Evaluation of the Absorption of Methionine Carried by Mineral Clays and Zeolites in Porcine Ex Vivo Permeability Models

Giromini, Carlotta; Tretola, M.; Cristiani, Cinzia; Finocchio, Elisabetta; Silacci, Paolo; Panseri, Sara; Dell’Anno, Matteo; Baldi, Alfonso; Rossi, Luciana

doi:10.3390/app11146384

Cited by 3 publications

(5 citation statements)

References 28 publications

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“…Our synthetic approach-solid/liquid adsorption-is known to be effective in preparing organoclays [31,32,56], with the right features as those required for animal nutrition, such as well-dispersed LY loadings and reversible interactions with the carrier. The final result depends on the synthesis parameters [30][31][32]; i.e., the biomolecule concentration, pH, and reaction time, as well as the carrier characteristics, morphology, and the zero point charge.…”

Section: Discussionmentioning

confidence: 99%

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A Commercial Clay-Based Material as a Carrier for Targeted Lysozyme Delivery in Animal Feed

Guagliano,

Cristiani,

Dell’Anno

et al. 2023

Nanomaterials

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The controlled supply of bioactive molecules is a subject of debate in animal nutrition. The release of bioactive molecules in the target organ, in this case the intestine, results in improved feed, as well as having a lower environmental impact. However, the degradation of bioactive molecules’ in transit in the gastrointestinal passage is still an unresolved issue. This paper discusses the feasibility of a simple and cost-effective procedure to bypass the degradation problem. A solid/liquid adsorption procedure was applied, and the operating parameters (pH, reaction time, and LY initial concentration) were studied. Lysozyme is used in this work as a representative bioactive molecule, while Adsorbo®, a commercial mixture of clay minerals and zeolites which meets current feed regulations, is used as the carrier. A maximum LY loading of 32 mgLY/gAD (LY(32)-AD) was obtained, with fixing pH in the range 7.5–8, initial LY content at 37.5 mgLY/gAD, and reaction time at 30 min. A full characterisation of the hybrid organoclay highlighted that LY molecules were homogeneously spread on the carrier’s surface, where the LY–carrier interaction was mainly due to charge interaction. Preliminary release tests performed on the LY(32)-AD synthesised sample showed a higher releasing capacity, raising the pH from 3 to 7. In addition, a preliminary Trolox equivalent antioxidant capacity (TEAC) assay showed an antioxidant capacity for the LY of 1.47 ± 0.18 µmol TroloxEq/g with an inhibition percentage of 33.20 ± 3.94%.

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Section: Discussionmentioning

confidence: 99%

“…Among others, the binding of the bioactive molecule to a suitable carrier via an adsorption process has been proposed and would seem to be a viable, simple, and costeffective solution [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

A Commercial Clay-Based Material as a Carrier for Targeted Lysozyme Delivery in Animal Feed

Guagliano,

Cristiani,

Dell’Anno

et al. 2023

Nanomaterials

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“…The FT-IR spectrum of the MBIB ligand showed a change in the position of OH band compared with the original BIB ligand because of the insertion of methionine moiety (Table 3) [11]. A new band appears in the ligand and complex spectra at 1612-1622 cm -1 , which is assigned to the C=O group [12].…”

Section: Ft-ir Spectroscopymentioning

confidence: 98%

“…All the details of the spectra are listed in Table 5. The spectrum of MBIB (Figure 10) exhibited the bands in the region of 323 nm (30959 cm -1 ), which were assigned to the π -π* transition [11]. The spectrum of the Ni(II) complex (Figure 11) exhibited a shift in the position of the π -π* transition.…”

Section: Uv-vis Spectral Studiesmentioning

confidence: 99%

Synthesis, Identification and Biological Study of New Pharmaceutical Model Based on Amino Acids with Some of Its Complexes

Mahmoud,

Khaleel

2023

Iraqi Journal of Science

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The synthesis of the MBIB ligand by the reaction of the BIB ligand with methionine in 1:1 ratio, and the metal complexes with Ni(II), Cu(II), and Pt(IV) were described. All synthesized compounds were characterized using spectroscopic methods such as FT-IR, 1H NMR, UV-VIS, thermal analysis (TG and DSC), atomic absorption (AAS), elemental microanalysis (C.H.N.S), melting point (m.p.), magnetic susceptibility, molar conductivity measurements, and chloride content. All the complexes were electrolytes, and the suggested geometric shapes for the complexes were octahedral. The magnetic properties of the platinum complex were diamagnetic, while those of the nickel and copper complexes were paramagnetic. All synthesized compounds have good anti-biofilm properties against bacteria (Pseudomonas auroginosa, a gram-negative bacteria), except for C1(Ni), which is inactive against the same bacteria. In addition, the ligand was evaluated as an anti-cancer agent against human breast cancer (MCF-7), but its effectiveness has been shown to be less effective compared to metronidazole.

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“…Accordingly, hybrid materials, consisting of the lysozyme bonded to the inorganic carriers, have been prepared via a solid/liquid adsorption reaction in aqueous environment. This process has already been reported to be easy, environmentally friendly, and cost effective [7,22].…”

Section: Introductionmentioning

confidence: 95%

Lysozyme–Mineral Clay Systems: Comparison of Interaction for Controlled Release in Feed Application

Guagliano,

Dell’Anno,

Dotelli

et al. 2023

Minerals

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A synthetic approach to bond lysozyme (LY) to commercial natural carriers, namely clay minerals (bentonite, BN; and sepiolite, SP) and commercial zeolite (Phil 75®, PH), already in use in feed formulation, is proposed. The synthetic route, which implies solid–liquid adsorption, is a simple and effective way for preparing hybrid materials characterized by LY loadings up to 37 mgLY/gcarrier. By operating at pH 4.3, initial LY content of 37.5 mgLY/gcarrier, and reaction time of 90 min, hybrid materials with LY loadings of 37, 35, and 12 mgLY/gcarrier for LY-SP, LY-BN, and LY-PH, respectively were obtained. The LY initial concentration and pH, as well as the physico-chemical properties of the carries were found to be the parameters that govern the synthesis of the materials. The driving force for an effective LY adsorption and interaction is the combined Zero Point Charge (ZPC) of the carriers, always negative (in the range between −4 and −170 mV) and the positive ZPC of LY, as well as the carrier morphology, characterized by mesoporosity (pore dimensions in the range of 5–12 nm). However, it is the interaction of charges of opposite sign that mainly affects LY loadings and bond strength. Based on SEM-EDX analysis, LY molecules are quite homogeneously spread onto the carriers’ surface. TG-DTG analyses showed that the LY–carrier interaction in the hybrid materials is stronger than that in a simple mechanical mixture of the components. Specifically, in the hybrid materials, the phenomenon at 300 °C, associated to LY decomposition, is broadened and slightly shifted towards higher temperatures (320–350 °C), whereas in a mechanical mixture of the same composition, it occurs at temperatures closer to those of free LY, as if there were no or very weak interactions. At pH 3, a very little LY release, 0.03 and 0.01 mgLY/gcarrier, was found for LY-BN and LY-PH, respectively. The latter became larger at pH 7, 0.06 mgLY/gcarrier for both BN and PH carriers, suggesting that BN and PH are better modulators of LY release. The paper provides insights for the study and the development of new optimized feed formulations for the targeted delivery of natural compounds with antimicrobial activity, alternatives to antibiotics, and vaccinal antigens.

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Evaluation of the Absorption of Methionine Carried by Mineral Clays and Zeolites in Porcine Ex Vivo Permeability Models

Cited by 3 publications

References 28 publications

A Commercial Clay-Based Material as a Carrier for Targeted Lysozyme Delivery in Animal Feed

A Commercial Clay-Based Material as a Carrier for Targeted Lysozyme Delivery in Animal Feed

Synthesis, Identification and Biological Study of New Pharmaceutical Model Based on Amino Acids with Some of Its Complexes

Lysozyme–Mineral Clay Systems: Comparison of Interaction for Controlled Release in Feed Application

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