Evaluation of the anti-inflammatory activity of riparin II (O-methil-N-2-hidroxi-benzoyl tyramine) in animal models

Abstract

Riparin II (RipII), an alkamide isolated from the green fruit of Aniba riparia, was tested in the various animal models of inflammation to investigate its anti-inflammatory activity. Male Wistar rats (180-240g) were treated with RipII by gavage at doses 25 or 50mg/kg, before initiating the inflammatory responses. The tests used were paw edema induced by carrageenan, dextran, histamine or serotonin; peritonitis induced by carrageenan and fMLP, as well as the measurement of MPO activity, TNF-α and Il-1β amount in the peritoneal fluid. In the animal models of carrageenan and dextran-induced paw edema, the animals treated with RipII showed lower edema than those of the control group. Treatment with RipII also reduced the paw edema induced by histamine but not serotonin. In the carrageenan-induced peritonitis model, treatment with RipII reduced leukocyte migration, the MPO activity and the amount of TNF-α and IL-1β in the peritoneal fluid. In summary, these results indicate that RipII has an anti-inflammatory activity in chemical models of acute inflammation. RipII might be directly or indirectly inhibiting the activity, production or release of pro-inflammatory mediators involved in the generation of the pain associated with inflammation.

Polysaccharide fraction isolated from P assiflora edulis inhibits the inflammatory response and the oxidative stress in mice

“…Neutrophil migration to an injury site results in the overproduction of ROS, and leads to oxidative stress and damage of important biomolecules, such as proteins or DNA [36]. Evidence has implicated intracellular ROS production modulating the release of mediators of inflammation [37]. ROS can also regulate adhesion molecule expression on the endothelium, directly influencing the increase of cell recruitment to inflammation sites [37]…”

Spectral and computational features of the binding between riparins and human serum albumin

“…Different species of this family Riparin I, for example, demonstrated antinociceptive activity [8] and antianxiety effects in animal models [9]. Studies with riparin II revealed that it may inhibit directly or indirectly the activity, production or release of pro-inflammatory mediators involved in the generation of pain associated with inflammation [7], and produces significant antidepressant activity [10]. Riparin III, administered intraperitoneally or orally, also showed anxiolytic and antidepressant activity in rodents [10][11][12].…”

“…(7)), for the total concentration protein, [L] free and Συ i can be determined by the linear regression of a plot of [L] T against [M] T (see insert inFig. 5)[41] .…”

Riparin B, a Synthetic Compound Analogue of Riparin, Inhibits the Systemic Inflammatory Response and Oxidative Stress in Mice

“…Alkaloids derivatives of plants possess potent anti-oxidative and anti-inflammatory properties [6]. It has been described in the literature that several modified or synthetic compounds of substances extracted from Aniba riparia possess several biological effects, which include an anti-nociceptive effect (Riparin I) [7], an anti-inflammatory effect (Riparin II) [8], and an anxiolytic effect (Riparin III) [9]. Some synthetic Riparin-derived compounds (Riparin A, B, C, D, E, and F) also demonstrate an anti-oxidant action in vitro [5].…”