Evaluation of the Agilent Technologies Bioanalyzer-based DNA Fish Identification Solution

Handy, Sara M.; Mueller, Steffen; Jacob, Salena M.; Paul, Stephen Z.; Garrett, S. D.; Deeds, Jonathan R.

doi:10.1016/j.foodcont.2016.09.013

Cited by 3 publications

(3 citation statements)

References 9 publications

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“…Briefly, 1 µL PCR product and 5 µL markers were loaded into each of the 12 wells, and the gel-dye mixture was applied to the chip run in the bioanalyzer. For the Agilent DNA, the 1000 chip peaks are ±5% from 100 to 500 bp, the sizing accuracy is 10%, and the sizing reproducibility is 5% coefficient of variation (CV) per the specifications [22]. The information on the base sequence and primers in the identification solution are listed in Table 1.…”

Section: Dna-traceable Barcodes Encapsulation and Post-recycling Iden...mentioning

confidence: 99%

An Accurate and Rapid Way for Identifying Food Geographical Origin and Authenticity: Editable DNA-Traceable Barcode

Liu

Xing

Sun

et al. 2022

Foods

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DNA offers significant advantages in information density, durability, and replication efficiency compared with information labeling solutions using electronic, magnetic, or optical devices. Synthetic DNA containing specific information via gene editing techniques is a promising identifying approach. We developed a new traceability approach to convert traditional digitized information into DNA sequence information. We used encapsulation to make it stable for storage and to enable reading and detection by DNA sequencing and PCR-capillary electrophoresis (PCR-CE). The synthesized fragment consisted of a short fragment of the mitochondrial cytochrome oxidase subunit I (COI) gene from the Holothuria fuscogilva (ID: LC593268.1), inserted geographical origin information (18 bp), and authenticity information from Citrus sinensis (20 bp). The obtained DNA-traceable barcodes were cloned into vector PMD19-T. Sanger sequencing of the DNA-traceable barcode vector was 100% accurate and provided a complete readout of the traceability information. Using selected recognition primers CAI-B, DNA-traceable barcodes were identified rapidly by PCR amplification. We encapsulated the DNA-traceable barcodes into amorphous silica spheres and improved the encapsulation procedure to ensure the durability of the DNA-traceable barcodes. To demonstrate the applicability of DNA-traceable barcodes as product labels, we selected Citrus sinensis as an example. We found that the recovered and purified DNA-traceable barcode can be analyzed by standard techniques (PCR-CE for DNA-traceable barcode identification and DNA sequencing for readout). This study provides an accurate and rapid approach to identifying and certifying products’ authenticity and traceability.

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Section: Dna-traceable Barcodes Encapsulation and Post-recycling Iden...mentioning

confidence: 99%

An Accurate and Rapid Way for Identifying Food Geographical Origin and Authenticity: Editable DNA-Traceable Barcode

Liu

Xing

Sun

et al. 2022

Foods

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“…Barcoding is a worldwide project where hundreds of laboratories have been involved in enlisting the global biodiversity. Accordingly, new methodologies of DNA extraction and Polymerase Chain Reaction (PCR) (Handy et al 2016) has been increasingly developed in parallel with the development of barcoding primers. To standardize the handling of DNA barcoding samples, BOLD has already made standard laboratory procedure (SLP) (Keele et al 2013).…”

Section: Dna Barcoding As An Efficient Tool For Species Identificationmentioning

confidence: 99%

“…In SLP (Fig 1) preferably fresh few millimetersized tissue is placed in 1.5mL Eppendorf tube. Fish Barcoding Method (Handy et al 2016) which is also available online at BOLD website (http: //www.dnabarcoding101.org/files/using-dna-barcodes.pdf) does DNA extraction. Species-specific primer or universal DNA barcoding primers (based on animals group i.e.…”

Section: Dna Barcoding As An Efficient Tool For Species Identificationmentioning

confidence: 99%

Review: Progress and potential of DNA barcoding for species identification of fish species

2017

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Imtiaz A, Mohd Nor SA, Md. Naim D. 2017. Review: Progress and potential of DNA barcoding for species identification of fish species. Biodiversitas 18: 1394-1405. DNA barcoding is a molecular technique to identify species by utilizing 600-800 base pairs genetic primer segments of mitochondrial gene cytochrome oxidase I. DNA barcoding has high potential to identify species into taxa, resolves ambiguousness in species identification, helps in accurate species identification, categorize species for conservation and also communize the information in the form of database system. The main challenge to this technique is regarding the use of barcoding information on â€˜biological species conceptâ€™. The extreme diversity of fish and their economic importance has made this group a major target of DNA barcoding. DNA barcoding can assign the status of known to unknown sample but it also has the ability to detect previously un-sampled species as distinct. In this review, we present an overview of DNA barcoding and introduce current advances and limitation of this promising technique.

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Nanoscience and DNA Barcoding

Pal

Banerjee

2020

Nanomaterials and Biomedicine

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Evaluation of the Agilent Technologies Bioanalyzer-based DNA Fish Identification Solution

Cited by 3 publications

References 9 publications

An Accurate and Rapid Way for Identifying Food Geographical Origin and Authenticity: Editable DNA-Traceable Barcode

An Accurate and Rapid Way for Identifying Food Geographical Origin and Authenticity: Editable DNA-Traceable Barcode

Review: Progress and potential of DNA barcoding for species identification of fish species

Nanoscience and DNA Barcoding

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