Evaluation of susceptibility of an olive progeny (Picholine x Meski) to olive leaf spot disease caused by Fusicladium oleagineum

Rhouma, Ali; Chettaoui, M.; Krid, S.; Elbsir, H.; Msallem, M.; Triki, Mohamed Ali

doi:10.1007/s10658-012-0062-x

Cited by 10 publications

(6 citation statements)

References 27 publications

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“…Reports indicated that trichome can defence against bacterial pathogens by several means such as reducing and/or hindering the water film formation on the leaf surface, making difficult to germinate spore and subsequent bacterial multiplication (Nicks & Rubiales 2002;Medeiros et al 2004;Kang et al 2010). Moreover, Rhouma et al (2013) reported that resistant olive hybrids were characterised by a high number of trichomes in comparison with susceptible cultivars for olive leaf spot disease.…”

Section: Discussionmentioning

confidence: 99%

Association of morphological and biochemical features of mulberry lines with resistance to bacterial leaf spot

Banerjee

Chattopadhyay

Saha

et al. 2014

Archives Of Phytopathology And Plant Protection

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Xanthomonas campestris pv. mori causes bacterial leaf spot (BLS) of mulberry (Morus spp.) worldwide. Twenty mulberry lines with contrasting response to BLS showed significant (p < 0.01) variation between resistant and susceptible groups for stomata frequency (SF), trichome density (TD), total soluble sugar (TSS) and polyphenol (PP) content, while non-significant for chlorophyll content. Heritability (broad-sense) estimates obtained from the variance component analysis were high (≥0.72) for these traits. The disease severity index values of the lines were significantly negatively correlated with TD (r = −0.66; p < 0.01), TSS (r = −0.73; p < 0.01) and PP (r = −0.61; p < 0.01), while positively correlated with SF (r = 0.65; p < 0.01). The cluster analysis was based on highly significant four variables and DSI values, and classified the lines into two major clusters. The resistant lines that come under cluster-I had high TD, TSS and PP, while those in susceptible category had high SF in cluster-II. These findings suggest that direct selection for SF, TD, TSS and PP will be effective for development of host resistance against BLS in mulberry.

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Section: Discussionmentioning

confidence: 99%

Association of morphological and biochemical features of mulberry lines with resistance to bacterial leaf spot

Banerjee

Chattopadhyay

Saha

et al. 2014

Archives Of Phytopathology And Plant Protection

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“…Other biological and metabolic barriers could also prevent the successful extraction of thylakoids in plant species characterized by very thick and leathery leaves such as conifers, palm trees, or olive trees ( Olea europaea ) [ 37 ], which exhibit an elevated wax content in the cuticle. Nevertheless, the optimized protocol works well on the various plant species tested in this study, particularly Posidonia oceanica , but also Vitis vinifera and Quercus pubescens , which are very rich in polyphenols.…”

Section: Discussionmentioning

confidence: 99%

An efficient protocol for extracting thylakoid membranes and total leaf proteins from Posidonia oceanica and other polyphenol-rich plants

Charras,

Rey,

Guillemain

et al. 2024

Plant Methods

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Background The extraction of thylakoids is an essential step in studying the structure of photosynthetic complexes and several other aspects of the photosynthetic process in plants. Conventional protocols have been developed for selected land plants grown in controlled conditions. Plants accumulate defensive chemical compounds such as polyphenols to cope with environmental stresses. When the polyphenol levels are high, their oxidation and cross-linking properties prevent thylakoid extraction. Results In this study, we developed a method to counteract the hindering effects of polyphenols by modifying the grinding buffer with the addition of both vitamin C (VitC) and polyethylene glycol (PEG4000). This protocol was first applied to the marine plant Posidonia oceanica and then extended to other plants synthesizing substantial amounts of polyphenols, such as Quercus pubescens (oak) and Vitis vinifera (grapevine). Native gel analysis showed that photosynthetic complexes (PSII, PSI, and LHCII) can be extracted from purified membranes and fractionated comparably to those extracted from the model plant Arabidopsis thaliana. Moreover, total protein extraction from frozen P. oceanica leaves was also efficiently carried out using a denaturing buffer containing PEG and VitC. Conclusions Our work shows that the use of PEG and VitC significantly improves the isolation of native thylakoids, native photosynthetic complexes, and total proteins from plants containing high amounts of polyphenols and thus enables studies on photosynthesis in various plant species grown in natural conditions.

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“…The resistance of olive to OLS has been associated with both physical and chemical factors. Among the physical factors, trichome density and cuticle thickness have been recognized as the most important [ 40 ], while among chemical parameters, phenolic compounds are those determining the resistance of olive to OLS [ 41 ]. Olive leaf and olive fruit extracts are dominated by a vast variety of phenolic compounds, such as oleuropeine, rutin, tyrosol and others, with some of them exhibiting strong antifungal properties [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Laminarin Induces Defense Responses and Efficiently Controls Olive Leaf Spot Disease in Olive

2021

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Olive leaf spot (OLS) caused by Fusicladiumoleagineum is mainly controlled using copper fungicides. However, the replacement of copper-based products with eco-friendly alternatives is a priority. The use of plant resistance-inducers (PRIs) or biological control agents (BCAs) could contribute in this direction. In this study we investigated the potential use of three PRIs (laminarin, acibenzolar-S-methyl, harpin) and a BCA (Bacillus amyloliquefaciens FZB24) for the management of OLS. The tested products provided control efficacy higher than 68%. In most cases, dual applications provided higher (p < 0.05) control efficacies compared to that achieved by single applications. The highest control efficacy of 100% was achieved by laminarin. Expression analysis of the selected genes by RT-qPCR revealed different kinetics of induction. In laminarin-treated plants, for most of the tested genes a higher induction rate (p < 0.05) was observed at 3 days post application. Pal, Lox, Cuao and Mpol were the genes with the higher inductions in laminarin-treated and artificially inoculated plants. The results of this study are expected to contribute towards a better understanding of PRIs in olive culture and the optimization of OLS control, while they provide evidence for potential contributions in the reduction of copper accumulation in the environment.

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Evaluation of susceptibility of an olive progeny (Picholine x Meski) to olive leaf spot disease caused by Fusicladium oleagineum

Abstract: Forty five descendants from a crossing between olive cultivar Picholine de Languedoc and the local cultivar Meski were evaluated for their susceptibility to olive leaf spot (Fusicladium oleagineum) in field and laboratory studies.

Cited by 10 publications

References 27 publications

Association of morphological and biochemical features of mulberry lines with resistance to bacterial leaf spot

Association of morphological and biochemical features of mulberry lines with resistance to bacterial leaf spot

An efficient protocol for extracting thylakoid membranes and total leaf proteins from Posidonia oceanica and other polyphenol-rich plants

Laminarin Induces Defense Responses and Efficiently Controls Olive Leaf Spot Disease in Olive

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