Evaluation of Teflon-coated intraocular lenses in an organ culture method

Abstract

An amorphous and transparent form of Teflon is proposed as a coating of polymethylmethacrylate (PMMA) intraocular lenses (IOLs), rendering them highly hydrophobic. We used an organ culture method to evaluate cell adhesion, proliferation, and migration on Teflon-coated IOLs. Corneal explants from 14-day-old chicken embryos were placed on a semisolid culture medium and covered with uncoated PMMA (n = 36) and Teflon-coated PMMA (n = 36) IOLs and two controls, Thermanox (n = 84) and latex (n = 36). After incubation (7 days at 37 degrees C), a digital imaging system was used to measure the areas of the cell migration layers on the materials. The cells were then removed with tripsin-ethylenediaminetetraacetic acid and the cells detached at times up to 75 min were counted (Coulter(R) Multisizer System). The values were used to construct a cell disconnecting curve for each material. The areas of cell migration layers on uncoated and Teflon-coated IOLs were significantly different (p <.05). Cell disconnecting curves demonstrated that cells adhered less strongly to Teflon-coated IOLs than to the other materials. This organ culture method demonstrated that the coating of PMMA IOLs with Teflon AF(R) is correlated with antiadhesive and antiproliferative properties.

Polymer coating of paramagnetic particulates for in vivo oxygen-sensing applications

“…Accordingly, TAF is widely used in gas sensing and filtration systems, and is biocompatible (used in contact lens, Werner et al 1999). Cellulose is the most abundant naturally occurring polysaccharide, formed out of glucose-based repeat units, connected by 1,4-beta-glucosidic linkages.…”

Selective control of liver and kidney cells migration during organotypic cocultures inside fibronectin-coated rectangular silicone microchannels

“…The method consists in working with excised tissue samples from embryonic explants and adult liver slices [25][26][27][28][29][30]. Using the technique of embryonic organotypic culture, microchannels can be used to physically orient the liver cell migration and the liver expansion along the length direction of the microchannels.…”

Electrowetting on plasma-deposited fluorocarbon hydrophobic films for biofluid transport in microfluidics

“…Examples of such applications include real-time principle component regression, 30 biochemical assays for various biomolecules detection, 31,32 and get as sophisticated as to allow analysis of peptides and proteins 33 by matrix-assisted laser desorption/ionization mass spectrometry. Teflon ® -like films are known for their chemi-cal inertness, 34,35 biocompatibility, 36,37 and nonadsorbability, 38 thus they are suitable for bioapplications. A way to avoid biomolecule adsorption is to encapsulate the liquid droplet to be transported in immiscible liquids ͑such as silicone oil͒ to prevent contact with the device surfaces.…”