Evaluation of the Anti-Inflammatory Capacity of Beta-Sitosterol in Rodent Assays

Paniagua-Pérez, Rogelio; Flores-Mondragón, Gabriela; Reyes-Legorreta, Celia; Herrera-López, Brígida; Cervantes-Hernández, Isabel; Madrigal-Santillán, Osiris; Morales-González, José A.; Álvarez-González, Isela; Madrigal-Bujaidar, Eduardo

doi:10.21010/ajtcam.v14i1.13

Cited by 99 publications

(59 citation statements)

References 44 publications

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“…Similar findings were observed on U937 AML cells, involving caspase 3 activation, and an increase in the Bax/Bcl2 ratio [58]. Other studies suggest the anti-inflammatory capacity of β-sitosterol [59], as well as its antihyperglycemic and insulin-releasing activities [60]. Other compounds in AELE remain unidentified and require further investigation.…”

Section: Discussionsupporting

confidence: 75%

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

Ammoury

Younes

Khoury

et al. 2019

BMC Complement Altern Med

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BackgroundThe edible fruit Annona cherimola has previously shown many nutritional and medicinal properties. The current study evaluates the anti-cancer and anti-proliferative properties of Annona cherimola ethanolic leaf extract (AELE) on Acute Myeloid Leukemia (AML) cell lines cultured in vitro (Monomac-1 and KG-1).MethodsThe anti-proliferative effect of A. cherimola ethanolic leaf extract was evaluated via cell viability assay. Its pro-apoptotic effect was assessed through Cell Death ELISA and dual Annexin V/PI staining. To further investigate the molecular mechanism by which the extract promoted apoptosis and inhibited the proliferation of the AML cells used, apoptotic protein expression was determined through western blots. Extract composition was elucidated by Gas Chromatography-Mass Spectrometry (GC-MS).ResultsOur results showed that the treatment with A. cherimola ethanolic leaf extract exhibited an inhibitory effect on the proliferation of both cancer cell lines used in a dose- and time-dependent manner, with no toxic effects on normal mononuclear cells (MNCs) isolated from human bone marrow. This effect was mediated by DNA fragmentation and apoptosis, as revealed by Cell Death ELISA and dual Annexin V/PI staining. Western blot analysis revealed a Bax/Bcl2 dependent mechanism of apoptosis, as well as PARP cleavage, confirming the apoptotic results observed previously. These effects may be attributed to the presence of terpenes which constitute a large component of the leafy extract, as revealed via GC-MS.ConclusionAll the data presented in our study show that the terpene-rich A. cherimola ethanolic leaf extract exhibits an anti-proliferative and pro-apoptotic effect on the AML cell lines used.

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Section: Discussionsupporting

confidence: 75%

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

Ammoury

Younes

Khoury

et al. 2019

BMC Complement Altern Med

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“…Phytosterols have been well-documented for having anti-inflammatory, antioxidant and anticancer effects [37–39]. Previous studies have reported the potential anti-inflammatory activity of β-sitosterol using in vitro and in vivo models [40, 41]. Enriched content of β-sitosterol in VISPO could possibly contribute to the anti-inflammatory effect in BPH model.…”

Section: Discussionmentioning

confidence: 99%

A phytosterol-enriched saw palmetto supercritical CO2 extract ameliorates testosterone-induced benign prostatic hyperplasia by regulating the inflammatory and apoptotic proteins in a rat model

Sudeep¹,

Venkatakrishna²,

Amrutharaj³

et al. 2019

BMC Complement Altern Med

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Background Benign prostatic hyperplasia (BPH) is a pathological condition affecting older men. BPH complications often lead to deterioration in the quality of life. Serenoa repens (Saw Palmetto) is used for treating lower urinary tract infections in traditional medicine. Methods This study was performed to compare the efficacy of β-sitosterol enriched saw palmetto oil (VISPO) and conventional saw palmetto oil (SPO) extracted using supercritical fluid extraction, in alleviating the BPH complications using testosterone-induced BPH model rats. The animals received testosterone (5 mg/kg s.c.) with or without SPO and VISPO (200 and 400 mg/kg b.w.) or Finasteride (1 mg/kg b.w.) p.o. for 28 days. At the end of the experiment, overnight fasted animals were euthanized, blood samples collected for serum analysis of testosterone. Prostate tissue histomorphology was examined by hematoxylin and eosin (H&E) staining. Western blot analysis was performed using prostate tissue homogenates. Results VISPO exhibited superior efficacy compared to SPO as evident from the significant decrease in prostate weight to body weight ratio, serum testosterone level and increase in growth inhibition of prostate tissue compared to BPH group (p < 0.001). Histological examination of prostate tissue samples showed that VISPO treatment was comparatively better than SPO in improving the hyperplastic patterns. Further, VISPO significantly regulated the expression of inflammatory and apoptotic marker proteins in BPH rats. Conclusion Our data provide experimental evidence that β-sitosterol enriched saw palmetto oil could be higher efficacious in treating the BPH complications compared to the conventional saw palmetto oil preparations.

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“…Dosing Protocol: Control groups were given distilled water only. Aspirin 300 mg per 70 kg and Ibuprofen 200 mg per kg were given as standard drug in hot plate & tail flick and acetic acid induced paw-edema model respectively 10,11 . Single and double dose groups were given aqua distillate of Rosa damascena Mill.…”

Section: Materials and Methods: Selection Of Animalsmentioning

confidence: 99%

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2018

IJPSR

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Rosa damascena Mill. also known as Damask rose, Persian rose or Gul-e-Muhammadi of Rosaceae family is an aromatic small plant cultivated all over the world for its visual beauty, fragrances and therapeutic potential. The plant is rich in tannins, flavonoids, glycosides, carboxylic acids and ascorbic acid. In addition to its aromatic properties, the plant possesses laxative, anti-oxidant, anti-bacterial, wound healing, skin health, cardiotonic, hypnotic and cough suppressant activities. The medicinal uses reported from this plant are of its methanolic, ethanolic or chloroformic extract and of its essential oil. This research is planned to investigate the analgesic and anti-inflammatory potential of aqua distillate of Rosa damascena Mill. flower in two different doses i.e. 250 mg/kg and 500 mg/kg using Hot Plate, Tail Flick and acetic acid induced paw-edema method. Also as recent studies reported that alcoholic extract of Rosa damascena possesses analgesic activity and aqueous extract does not, so this study also aimed to evaluate these activities after its distillation in aqueous medium.

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Evaluation of the Anti-Inflammatory Capacity of Beta-Sitosterol in Rodent Assays

Cited by 99 publications

References 44 publications

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

A phytosterol-enriched saw palmetto supercritical CO2 extract ameliorates testosterone-induced benign prostatic hyperplasia by regulating the inflammatory and apoptotic proteins in a rat model

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