Evaluation of the Antibacterial Impact of the Conjugated Gentamycin-biosynthesized Silver Nanoparticles on Resistant Pseudomonas aeruginosa Isolates In Vitro

Abstract

The expanding resistance of pathogenic bacteria to antibiotics casts a serious threat to the public health. Thus, a new strategy is required to resolve this problem. This study aims to compare the antibacterial impact of biosynthesized silver nanoparticles (Bio-AgNPs), gentamycin (GEN), and the conjugation of biosynthesized silver nanoparticles and gentamycin (GEN:Bio-AgNPs) on multidrugresistant Pseudomonas aeruginosa isolates. The characteristic properties of Bio-AgNPs were detected by the following analyses: ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), Zeta potential, transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Depending on micro-dilution assay, the minimum inhibitory concentration (MIC) of the tested subjects (Bio-AgNPs, GEN, GEN:Bio-AgNPs) are calculated to be 112 ± 400, 1 536 ± 525, and 49 ± 18.8 μg/mL, respectively. The obtained results confirmed that the GEN:Bio-AgNPs have greater potency effect than Bio-AgNPs and GEN alone, showing that AgNPs of low concentration can potentially enhance the effect of GEN against resistant P. aeruginosa.