Evaluation of Temporary Immersion System for in Vitro Propagation of Stevia Rebaudiana Bertoni

Sacco, E.; Mascarello, C.; Pamato, M.; Musso, V.; Ruffoni, B.

doi:10.17660/actahortic.2015.1083.41

Cited by 5 publications

(3 citation statements)

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“…5 indicated the feasibility of culturing chestnut shoots Plant Cell Tiss Organ Cult both in RITA Ò and in plantform TM bioreactors, although basal explants cultured in plantform TM bioreactors yielded better results than RITA Ò vessels in three of the five clones tested in terms multiplication ability, and in the five clones in terms of shoot length. Sacco et al (2015), also compared the performance of RITA Ò and plantform TM in the micropropagation of Stevia rebaudiana (sweetleaf). In this species, RITA Ò vessels yielded more shoots of good quality than the plantform TM bioreactors, although the hyperhydricity observed in the latter type of container could be avoided with longer intervals between immersion.…”

Section: Resultsmentioning

confidence: 99%

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Vidal

Blanco

Cuenca

2015

Plant Cell Tiss Organ Cult

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A protocol for culturing chestnut axillary shoots by temporary immersion in liquid medium was developed. The influence of type of explant, support material, bioreactor, and immersion was investigated for five artificial hybrids and five natural hybrids of Asian and European chestnut selected for resistance to ink disease. The type of explant influenced shoot quality and proliferation rates, and basal explants with callus produced more and longer shoots than apical and nodal segments. Use of rockwool cubes as support material prevented hyperhydricity and allowed proliferation of explants in Murashige and Skoog medium with half-strength nitrates supplemented with 0.22 lM BA and 3 % sucrose, cultured both in plantform TM and RITA Ò vessels with three or six immersions per day and additional aeration of 1 min per hour in the case of plantform TM bioreactors. Basal explants cultured in plantform TM for 5 weeks produced long shoots suitable for rooting, whereas apical and nodal explants cultured in plantform TM or RITA Ò produced shorter shoots that were suitable for maintenance of stock. For most of the clones, similar or higher proliferation rates were observed when cultured in liquid medium than in semisolid medium, with the additional benefit of cost-reduction of the former system. Shoots developed in liquid medium were submitted to ex vitro root induction by dipping in indole-3-butyric acid, and acclimatized under greenhouse conditions. This is the first demonstration of the production of chestnut plantlets from shoots cultured in liquid medium, and the protocol presented here shows good potential for application in large-scale propagation.

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Section: Resultsmentioning

confidence: 99%

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Vidal

Blanco

Cuenca

2015

Plant Cell Tiss Organ Cult

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“…These containers have also given the best results in the micropropagation of chestnut (Vidal et al 2015), and in Phoenix dactylifera (Nayyef et al 2019). On the other hand, in the micropropagation of Stevia rebaudiana, Sacco et al (2015) obtained better results, more shoots, and better quality with RITA® vessels.…”

Section: Shoot Numbermentioning

confidence: 97%

Temporary immersion systems to improve alder micropropagation

José

Blázquez

Cernadas

et al. 2020

Plant Cell Tiss Organ Cult

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A protocol has been developed for the propagation of Alnus glutinosa axillary shoots in liquid medium. The explants were cultured in Woody Plant Medium supplemented with 0.1 mg l − 1 benzyladenine and 0.5 mg l − 1 indole acetic acid. The effect of the bioreactor type (RITA® and Plantform™), frequency of the immersions, regulator concentrations, and volume of medium per explant was investigated. All the treatments with the temporal immersion systems (TIS) increased the proliferation rates. The best results were obtained on using the Plantform™ vessels and, unlike the shoots cultured in RITA® vessels, the culture in Plantform™ had very low hyperhydricity percentages. Shoots originating from the culture in semisolid medium and in Plantform™ rooted in semi-solid medium with 0.1 mg/l indole butyric acid for 7 days. No significant differences were observed in the rooting or acclimatization percentages, with survival percentages greater than 85% being achieved. This is the first work on the use of TIS systems in alder propagation, with the results of this study providing new perspectives for its mass propagation. Key messageA new protocol is described in order to reduce the propagation costs of alder.

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“…The liquid culture in PlantForm or RITA bioreactors was found to be efficient for Stevia rebaudiana propagation, with the number of shoots per explant equal to 14, as well as for its rooting phase [94]. The authors noted that, although the PlantForm has a larger space to allow for material enlargement rather than RITA, it is necessary to carefully set the flooding conditions with the PlantForm system to obtain good quality plants suitable for the acclimatization phase in greenhouse; flooding every 8 h guaranteed a better behavior and quality without hyperhydricity.…”

Section: Types Of Tis Bioreactors and Biomass Productionmentioning

confidence: 99%

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

et al. 2021

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The cultivation of medicinal plants and the production of bioactive compounds derived from them are of fundamental importance and interest, not only at the pharmacological level but also in nutraceutical and cosmetic industries and in functional foods, as well as plant protection in agriculture. In order to respond adequately to the increased demands of the global market from a quantitative and qualitative point of view and to guarantee environmental sustainability of the productions, it is necessary to resort to innovation tools, such as tissue culture in vitro technology. Nowadays, it is well known that the cultivation through the Temporary Immersion System (TIS) in a bioreactor has considerable advantages both for the in vitro mass production of the plants and for the production of secondary metabolites. The present review focuses on the application of TIS during the last two decades to produce biomass and bioactive compounds from medicinal plants. Indeed, almost one hundred papers are discussed, and they particularly focus on the effects of the culture system, vessel design and equipment, immersion time and frequency, and substrate composition for 88 medicinal species in TIS bioreactor culture.

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Evaluation of Temporary Immersion System for in Vitro Propagation of Stevia Rebaudiana Bertoni

Cited by 5 publications

References 0 publications

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Temporary immersion systems to improve alder micropropagation

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

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