Evaluation of the Antifungal Effect Vitis vinifera Extract on Candida albicans

Abstract

Objective: This research analyzed in vitro the antifungal activity of the solution composed by grapes seed extract Vitis vinifera (Doctor's A-Z®) against Candida albicans (ATCC 20231) species aiming to determine the minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC). Methods: To that, were used micro dilution boards of 96 wells where the strain of Candida. Was tested the diluted solutions in distilled water on the concentrations 0.20 g/mL, 0.30 g/mL and 0.40 g/mL. As growing control were used growing fungous, to test the viability of the strains. As positive control, was used nystatin (commercial suspension 100,000 UI/mL) and as sterility control of growing way. The tests were conducted in triplicate and the data obtained were analyzed through the exam Kruskal-Wallis, with significance level of 95% (p<0.05). Results: The results indicated that all the samples were sensible to the analyzed extract and the CIM was obtained with the solution of 0.30 g/mL (30%). However, wasn't observed a fungicide effect (MFC) on the tested concentrations. Conclusion: We conclude that the grapes seed extract can be an alternative against the gender Candida, however are necessary larger studies to prove their ant fungous action.

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“…Vitis vinifera seed hydroalcoholic extract has been shown to inhibit C. albicans at concentrations between 5.7 and 20.2 mg/L minimal inhibitory concentration (MIC), depending on the cultivars, the effect being due to the its high content of polymeric flavan-3-ols [55]. In another study, inhibition of C. albicans was observed at a concentration of 0.3 g/mL MIC of Vitis vinifera seed aqueous extract [54]. The significant difference in MIC between the two studies could be determined by the type of cultivar, agronomic conditions, and/or the method of extraction.…”

“…The antimicrobial activity of thyme essential oil (TEO) against several pathogenic species is well known, and several formulations that include TEO for the treatment of vaginal infections have been developed [49][50][51][52][53]. Several other studies have shown the antifungal effect of Vitis vinifera seed extract [54,55]. The lactic acid produced by the Lactobacillus genus from vaginal microbiota maintains a slightly acidic pH in the cytosol, at which pathogenic species cannot proliferate.…”