Evaluation of surgical margins required for complete excision of cutaneous mast cell tumors in dogs

Simpson, Amelia M.; Ludwig, Lori; Newman, Shelley J.; Bergman, Philip J.; Hottinger, Heidi; Patnaik, A. K.

doi:10.2460/javma.2004.224.236

Cited by 132 publications

(184 citation statements)

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“…Este é um aspecto bastante importante no delineamento das margens cirúrgicas, toda vez que o procedimento cirúrgico for considerado como tratamento. A margem cirúrgica tem sido considerada um dos critérios para estabelecer o prognós-tico para os mastocitomas (Seguin et al 2001, Thamm & Vail 2007, Dobson et al 2002, Michels et al 2002, Simpson et al 2004. A obliteração da gordura observado em alguns casos deve ser considerada como suspeita, podendo representar inflamação, neovascularização ou mesmo infiltrado neoplásico para o panículo adiposo (Webb 2000).…”

Section: Discussionunclassified

“…Muitos fatores estão envolvidos no seu prognós-tico, como a localização (Sfiligoi et al 2005, Welle et al 2008, margem cirúrgica (Michels et al 2002, Simpson et al 2004, atividade mitótica (Elston et al 2009), grau histológico (Macy, 1986), estadiamento (Thamm & Vail 2007), alguns marcadores moleculares, como Ki-67, PCNA, AgNOR (Scase et al 2006, Maglennon et al 2008), a expressão KIT pelos mastócitos (Thompson et al 2011) e a densidade vascular da lesão (Preziosi et al 2004).…”

Section: Tomografia Computadorizada De Mastocitomas Em Cãesunclassified

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Tomografia computadorizada de mastocitomas em cães: avaliação pré e pós-tratamento quimioterápico

et al. 2013

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Foram avaliados, por meio da tomografia computadorizada, 19 cães com mastocitomas tratados com quimioterapia. Aspectos como contorno, atenuação, realce pós-contraste e presença de clivagem com estruturas adjacentes foram avaliados. Aplicaram-se os critérios RECIST e a mensuração volumétrica das lesões para se avaliar a resposta ao tratamento. Quanto à atenuação, os mastocitomas se mostraram como lesões homogêneas ou heterogêneas, com tendência a limites definidos e contornos regulares e apresentaram realce moderado após administração do contraste iodado intravenoso. Os métodos RECIST e a mensuração volumétrica apresentaram uma excelente concordância na classificação da resposta terapêutica, fornecendo um bom parâmetro da resposta ao tratamento instituído. O exame de tomografia computadorizada se mostrou útil na delimitação do tumor e importante ferramenta no planejamento das margens cirúrgicas.

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Section: Discussionunclassified

Section: Tomografia Computadorizada De Mastocitomas Em Cãesunclassified

Tomografia computadorizada de mastocitomas em cães: avaliação pré e pós-tratamento quimioterápico

et al. 2013

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“…24 In general, it is believed that tumor excision using a 2-cm lateral and a deep margin of one fascial plane are adequate to completely excise G1 and G2 MCTs in dogs. 3,33 However, many veterinary oncologists ask for more accurate prognostic factors in cases of incomplete surgical excision. In the present study, the classical histological evaluation of MCT SM and c-KIT gene expression profile were measured concurrently and might be of prognostic usefulness.…”

Section: Discussionmentioning

confidence: 99%

c-KIT messenger RNA and protein expression and mutations in canine cutaneous mast cell tumors

et al. 2011

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Abstract. Cutaneous mast cell tumors (MCTs) are among the most common neoplasms in dogs and show a highly variable biologic behavior. Histological grading, cell proliferation markers, and KIT immunohistochemistry are typically used to predict post-surgical prognosis. In the present study, c-KIT messenger RNA (mRNA) expression was measured in canine MCTs and its relationship with tumor grade, immunohistochemical staining pattern, post-surgical prognosis, and mutations was investigated. A significant increase of c-KIT mRNA was observed in MCTs versus healthy skin and surgical margins. Mutations were observed in 8.3% of cases. The KIT staining pattern was investigated for both grading systems. In particular, staining pattern III was associated with grade II (G2) and G3 MCTs, while staining patterns I and II were associated with G1 and G2 MCTs. Considering the 2-tier histological grading, the high grade was mainly associated with pattern III (71%) while the low grade was associated with patterns II (70%) and I (28%). A weak association between the KIT staining pattern and outcome was also observed. The results obtained suggest that c-KIT mRNA is overexpressed in canine MCT, although the fold variations were not associated with the protein localization or complementary DNA mutations. These observations suggested that the 3 events were independent. The histological grading and the KIT staining pattern have prognostic value as previously published. Staining pattern I could be especially helpful in predicting a good prognosis of G2 MCTs. Sequence mutations were not necessarily suggestive of a worse prognosis, but might be useful in choosing a chemotherapy protocol.

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“…A variety of recommendations as to sufficient margins for surgical cure have been issued for different neoplasms. For example, 2 cm surgical margins are recommended for the surgical cure of grade I and II mast cell tumors (Fulcher et al, 2006; Simpson et al, 2004). Histologic evaluation of surgical margins has been used to determine the likelihood of recurrence for soft tissue sarcomas and carcinomas (Scarpa et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the effect of formalin fixation on skin specimens in dogs and cats

Miller

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2014

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Skin and subcutaneous tissues are the origin of most common neoplasms affecting dogs, accounting for approximately one third of all tumors encountered in the species. Surgical excision is frequently the best chance for a cure; determining factors influencing the success of excision are vital for surgical management of cases. This work examined the shrinkage of skin of various lengths from three sites in formalin for both dogs and cats. Tissues were measured on the animal (initial measurement), at the time of excision (post-removal), and after formalin fixation (post-fixation). While shrinkage after tissue removal was found in samples from the thorax, abdomen, and rear leg in dogs and from the rear leg in cats, no significant shrinkage due to formalin fixation was detected in any sample except for the thoracic samples from the dog. Therefore, when determining where to make incisions to effect a surgical cure, initial measurements should take into account tissue shrinkage effects.

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Evaluation of surgical margins required for complete excision of cutaneous mast cell tumors in dogs

Abstract: Results suggest that a 2-cm lateral margin and a deep margin of 1 fascial plane appear to be adequate for complete excision of grade-I and -II MCTs in dogs.

Cited by 132 publications

References 9 publications

Tomografia computadorizada de mastocitomas em cães: avaliação pré e pós-tratamento quimioterápico

Tomografia computadorizada de mastocitomas em cães: avaliação pré e pós-tratamento quimioterápico

c-KIT messenger RNA and protein expression and mutations in canine cutaneous mast cell tumors

Evaluation of the effect of formalin fixation on skin specimens in dogs and cats

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