Evaluation of the antibacterial influence of silver nanoparticles against fish pathogenic bacterial isolates and their toxicity against common carp fish

Abstract

At present, nanotechnology and nanomaterials are being emerged very quickly and gained the attention of researchers due to their frequent application history, especially their antibacterial effects against certain bacterial isolates. Therefore, the present study was aimed to check the antibacterial influence and toxic effects of the market available (Chemical Fabricated) silver nanoparticles (AgNPs). For this purpose, the AgNPs were obtained from the local market and used against fish pathogenic bacterial species. The highest zone of inhibition was observed against Aeromonas hydrophila (27.53 ± 0.69). Moreover, the AgNPs were exposed to the common carp fish for toxicity and toxic effects. The highest mortality was seen at the highest concentration (0.09 mg/L) of AgNPs. Finally, the AgNPs mainly were accumulated in the liver, followed by the intestine, gills, and muscles. The intestine absorbed the nanomaterials; therefore, it is critical to check the influence of these AgNPs on the fish intestinal bacterial community via MiSeq Illumina Sequencing.

Bio‐reductive synthesis of silver nanoparticles, its antibacterial efficiency, and possible toxicity in common carp fish (Cyprinus carpio)

“…Recently, a study reported the antibacterial activity of plant ( C. crispus ) mediated AgNPs in which the highest zone of inhibition was formed against gram‐positive bacteria Micrococcus luteus (7 ± 0.4 to 7.7 ± 0.5 mm). The conducted study was meaningful however, the inhibitory zones formed in our study were far larger than these previous conducted studies (Urnukhsaikhan et al, 2021; Kakakhel et al, 2021b).…”

Effects of polysaccharide-based silver and selenium nanoparticles on growth performance, biochemical parameters, and immune response of Cyprinus carpio

“…AgNPs showed higher RPS because it inhibits bacterial cells through the binding of Ag + ions to proteins, enzymes, DNA, RNA, and other micro and macromolecules leading to disruption of microbial metabolism and survival [96] . AgNPs are known to exhibit antimicrobial activity against several aquatic pathogens such as A. salmonicida, A. hydrophila , and A. veronii by decreasing total bacterial count and enhancing survivability in challenged fish [ 97 , 10 , 98 ]. Also, it was reported that the SeNPs used as an immunostimulant in fish could improve the innate immune response via regulating the redox-sensitive transcription factors and activating stress-related genes thus it prevents aquatic infection [99] .…”

“…Among all the nanoparticles, silver nanoparticles (AgNPs) and selenium nanoparticles (SeNPs) have gained much attention in the aquaculture field to control various forms of bacterial, fungal, and viral diseases especially to combat antibiotic-resistant pathogens [ 8 , 9 ]. It has been previously reported that the AgNPs showed bactericidal activity against Aeromonas species, and Vibrio harveyi in various aquatic animals [10] . Previously, it was reported that fish's innate immune system and antioxidant enzyme activity could enhance the defensive mechanism against A. hydrophila infection [11] .…”

Interactive analysis of biosurfactants in fruit-waste fermentation samples using BioSurfDB and MEGAN

“…Several studies have used microorganisms isolated from fruits in the production of various molecules of biotechnological interest, such as mannitol, hydrogen, organic acids, biofuels and biosurfactants 14 – 18 . In addition, fruit waste and residues generated by fruit-processing industries can also be used as a renewable and low-cost carbon source for fermentative processes 12 , 19 – 21 . To allow better exploitation of such waste as a source of useful microorganisms, a clearer understanding of the taxonomic and functional diversity of the present microbes is required.…”