobacterial isolates were first identified according to their in vitro abilities to produce either CWDEs or diffusible antifungal metabolites against F. solani. Our data confirmed previous reports about the importance of actinobacteria as effective BCAs against phytopathogens [5,6,16,72]. To eliminate weak BCA isolates obtained from the primary in vitro screening assays, potential actinobacterial antagonists to F. solani were tested for a period of 12 weeks for their endophytic abilities to grow and increase in numbers in root tissues of date palm under greenhouse conditions.…”

Section: Discussionsupporting

confidence: 87%

Biocontrol Potential of Endophytic Actinobacteria against Fusarium solani, the Causal Agent of Sudden Decline Syndrome on Date Palm in the UAE

Alblooshi

Purayil

Saeed

et al. 2021

JoF

Self Cite

View full text Add to dashboard Cite

Thirty-one endophytic streptomycete and non-streptomycete actinobacteria were isolated from healthy date palm root tissues. In vitro screening revealed that the antifungal action of isolate #16 was associated with the production of cell-wall degrading enzymes, whereas with diffusible antifungal metabolites in isolate #28, albeit their production of volatile antifungal compounds. According to the 16S rRNA gene sequencing, isolates #16 and #28 were identified as Streptomyces polychromogenes UAE2 (Sp; GenBank Accession #: OK560620) and Streptomyces coeruleoprunus UAE1 (Sc; OK560621), respectively. The two antagonists were recovered from root tissues until 12 weeks after inoculation, efficiently colonized root cortex and xylem vessels, indicating that the date palm roots are a suitable habitat for these endophytic isolates. At the end of the greenhouse experiments, the development of sudden decline syndrome (SDS) was markedly suppressed by 53% with the application of Sp and 86% with Sc, confirming their potential in disease management. Results showed that the estimated disease severity indices in diseased seedlings were significantly (p < 0.05) reduced from 4.75 (scale of 5) to 2.25 or 0.67 by either Sp or Sc, respectively. In addition, conidial numbers of the pathogen significantly (p < 0.05) dropped by 38% and 76% with Sp and Sc, respectively, compared to infected seedlings with F. solani (control). Thus, the suppression of disease symptoms was superior in seedlings pre-inoculated with S. coeruleoprunus, indicating that the diffusible antifungal metabolites were responsible for F. solani retardation in these plants. This is the first report of actinobacteria naturally existing in date palm tissues acting as microbial antagonists against SDS on date palm.

show abstract

Section: Discussionsupporting

confidence: 87%

Biocontrol Potential of Endophytic Actinobacteria against Fusarium solani, the Causal Agent of Sudden Decline Syndrome on Date Palm in the UAE

Alblooshi

Purayil

Saeed

et al. 2021

JoF

Self Cite

View full text Add to dashboard Cite

show abstract

“…being the most studied particularly as biocontrol agents to contrast various bacteria and fungi causing plant diseases [ 48 , 49 , 50 , 51 , 52 , 53 ], has been described. These studies highlighted the production of an arsenal of different bacterial volatile and non-volatile bioactive molecules that can promote plant growth by increasing tolerance against abiotic and biotic stresses and by stimulating plant development as inferred from the scientific literature describing their action mechanisms [ 51 , 54 , 55 ]. For example, the fermentation broth of Streptomyces sp.…”

Section: Discussionmentioning

confidence: 99%

Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers

et al. 2023

View full text Add to dashboard Cite

The use of chemical fertilizers and pesticides has caused harmful impacts on the environment with the increase in economic burden. Biofertilizers are biological products containing living microorganisms capable of improving plant growth through eco-friendly mechanisms. In this work, three actinobacterial strains Streptomyces violaceoruber, Streptomyces coelicolor, and Kocuria rhizophila were characterized for multiple plant growth promoting (PGP) traits such as indole acetic acid production, phosphate solubilization, N2-fixation, and drought and salt tolerance. Then, these strains were investigated for their secreted and cellular metabolome, revealing a rich arsenal of bioactive molecules, including antibiotics and siderophores, with S. violaceoruber being the most prolific strain. Furthermore, the in vivo assays, performed on tomato (Solanum lycopersicum L.), resulted in an improved germination index and the growth of seedlings from seeds treated with PGP actinobacteria, with a particular focus on S. violaceoruber cultures. In particular, this last strain, producing volatile organic compounds having antimicrobial activity, was able to modulate volatilome and exert control on the global DNA methylation of tomato seedlings. Thus, these results, confirming the efficacy of the selected actinobacteria strains in promoting plant growth and development by producing volatile and non-volatile bioactive molecules, can promote eco-friendly alternatives in sustainable agriculture.

show abstract

“…Understanding the appropriate conditions for implementing proper programs against plant pathogens requires collaborations between different research groups focusing on the mechanisms associated with MBCAs to manage diseases on plants. In the last two decades, extensive research has focused on the antifungal effect, rhizosphere colonization, and crop benefits linked to MBCA ( Compant et al, 2010 ; Al Hamad et al, 2021 ; Al Raish et al, 2021 ; Alwahshi et al, 2022 ). Thus, the products of MBCA on plant fungal pathogens and their impacts on plants are illustrated in Figure 2 .…”

Section: Mechanisms Used By Mbcasmentioning

confidence: 99%

Plant growth-promoting microorganisms as biocontrol agents of plant diseases: Mechanisms, challenges and future perspectives

et al. 2022

Self Cite

View full text Add to dashboard Cite

Plant diseases and pests are risk factors that threaten global food security. Excessive chemical pesticide applications are commonly used to reduce the effects of plant diseases caused by bacterial and fungal pathogens. A major concern, as we strive toward more sustainable agriculture, is to increase crop yields for the increasing population. Microbial biological control agents (MBCAs) have proved their efficacy to be a green strategy to manage plant diseases, stimulate plant growth and performance, and increase yield. Besides their role in growth enhancement, plant growth-promoting rhizobacteria/fungi (PGPR/PGPF) could suppress plant diseases by producing inhibitory chemicals and inducing immune responses in plants against phytopathogens. As biofertilizers and biopesticides, PGPR and PGPF are considered as feasible, attractive economic approach for sustainable agriculture; thus, resulting in a “win-win” situation. Several PGPR and PGPF strains have been identified as effective BCAs under environmentally controlled conditions. In general, any MBCA must overcome certain challenges before it can be registered or widely utilized to control diseases/pests. Successful MBCAs offer a practical solution to improve greenhouse crop performance with reduced fertilizer inputs and chemical pesticide applications. This current review aims to fill the gap in the current knowledge of plant growth-promoting microorganisms (PGPM), provide attention about the scientific basis for policy development, and recommend further research related to the applications of PGPM used for commercial purposes.

show abstract

Evaluation of streptomycete actinobacterial isolates as biocontrol agents against royal poinciana stem canker disease caused by the fungal pathogen Neoscytalidium dimidiatum

Cited by 23 publications

References 60 publications

Biocontrol Potential of Endophytic Actinobacteria against Fusarium solani, the Causal Agent of Sudden Decline Syndrome on Date Palm in the UAE

Biocontrol Potential of Endophytic Actinobacteria against Fusarium solani, the Causal Agent of Sudden Decline Syndrome on Date Palm in the UAE

Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers

Plant growth-promoting microorganisms as biocontrol agents of plant diseases: Mechanisms, challenges and future perspectives

Contact Info

Product

Resources

About