Evaluation of the Antibacterial and Antioxidant Activity of Mimosa rubicaulis and Reinwardtia indica

Abstract

Many plants have the property of wound healing, but most of the people are using costly allopathic medicine for the wound. This might be due to lack of awareness about the traditional uses and lack of scientific study of ethnomedicinal plants. So, this study aimed to carry out the antibacterial and antioxidant activity of two medicinal plants which are used traditionally for wound healing activity, i.e.,Mimosa rubicaulis and Reinwardtia indica. Different parts of Mimosa rubicaulis (root, stem, and leaves) and Reinwardtia indica (flower and leaves) were used for the study. Extractions were done by maceration using ethanol as solvent. Antibacterial activity was carried out by the well diffusion method, and antioxidant activities were screened by DPPH radical scavenging and NO scavenging assays. Extract of M. rubicaulis has shown a weak zone of inhibition towards S. aureus and P. vulgaris whereas R. indica has shown no zone of inhibition towards selected bacterial strains. Leaf, root, and stem extracts of M. rubicaulis have shown potent antioxidant activity, i.e., IC50 value of 9.8 µg/ml, 10.19 µg/ml, and, 13.64 µg/ml, respectively. Similarly, leaf extract of R. indica exhibited antioxidant activity with an IC50 value of 19.73 μg/ml. The percentage inhibition of NO radicals of root and stem of M. rubicaulis and leaves of R. indica was 31%, 21%, and 22%, respectively. Out of these two plant species, M. rubicaulis had shown antibacterial activity towards selected microorganism, but antioxidant activity was shown by both plant species. These properties on above mentioned two plant species might help for the development of a marketed formulation for antibacterial and wound-healing agent since wound healing is promoted by antibacterial and antioxidant activities.

Formulation and Evaluation of Quality Parameters of Effervescent Granules from the Potent Antioxidant between Two Variants of the Adaptogenic Herb Ocimum tenuiflorum L.

“…The antioxidant activities of the prepared plant extracts were investigated by the DPPH radical scavenging assay, a very simple and reliable method to evaluate the antioxidant activity of plant extracts. DPPH forms a stable molecule when it accepts an electron or a hydrogen atom and thus can be used in the determination of radical scavenging activity of natural products [ 36 , 37 ]. Free radicals, which are present in our biological systems, may oxidize all the biological molecules such as proteins, nucleic acid, and lipids and initiate degenerative diseases [ 38 , 39 ].…”

“…Free radicals, which are present in our biological systems, may oxidize all the biological molecules such as proteins, nucleic acid, and lipids and initiate degenerative diseases [ 38 , 39 ]. Antioxidants can scavenge the free radicals generated within the body and also play a great role in inhibiting the chain reaction of lipid peroxidation [ 20 , 36 ]. In the present study, the antioxidative properties of both standard drug and plant extracts increased with increasing concentration.…”

Anti-bacterial, Anti-oxidant and other Phytochemical Properties of Datura innoxia leaves

“…The antioxidant activity of the sample is considered higher with the lower IC50 values. According to one report (50), the extract that possesses an IC50 value from 10 to 50 µg/ml exhibit the highest antioxidant activity, 50-100 µg/ml exhibit minimum antioxidant activity and >100 µg/ml is considered to possess weak antioxidant activity (51,52).…”

A Comparative Study on Ascorbic Acid Concentration, Total Phenol, and Flavonoid Content in Citrus Species Grown in a Different Region of Western Nepal

“…Plants are a good source of phytochemicals such as phenol, flavonoid, alkaloids, sterols, terpenoids, phenolic acids, stilbenes, lignans, tannins, and saponins, and some plant also contains vitamin C (ascorbic acid), vitamin E, and carotene [ 1 ]. Due to the presence of these phytochemicals, plants show many pharmacological activities such as antioxidant activity, wound healing properties, anti-inflammatory activity, pain healing, antidiarrheal activity, antimicrobial, and anticancer activity [ 2 , 3 ].…”