Evaluation of the Amplicor Chlamydia trachomatis test versus culture in genital samples in various prevalence populations.

Barbeyrac, Bertille de; Pellet, I; Dutilh, B; Bébéar, C.; Dumon, Béatrice; Géniaux, M

doi:10.1136/sti.70.3.162

Cited by 24 publications

(10 citation statements)

References 15 publications

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“…First, significantly more PCR-positive results were obtained with dry specimens than with swirled specimens when the specimens were first tested by PCR. The failure of 4 to 25% of the positive specimens to give positive PCR results when first tested has been reported for numerous previous studies (2,3,6,10,11,15). In the present study, falsenegative specimens which reverted to PCR positive when re- a None of the positive results for swirled specimens but one of the positive results for dry specimens reverted to negative when the initial PCR test was repeated.…”

supporting

confidence: 72%

Improved PCR detection of Chlamydia trachomatis by using an altered method of specimen transport and high-quality endocervical specimens

et al. 1995

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Duplicate endocervical swabs were collected for detection of Chlamydia trachomatis by PCR (Roche Diagnostics). One swab was swirled in Specimen Transport Medium (Roche) for PCR testing and discarded. A saline aliquot from the other specimen, sent as a dry swab to the laboratory, was Papanicolaou stained to determine specimen adequacy, and the remainder was PCR tested. Significantly more (24%) PCR-positive results (118 versus 95; P < 0.001) were obtained with the dry specimens than with the swirled specimens when first tested. In addition, PCR-positive results were obtained with 107 (10.6%) of 1,007 microscopically adequate specimens but with only 3 (0.9%) of 341 inadequate specimens (P < 0.001).

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supporting

confidence: 72%

Improved PCR detection of Chlamydia trachomatis by using an altered method of specimen transport and high-quality endocervical specimens

et al. 1995

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“…Mycoplasma were detected by culture in liquid urea‐arginine medium and passage on A7 medium for semiquantitative counting of Mycoplasma hominis and Ureaplasma urealyticum , after 48 h of anaerobic incubation. Chlamydia trachomatis was detected by PCR (Amplicor TM C. trachomatis , RMS) using urethral swabs [ 13]. Saprophytic organisms isolated from urethral swabs (Corynebacterium, Staphylococcus epidermidis , nonspecific group streptococci) were not considered pathogenic.…”

Section: Methodsmentioning

confidence: 99%

Developmental factors of urethral human papillomavirus lesions: correlation with circumcision

Aynaud¹,

Piron²,

Bijaoui³

et al. 1999

BJU International

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“…These assays have a lower detection limit of one to 10 elementary bodies and specificities comparable with culture. [8][9][10][11] They also oVer all the advantages of non-culture tests in terms of specimen transport, batching, and rapid processing time of approximately 4 hours and, in addition, automation for some assays. The improved sensitivity of these assays allows the use of non-invasive specimens such as first catch urine (FCU) specimens.…”

Section: Introductionmentioning

confidence: 99%

Pooling of urine specimens for PCR testing: a cost saving strategy for Chlamydia trachomatis control programmes

Peeling

Toye

Jessamine

et al. 1998

Sexually Transmitted Infections

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Objectives: To evaluate pooling of first catch urine (FCU) specimens as a cost eVective strategy for chlamydia testing. Methods: Mock specimens were pooled with and without dilution to determine optimal pool size and ease of work flow. The performance of the Amplicor Chlamydia trachomatis PCR assay on pooled specimens was compared with individual testing using 370 FCU specimens from asymptomatic men presenting to an STD clinic. Cost savings associated with pooling were estimated. Results: Using mock specimens, the sensitivity and specificity of the Amplicor PCR assay were not aVected by pool sizes of two and five, but at a pool size of 10 decreased sensitivity due to inhibition was observed in one of five mock pools when the pooling method which involved no dilution was used. Archived FCU specimens from a study of 370 asymptomatic men were combined consecutively into 74 pools of five and tested by PCR. Of the 18 pools that contained positive specimens, 17 were PCR positive. Compared with testing FCU specimens individually, pooling resulted in a sensitivity of 95%, specificity of 100%, and a cost savings of 57% based on reduced number of tests required. Conclusion: Depending on the prevalence of infection, pooling of FCU specimens for PCR testing may result in cost savings compared with testing specimens individually. Further evaluations to validate this strategy using fresh FCU specimens are needed. (Sex Transm Inf 1998;74:66-70)

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Evaluation of the Amplicor Chlamydia trachomatis test versus culture in genital samples in various prevalence populations.

Cited by 24 publications

References 15 publications

Improved PCR detection of Chlamydia trachomatis by using an altered method of specimen transport and high-quality endocervical specimens

Improved PCR detection of Chlamydia trachomatis by using an altered method of specimen transport and high-quality endocervical specimens

Developmental factors of urethral human papillomavirus lesions: correlation with circumcision

Pooling of urine specimens for PCR testing: a cost saving strategy for Chlamydia trachomatis control programmes

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