Evaluation of the anti-Trichophyton activity of a prodigiosin analogue produced by γ-proteobacterium, using stratum corneum epidermis of the Yucatan micropig

Nakashima, Takuji; Yamaguchi, Kensei; Oda, Tatsuya; Kato, Yoko

doi:10.1007/s10156-005-0376-0

Cited by 14 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Preparation of Red Pigment (PG-L-1) and Prodigiosin Produced by Serratia marcescens JCM 1239 PG-L-1 was purified from strain MS-02-063 as described by Nakashima et al 1) The pigment was dissolved in methanol to give a stock solution of 10 mg/ml, and was stored in dark at Ϫ80°C. Serratia marcescens JCM 1239 that produces prodigiosin (PG ser ) was obtained from Riken Cell Bank (Tsukuba, Ibaragi, Japan).…”

Section: Methodsmentioning

confidence: 99%

Apoptosis-Mediated Cytotoxicity of Prodigiosin-Like Red Pigment Produced by .GAMMA.-Proteobacterium and Its Multiple Bioactivities

Nakashima

Tamura

Kurachi

et al. 2005

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

A bacterial strain (MS-02-063), which produces red pigment, was discovered from our library established with more than 20000 microorganisms isolated from coastal area of Nagasaki prefecture, Japan. Comparison of 16S rDNA gene sequence of strain MS-02-063 with sequences from GenBank demonstrated that this strain belongs to the g-proteobacterium species. Recent our study has demonstrated that the highly purified red pigment showed potent antimicrobial activity against pathogenic microorganisms such as Staphylococcus aureus and dermatophytes. 1)Prodigiosin (PG) analogues belonging to a family of natural red pigments are characterized by a common pyrrolydipyrrolylmethene skeleton structure with a C-4 methoxy group, and several PG-like pigments have been isolated from certain microorganisms 2-4) including some genera of actinomycetes, Pseudoalteromonas denitrificans, and Serratia marcescens. Representative members of PG analogues are prodigiosin, undecylprodigiosin, cycloprodigiosin hydrochloride, metacycloprodigiosin, and nonylprodigiosin. 5) Some of PG analogues have been reported to have several biological activities such as immunosuppressive, antimalarial, antimicrobial and cytotoxic activities. [6][7][8][9][10][11] Although the exact cytotoxic mechanisms of PG family members are not fully clarified yet, apoptotic mechanism has been proposed for some PG analogues. For instance, it has been reported that cycloprodigiosin hydrochloride induces apoptosis in cancer cells both in vitro and in vivo, with high efficiency in liver cancer, breast cancer cell lines, and colon cancer cells, [12][13][14] with no apparent toxicity to normal cells. 14) PG produced by S. marcescens also induces apoptosis in haematopoietic and gastrointestinal cancer cell lines, but no marked toxicity to non-malignant cell lines. 7,15,16) Considering the high selectivity of PG family members to cancer cells, these pigments may be promising candidates for new anticancer drugs with unique action mechanism. Regarding the structure-activity relationship, it has been reported that a nitrogen-containing heterocyclic A-pyrrole ring with the lone-pair nitrogen electrons in conjugation with the tricyclic frame and a C-4 alkoxy are important for the biological activities of PG analogues. 17)Although there are extensive studies on the cytotoxicities of PG analogues produced by S. marcescens and P. denitrificans, 4,16) only limited information on the bioactivities of PG analogues produced by g-proteobacterium is available. In this study, we examined the cytotoxic effects of PG analogue (PG-L-1), produced by g-proteobacterium (strain MS-02-063) on various cultured cell lines. Since U937 cells showed the highest susceptibility to PG-L-1 cytotoxicity, we conducted detailed analysis of cytotoxic mechanism of PG-L-1 in U937 cells especially in terms of apoptotic cell death. To search for new aspect of biochemical activity of PG-L-1, we also examined the effect of PG-L-1 on the O 2 Ϫ generation by 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated U937 cells. ...

show abstract

Section: Methodsmentioning

confidence: 99%

Apoptosis-Mediated Cytotoxicity of Prodigiosin-Like Red Pigment Produced by .GAMMA.-Proteobacterium and Its Multiple Bioactivities

Nakashima

Tamura

Kurachi

et al. 2005

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

show abstract

“…Strain MS-02-063 was phylogenetically closely related to gammaproteobacterium MBIC 3957; however, there were differences in the physiological and biochemical properties (Nakashima et al 2005b). The red pigment produced by strain MS-02-063 had potent antifungal, antibacterial, and in vitro antitumor activities (Nakashima et al 2005a(Nakashima et al , 2005b(Nakashima et al , 2005c. Our chemical structural and biochemical analysis revealed that this red pigment belongs to the prodigiosin family, and we named it PG-L-1.…”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 97%

Mode of action of an antialgal agent produced by a marine gammaproteobacterium against Chattonella marina

Nakashima¹,

Kim²,

Miyazaki³

et al. 2006

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

“…These advantages made the Yucatan minipig a desirable alternative to other species of animals for use in skin flap research 39 . In addition, the skin of minipig has been preferably used for the evaluation of dermal irritation by topical drugs 40,41 , the effect of collagenase in promoting injury and wound healing in vivo 42 , and estimating the antifungal activity of drugs in vitro 43 . On the other hands, there is a report that the domestic pig is a better animal model than the Yucatan minipig for preclinical studies.…”

Section: Dermatotoxicologymentioning

confidence: 99%

Use of Miniature Pig for Biomedical Research, with Reference to Toxicologic Studies

et al. 2007

View full text Add to dashboard Cite

Abstract:The pigs have been a well-recognized experimental animal in biomedical research for many years. Minipigs particularly have gained in massive importance in biomedical research over the last few years. Pigs are increasingly being used as an alternative non-rodent animal species to the dog or monkey in toxicology because of the morphological and physiological similarities between porcine and human organs, especially the skin, cardiovascular system, gastrointestinal tract, and the urinary system. Accumulating data indicate that the minipig can be used for all routes of administration and is preferable to the dog or monkey in many cases. The advantages of the minipig compared to the domestic pig are its smaller size, even at full maturity, slower growth during studies, ease of handling, and controlled genotype as well as microbiologically obvious characteristics. Minipigs also have an advantage over traditional nonrodent animals because of increasing ethical concerns about the use of them in experiments. Reservoir of information from studies using minipigs is the keystone for the future diffusion of them as a good alternative to the non-rodent animals traditionally used in toxicology. (J Toxicol Pathol 2007; 20: 125-132)

show abstract

Evaluation of the anti-Trichophyton activity of a prodigiosin analogue produced by γ-proteobacterium, using stratum corneum epidermis of the Yucatan micropig

Cited by 14 publications

References 26 publications

Apoptosis-Mediated Cytotoxicity of Prodigiosin-Like Red Pigment Produced by .GAMMA.-Proteobacterium and Its Multiple Bioactivities

Apoptosis-Mediated Cytotoxicity of Prodigiosin-Like Red Pigment Produced by .GAMMA.-Proteobacterium and Its Multiple Bioactivities

Mode of action of an antialgal agent produced by a marine gammaproteobacterium against Chattonella marina

Use of Miniature Pig for Biomedical Research, with Reference to Toxicologic Studies

Contact Info

Product

Resources

About