Evaluation of the anti-inflammatory activity of <i>Tinospora cordifolia</i> (Willd.) Miers chloroform extract – a preclinical study

Philip, Sheena; Tom, Greeshma; Vasumathi, Asha V.

doi:10.1111/jphp.12932

Cited by 34 publications

(35 citation statements)

References 36 publications

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“…Tinospora cordifolia has an effect on the expression of cyclooxygenase-1, tumor necrosis factor-a, iNOS genes, cytokines (IL-6, IL-1b and PGE2) and NF-kB activation and has the potential to reduce the expression of the COX-2 enzyme and thereby reducing the expression of TNF-a, synthesis of IL-1B and IL-6 and other pro-inflammatory interleukins (Tiwari et al, 2018). The phytosterols such as stigmasterol and b-sitosterol found in T. cordifolia are found to be responsible for the inhibition of cyclooxygenase-2 synthesis and adhesion molecules in TNF-a stimulated by cells (Philip et al, 2018). Stigmasterol inhibits prostaglandin E2 and other matrix degradation mediators (Joshi & Kaur, 2016;Sharma et al, 2019;Singh & Chaudhuri, 2017;Tiwari et al, 2018;Upadhyay et al, 2010;Yanez et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Screening of phytochemical compounds of Tinospora cordifolia for their inhibitory activity on SARS-CoV-2: an in silico study

Krupanidhi

Peele

Venkateswarulu

et al. 2020

Journal of Biomolecular Structure and Dynamics

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In the present study, we explored phytochemical constituents of Tinospora cordifolia in terms of its binding affinity targeting the active site pocket of the main protease (3CL pro) of SARS-CoV-2 using molecular docking study and assessed the stability of top docking complex of tinosponone and 3CL pro using molecular dynamics simulations with GROMACS 2020.2 version. Out of 11 curated screened compounds, we found the significant docking score for tinosponone, xanosporic acid, cardiofolioside B, tembetarine and berberine in Tinospora cordifolia. Based on the findings of the docking study, it was confirmed that tinosponone is the potent inhibitor of main protease of SARS-CoV-2 with the best binding affinity of À7.7 kcal/mol. Further, ADME along with toxicity analysis was studied to predict the pharmacokinetics and drug-likeness properties of five top hits compounds. The molecular dynamics simulation analysis confirmed the stability of tinosponone and 3CL pro complex with a random mean square deviation (RMSD) value of 0.1 nm. The computer-aided drug design approach proved that the compound tinosponone from T. cordifolia is a potent inhibitor of 3CL main protease of SARS-CoV-2. Further, the in vitro and in vivo-based testing will be required to confirm its inhibitory effect on SARS-CoV-2.

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Section: Introductionmentioning

confidence: 99%

Screening of phytochemical compounds of Tinospora cordifolia for their inhibitory activity on SARS-CoV-2: an in silico study

Krupanidhi

Peele

Venkateswarulu

et al. 2020

Journal of Biomolecular Structure and Dynamics

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“…The present study was aimed to elucidate the anti-cancer activity of chloroform (Chl-TCE) and hexane extracts (Hex-TCE) of T. cordifolia. Previous studies from our lab have reported the differentiation inducing potential and anti-cancer activity of 50% ethanolic extract of T. cordifolia (TCE) [9][10]. TCE was further fractionated by chloroform, hexane, ethyl acetate and butanol to explore the nature of expected active compounds and to reduce the effective dose on glioblastoma and neuroblastoma cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…TCE was further fractionated by chloroform, hexane, ethyl acetate and butanol to explore the nature of expected active compounds and to reduce the effective dose on glioblastoma and neuroblastoma cell lines. Chloroform and hexane fractions were found to possess anti-proliferative activity at very low doses (10 μg/ml of Chl-TCE and 15 μg/ml of Hex-TCE) as compared to effective doses of TCE (250 μg/ml and 350 μg/ml) for both the cell lines [9][10], whereas, ethylacetate and butanol fraction did not show anti-cancer activity ( Supplementary Fig. 1).…”

Section: Discussionmentioning

confidence: 99%

“…It is also known as "Amrita" due to its adaptogenic and immuno-modulatory properties [6]. T. cordifolia has been traditionally used for treatment of fever, gout, arthritis, dysentery, diarrhea, diabetes, anemia, asthma and psoriasis [8][9]. This plant contains glycosides such as tinocordiside, tinocordifolioside, cordiosides and palmitosides, steroids such as β-sitosterol, ecdysterone, alkaloids such as palmatine, berberine, magnoflorine, 11-hydroxymustakone, some diterpenoid lactones and aliphatic compounds as active constituents responsible for its medicinal properties.…”

Section: Introductionmentioning

confidence: 99%

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Anti-brain cancer activity of chloroform and hexane extracts of Tinospora cordifolia Miers: an in vitro perspective

Sharma

Saggu

Mishra

et al. 2019

Annals of Neurosciences

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Background: Plants have been suggested as safest source of therapeutic agents, with multi targeted mode of action and least side effects. Tinospora cordifolia, commonly known as Guduchi in India, is one of the most highly valued herbs in Ayurvedic medicine. It possesses potential anti-cancer, anti-inflammatory, hepatoprotective, anti-diabetic, immune-stimulatory and various other beneficial activities. Purpose: The present study was aimed to investigate the differentiation inducing potential of chloroform and hexane extracts of T. cordifolia using U87MG glioblastoma and IMR-32 neuroblastoma cell lines as model system. Results: Chloroform (Chl-TCE) and hexane (Hex-TCE) extracts significantly reduced the rate of proliferation and induced cell differentiation as evidenced by MTT assay and immunostaining for GFAP and MAP-2 in glioblastoma and neuroblastoma, respectively. Further these extracts increased the expression of stress markers HSP70 and Mortalin and induced senescence. Chloroform and hexane extracts also inhibited the migration of U87MG glioblastoma and IMR-32 neuroblastoma as indicated by wound scratch assay and supported by reduced expression of NCAM. Furthermore these extracts are not toxic to normal cells as they showed no inhibitory effects on primary astrocytic and neuronal cultures. Conclusions: The present study suggests that chloroform and hexane extracts of T. cordifolia retard the rate of proliferation, induce differentiation and inhibit migration of human glioblastomas and neuroblastomas, thus may act as potential phytotherapeutic intervention in treatment of neural cancers.

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“…Phytochemical analysis of Tinospora cordifolia (TC) revealed that it contains alkaloids, glycosides, sesquiterpenoids, lactones, and steroids 7 . So far studies have been carried out to evaluate the anti-inflammatory and immuno-modulatory properties of TC 8,9 . The effect of TC against AI has not been studied yet and therefore we evaluated the effectiveness of TC for the treatment of AI.…”

Section: Introductionmentioning

confidence: 99%

Tinospora cordifolia protects against inflammation associated anemia by modulating inflammatory cytokines and hepcidin expression in male Wistar rats

Ghatpande

Misar

Waghole

et al. 2019

Sci Rep

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Systemic iron homeostasis dysregulation is primarily associated with inflammation- associated anemia (AI) due to hepcidin up-regulation. Tinospora cordifolia (TC) has shown remarkable anti-inflammatory properties and has been found useful in the treatment of inflammatory disorders. However, the effects and mechanisms of TC on AI have not been studied yet. We conducted in vivo and in vitro studies to evaluate the effect of TC on AI. HPLC studies were also carried out to find out active constituents in TC extract. Model system exhibiting AI was developed by repeated injections of HKBA in Wistar rats. TC treated groups showed significantly higher levels of Hb and RBC count compared to the inflammatory control group. TC treatment showed reduction in the expression of the HAMP (hepcidin) gene in the rat liver. TC extract also inhibited gene expression of inflammatory cytokines (TNF-α, IL-1β) and decreased NO production in RAW 264.7 cells. The HPLC analysis revealed the presence of tinosporaside, which could have synergistically contributed to the above findings. Overall results indicate that TC therapy was able to maintain circulating iron through reduction of inflammatory cytokines and expression of hepcidin in rats.

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Evaluation of the anti-inflammatory activity of Tinospora cordifolia (Willd.) Miers chloroform extract – a preclinical study

Abstract: These findings encourage us strongly to focus on CETC to develop anti-inflammatory drugs with lower degree of inhibition to the constitutively expressing COX-1.

Cited by 34 publications

References 36 publications

Screening of phytochemical compounds of Tinospora cordifolia for their inhibitory activity on SARS-CoV-2: an in silico study

Screening of phytochemical compounds of Tinospora cordifolia for their inhibitory activity on SARS-CoV-2: an in silico study

Anti-brain cancer activity of chloroform and hexane extracts of Tinospora cordifolia Miers: an in vitro perspective

Tinospora cordifolia protects against inflammation associated anemia by modulating inflammatory cytokines and hepcidin expression in male Wistar rats

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