Evaluation of the antimicrobic effects of disinfectants recommended for use in orthopaedic dentistry for the treatment of dental impressions

Abstract

The assessment of the effect of disinfectants on microbiota and the rationale for the choice of drugs for processing dentures and prostheses themselves remains open and needs to be carefully studied. For example, the effect of chemical preparations of the group of quaternary ammonium derivatives (HR) on mixed microbial biofilms formed by representatives of the oral microbiota has not been studied sufficiently, despite their wide practical use. The purpose is to carry out a comparative assessment of in vitro activity in relation to sanitary significant strains of microorganisms of a number of alkaline combined disinfectants – derivatives of HR and polyhydric alcohols, depending on the degree of dilution. Material and methods. A conventional bacteriological test of dental swabs and an in vitro experiment were performed dilutions of domestic disinfectants: Megadez Orto (VladMiva, RF), Windelin (Boson, RF) and Trilox (Biotorg, RF) were prepared in sterile cardiovascular broth from 1:200 to 1: 200,000 and microbial population growth curves were assessed using a bioreactor RS. The study used 3 strains of sanitary significant bacteria: Staphylococcus aureus, Escherichia coli, Bacillus cereus. Results and discussion. The results of the in vitro experiment indicate that the working solutions of the tested disinfectants in dilutions of 1:200 and 1:20 have bactericidal activity against all test strains of S. aureus, E. coli, B. cereus taken. For the eradication of spore-forming bacilli, slightly higher concentrations of disinfectants are required than for undisputed Gram-positive and Gram-negative bacteria. Conclusions. According to the level of decrease in the activity of ET, these drugs can be arranged as follows in descending degree: Trilox > Wendelin > Megadez-Orto. The clear advantage of the Trilox disinfectant is the statistically justified more pronounced activity against spore-forming B. cereus bacilli.