Evaluation of the antibacterial activity of poly-(d,l-lactide-co-glycolide) nanoparticles containing violacein

Martins, Dorival; Costa, Fábio T. M.; Brocchi, Marcelo; Durán, Nélson

doi:10.1007/s11051-010-0037-9

Cited by 34 publications

(26 citation statements)

References 29 publications

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“…The increased biological activity of violacein loaded in nanoparticles has been demonstrated in previous works either with tumoral cells 26 or Staphylococci strains 27 and the addition of derivatives of ascorbic acid on external surface of nanoparticles had been shown to increase the efficiency of dehydrocrotonin loaded in PLGA nanoparticles with a protein mediated uptake mechanism and a shunt of cell death mechanism to receptor-mediated pathways. 29 However, an unexpected observation was reached, since free ascorbic acid exhibits a buffering effect in violacein-mediated toxicity, while the addition of ascorbic acid on the external surface of PLGA nanoparticles loading violacein actually promotes an improvement of the toxicity of this compound.…”

Section: Discussionmentioning

confidence: 64%

“…27 The particles were dispersed in absolute ethanol, incubated for one hour at room temperature to extract the violacein and centrifuged at 10,000 rpm for 30 min to separate the drug from the reminiscent matrix. Polymer recovery was calculated by the expression: (total mass -total mass of recovered violacein)/(amount of polymer used in the preparation).…”

Section: Drug Loading Efficiency and Polymer Recoverymentioning

confidence: 99%

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Antitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein

Martins

Frungillo²,

Anazzetti³

et al. 2010

IJN

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It has been demonstrated that tumoral cells have a higher uptake of ascorbic acid compared to normal cells. This differential characteristic can be used as a way to improve the specificity of antitumoral compounds if combined with polymeric drug delivery systems. The aim of this study was to prepare, characterize and evaluate the antitumoral activity of poly-D,L-(lactide-co-glycolide) 50:50 loading the antitumoral compound violacein and capped with L-ascorbic acid. Nanoparticles were prepared using the nanoprecipitation method and morphologically characterized by scanning electron microscopy (SEM). The average diameter and Zeta potential were determined by photon correlation spectroscopy method (PCS), and assays were carried out to determine the content of ascorbic acid and in vitro drug release kinetics. The antitumoral activity of this system was also evaluated against HL-60 cells by tetrazolium reduction assay. Nanoparticles with size distribution between 300-400 nm and strong negative outer surface (-40 mV) were obtained by this method. Analysis of ascorbic acid content showed that this compound was mainly localized on the external surface of nanoparticles. Violacein loading efficiency was determined as 32% ± 1% and this drug was gradually released from nanoparticles at different rates depending on the composition of the release media. In addition, this system was observed to be 2 × more efficient as an antitumoral compared with free violacein.

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Section: Discussionmentioning

confidence: 64%

Section: Drug Loading Efficiency and Polymer Recoverymentioning

confidence: 99%

Antitumoral activity of L-ascorbic acid-poly-D,L-(lactide-co-glycolide) nanoparticles containing violacein

Martins

Frungillo²,

Anazzetti³

et al. 2010

IJN

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“…Both free and nanoparticlesloaded violacein exhibited antibacterial activity in vitro against S. aureus ATCC 25923 and no significant activity against Escherichia coli and Salmonella enterica. The nanoparticles-loaded violacein were at least three times more effective than free violacein against the sensitive strains (Martins et al, 2010a). This is probably due to the nanoparticles loading an active are internalized in the cell in a more efficient way than the bulk active (or free active), and also a sustained release occurred in the cell leading a high violacein stabilization, since violacein is protected from degradation by cell components.…”

Section: Encapsulated Nanoparticlesmentioning

confidence: 95%

Potential applications of violacein: a microbial pigment

et al. 2011

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Violacein is a versatile pigment from a bacterium Chromobacterium violaceum that exhibits several biological activities and, at present, has gained increasing importance in industrial markets, such as in medicine, cosmetics, and textiles. In this mini-review, we aimed to describe violacein production and to explore its various biological properties in a pharmacological context, including its antioxidant, immunomodulatory, antitumoral, and antiparasitic activities. In addition, its use in the fields of cosmetics, textiles, food, toys, and insecticides has emerged as unusual potential areas of application to be discussed here.

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“…Several investigations have shown that nanoparticles could not be very effective on all different types of bacteria and that the antibacterial effect depends on bacterial type (84). For example, recently Martins et al evaluated the antibacterial activity of PLGA nanoparticles containing violacein against different bacteria (84).…”

Section: Antibiotic Loaded Plga Nanoparticlesmentioning

confidence: 99%

“…For example, recently Martins et al evaluated the antibacterial activity of PLGA nanoparticles containing violacein against different bacteria (84). Although, they showed that the MIC with nanoparticles is 2-5 times lower than free violacein against Staphylococcus aureus, the results failed to show any significant activity against Escherichia coli and Salmonella enterica.…”

Section: Antibiotic Loaded Plga Nanoparticlesmentioning

confidence: 99%