Evaluation of the ability of commercial disinfectants to degrade free nucleic acid commonly targeted using molecular diagnostics

Stoufer, Sloane; Demokritou, Melina; Buckley, D.; Teska, Peter J.; Moore, Matthew D.

doi:10.1016/j.jhin.2022.12.010

Cited by 5 publications

(1 citation statement)

References 14 publications

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“…High concentration of free nucleic acids can dilute the target pathogen's genetic material within a sample, reducing the sensitivity of detection methods and making it more difficult to identify low pathogen levels in wastewater. Moreover, free nucleic acids can act as potential sources of cross-contamination in laboratories, leading to inaccurate results [21].…”

Section: Free Nucleic Acids In Wastewater Samplesmentioning

confidence: 99%

Advances in Pretreatment Methods for Free Nucleic Acid Removal in Wastewater Samples: Enhancing Accuracy in Pathogenic Detection and Future Directions

Vu,

Nguyen,

Nguyen

2023

Preprint

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Accurate pathogenic detection in wastewater is critical for safeguarding public health and the environment. However, the presence of free nucleic acids in wastewater samples poses significant challenges to molecular detection accuracy. This comprehensive review explores the current status and future potential of pretreatment methods to remove free nucleic acids from wastewater samples. The study contributes a comprehensive analysis of the mechanisms, strengths, and limitations of various pretreatment approaches, including physical, chemical, and enzymatic processes. The effect of various factors on the removal efficiency of these pretreatment methods is also discussed. This review enhances our comprehension of pretreatment techniques and their vital role in achieving precise pathogenic detection in complex wastewater matrices. Furthermore, it outlines future perspectives and developments for improving the speed and effectiveness of pathogenic detection, contributing significantly to disease surveillance, early warning systems, and environmental protection.

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Section: Free Nucleic Acids In Wastewater Samplesmentioning

confidence: 99%

Advances in Pretreatment Methods for Free Nucleic Acid Removal in Wastewater Samples: Enhancing Accuracy in Pathogenic Detection and Future Directions

Vu,

Nguyen,

Nguyen

2023

Preprint

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Environmental DNA for monitoring the impact of offshore wind farms on fish and invertebrate community structures

Cornelis,

De Backer,

Maes

et al. 2024

Environmental DNA

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To reach the renewable energy targets set by the European Commission, a tenfold expansion of the installed offshore wind farms is needed. Since the construction of offshore wind farms may affect local soft‐sediment fauna, an efficient monitoring technique is needed to monitor the potential effects on the marine ecosystem. Here, we assess whether eDNA metabarcoding is a suitable alternative to monitor fish and epibenthos biodiversity in these difficult to access marine habitats. Water sampling and trawl surveys were conducted in parallel in 12 coastal and 18 offshore sites, the latter located inside and outside two offshore wind farms in the Belgian part of the North Sea. 12S eDNA metabarcoding retrieved 85.7% of the fish species caught in the beam trawls, whereas the COI eDNA metabarcoding only identified 31.4% of the epibenthic invertebrate species. Furthermore, the 12S marker resulted in an additional detection of 26 unique fish species, whereas the COI marker detected an additional 90 invertebrate species. Spatial patterns in alpha diversity recovered with eDNA metabarcoding were not significantly different from those observed with morphological determination. Significant differences were found in fish and invertebrate community structures between the coastal, transition and offshore zones as well as on the smaller wind farm scales, which agreed with the morphological beam trawl data. Indicator species found with morphological beam trawl monitoring for each of the three zones (coastal, transition, offshore) were also detected with 12S eDNA metabarcoding, and the latter method detected an additional 31 indicator species. Our findings show the need for adequate quality control of the obtained species lists and reveal that 12S eDNA metabarcoding analyses offers a useful survey tool for the monitoring of fish communities in offshore wind farms, but the used COI assay did not adequately capture the epibenthic communities as observed with beam trawl data.

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Analysis of Cytokine-Inducible SH2-Containing Protein (CISH) in the Study of Immunosenescence

Yin,

Jin

2024

Methods in Molecular Biology

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Evaluation of the ability of commercial disinfectants to degrade free nucleic acid commonly targeted using molecular diagnostics

Cited by 5 publications

References 14 publications

Advances in Pretreatment Methods for Free Nucleic Acid Removal in Wastewater Samples: Enhancing Accuracy in Pathogenic Detection and Future Directions

Advances in Pretreatment Methods for Free Nucleic Acid Removal in Wastewater Samples: Enhancing Accuracy in Pathogenic Detection and Future Directions

Environmental DNA for monitoring the impact of offshore wind farms on fish and invertebrate community structures

Analysis of Cytokine-Inducible SH2-Containing Protein (CISH) in the Study of Immunosenescence

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