Evaluation of technological properties of Enterococcus faecium CECT 8849, a strain isolated from human milk, for the dairy industry

Cárdenas, Nívia; Arroyo, Rebeca; Calzada, Javier; Peirotén, Ángela; Médina, Margarita; Rodríguez, Juan M.; Fernández, Leónides

doi:10.1007/s00253-016-7616-3

Cited by 10 publications

(6 citation statements)

References 61 publications

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“…The human milk microbiota may contribute to, at least, some of the functional properties and health benefits that epidemiological studies have associated with breastfeeding (Renfrew et al, 2012), including protection against infections, metabolic programming, immunomodulation and neuromodulation. Human milk bacteria may provide a certain degree of protection against infections caused by viruses, bacteria or fungi through a variety of mechanisms: (a) biosynthesis of compounds with antimicrobial activity, including organic acids (lactic acid, acetic acid, ethanol), bacteriocins, reuterin or hydrogen peroxide (Heikkilä and Saris, 2003;Beasley and Saris, 2004;Martıń et al, 2005;Martıń et al, 2006;Caŕdenas et al, 2016;Caŕdenas et al, 2019;Angelopoulou et al, 2020;Garcıá-Gutierrez et al, 2020); (b) coaggregation with pathobionts, impeding their access to the gut epithelial cells (Caŕdenas et al, 2019); (c) competitive exclusion with pathobionts for nutrients or host receptors (Olivares et al, 2006a;Martıń et al, 2010;Langa et al, 2012); (d) reinforcement of the infant gut barrier by preserving and decreasing intestinal permeability and increasing mucin biosynthesis (Olivares et al, 2006a;Vanhaecke et al, 2017;Liu et al, 2020); and (e) inmmunomodulation (Liu et al, 2020).…”

Section: Moving From Composition To Functionmentioning

confidence: 99%

The Microbiota of the Human Mammary Ecosystem

Fernández

Pannaraj

Rautava

et al. 2020

Front. Cell. Infect. Microbiol.

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Human milk contains a dynamic and complex site-specific microbiome, which is not assembled in an aleatory way, formed by organized microbial consortia and networks. Presence of some genera, such as Staphylococcus, Streptococcus, Corynebacterium, Cutibacterium (formerly known as Propionibacterium), Lactobacillus, Lactococcus and Bifidobacterium, has been detected by both culture-dependent and culture-independent approaches. DNA from some gut-associated strict anaerobes has also been repeatedly found and some studies have revealed the presence of cells and/or nucleic acids from viruses, archaea, fungi and protozoa in human milk. Colostrum and milk microbes are transmitted to the infant and, therefore, they are among the first colonizers of the human gut. Still, the significance of human milk microbes in infant gut colonization remains an open question. Clinical studies trying to elucidate the question are confounded by the profound impact of non-microbial human milk components to intestinal microecology. Modifications in the microbiota of human milk may have biological consequences for infant colonization, metabolism, immune and neuroendocrine development, and for mammary health. However, the factors driving differences in the composition of the human milk microbiome remain poorly known. In addition to colostrum and milk, breast tissue in lactating and non-lactating women may also contain a microbiota, with implications in the pathogenesis of breast cancer and in some of the adverse outcomes associated with breast implants. This and other open issues, such as the origin of the human milk microbiome, and the current limitations and future prospects are addressed in this review.

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Section: Moving From Composition To Functionmentioning

confidence: 99%

The Microbiota of the Human Mammary Ecosystem

Fernández

Pannaraj

Rautava

et al. 2020

Front. Cell. Infect. Microbiol.

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“…The L-15 extract promoted chondrogenic differentiation by increasing certain extracellular matrix components (e.g., GAG). E. faecium is a type of LAB present in the human gastrointestinal tract and commonly used to produce fermented meat or dairy products [35,36]. Several groups have used LAB to induce adipogenic or osteogenic differentiation [37,38].…”

Section: Discussionmentioning

confidence: 99%

Enterococcus faecium L-15 Cell-Free Extract Improves the Chondrogenic Differentiation of Human Dental Pulp Stem Cells

Kim

Park

Kim

et al. 2019

IJMS

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Hyaline cartilage is a tissue of very low regenerative capacity because of its histology and limited nutrient supply. Cell-based therapies have been spotlighted in the regeneration of damaged cartilage. Dental pulp stem cells (DPSCs) are multipotent and are easily accessible for therapeutic purposes. In human gastrointestinal tracts, Enterococcus faecium is a naturally occurring commensal species of lactic acid bacteria. In this work, the human DPSCs were differentiated into chondrocytes using a chondrogenic differentiation medium with or without L-15 extract. We observed that chondrogenic differentiation improved in an E. faecium L-15 extract (L-15)-treated DPSC group via evaluation of chondrogenic-marker mRNA expression levels. In particular, we found that L-15 treatment promoted early-stage DPSC differentiation. Cells treated with L-15 were inhibited at later stages and were less likely to transform into hypertrophic chondrocytes. In L-15-treated groups, the total amount of cartilage extracellular matrix increased during the differentiation process. These results suggest that L-15 promotes chondrogenic differentiation, and that L-15 may be used for cartilage repair or cartilage health supplements. To our knowledge, this is the first report demonstrating the beneficial effect of L-15 treatment on chondrogenic differentiation.

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“…It was established that enterococci produce numerous volatile compounds and contribute to the formation of the cheese aroma, especially during ripening [17,91,100,138]. In the study of Cárdenas et al [139], a total of 41 volatile compounds were identified in experimental cheeses manufactured with E. faecium CECT 8849.…”

Section: Production Of Aromatic Compoundsmentioning

confidence: 99%

Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns

Terzić-Vidojević

Veljović

Popović

et al. 2021

Foods

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The present study is focused on the safety, technological characteristics, and probiotic evaluation of Enterococcus species from different artisanal raw milk dairy products, mainly cheeses with ripening. Apart from proteolytic and lipolytic activities, most enterococci show the ability to metabolize citrate and convert it to various aromatic compounds. Long-ripened cheeses therefore have a specific flavor that makes them different from cheeses produced from thermally treated milk with commercial starter cultures. In addition, enterococci are producers of bacteriocins effective against spoilage and pathogenic bacteria, so they can be used as food preservatives. However, the use of enterococci in the dairy industry should be approached with caution. Although originating from food, enterococci strains may carry various virulence factors and antibiotic-resistance genes and can have many adverse effects on human health. Still, despite their controversial status, the use of enterococci in the food industry is not strictly regulated since the existence of these so-called desirable and undesirable traits in enterococci is a strain-dependent characteristic. To be specific, the results of many studies showed that there are some enterococci strains that are safe for use as starter cultures or as probiotics since they do not carry virulence factors and antibiotic-resistance genes. These strains even exhibit strong health-promoting effects such as stimulation of the immune response, anti-inflammatory activity, hypocholesterolemic action, and usefulness in prevention/treatment of some diseases.

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Evaluation of technological properties of Enterococcus faecium CECT 8849, a strain isolated from human milk, for the dairy industry

Cited by 10 publications

References 61 publications

The Microbiota of the Human Mammary Ecosystem

The Microbiota of the Human Mammary Ecosystem

Enterococcus faecium L-15 Cell-Free Extract Improves the Chondrogenic Differentiation of Human Dental Pulp Stem Cells

Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns

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