EVALUATION OF THE ANTIOXIDANT AND Α-AMYLASE INHIBITORY ACTIVITIES OF Mitragyna inermis (WILLD) O. KUNTZE AND Tamarindus indica LINN.

Abstract

The current study was conducted to explore the antioxidant and anti-diabetic potential of Tamarindus (T.) indica and Mitragyna(M.) inermis aqueous leaves extracts. Along with this, the estimation of available total phenolics and total flavonoids contents was also carried out to establish the scientific background of the antioxidant and anti-diabetic potential of used plants. For estimation of total phenolics and total flavonoids contents, Folin-ciocalteu and Aluminium chloride (AlCl3) colorimetric method was used respectively. Antioxidant activity of prepared aqueous extracts was determined by various in vitro assays such as DPPH•, ABTS•+, FRAP assay while for the determining anti-diabetic potential of two used aqueous extracts plants, α-amylase inhibition assay was performed using the 3,5- dinitrosalicylic acid method. The results of the current study suggested that the aqueous extracts of both species have similar polyphenol content while in the case of flavonoid content, it was reported higher in T. indica leaves (9.90 mg QE/100 mg extract) and this is significantly different (p˂0.001) from the aqueous leaves extract of M. inermis. The results revealed that both plant extracts effectively scavenged DPPH and ABTS radical which suggested the strong antioxidant potential of used extracts. Besides, plant extracts also exhibited higher Fe (III) reducing activities. When these results were compared with standard treatment, highly significant difference was reported at 10 mg/ml extract doses (p˂0.001). Both leaf extracts exhibit remarkable α-amylases inhibitory activity, this was reported 89.55% with T. indica while 75.65% with M. inermis at 10 mg/mL extract. Hence leaves extracts of selected plants have significant antioxidant and anti-diabetic potential which justify the use of these two plants in traditional remedies. Further works are necessary for the isolation, characterization, and identification of active antioxidant and anti-diabetic molecules from the aqueous extracts of T. indica and M. inermis, which may serve as a potential natural source of antioxidant and anti-diabetes.

Antimalarial, cytotoxic and antioxidant activities of 14 medicinal plants from Sudan

“…The observed high antioxidant activity of some plant extracts may be attributed to high concentrations of phenolic compounds with potential protective effects (Spanou et al, 2010). Furthermore, these findings supported previous results on the antioxidant activity of S. hermonthica (Kiendrebeogo et al, 2005), C. grandis (Deshpande et al, 2011), C. quadrangularis (Prabhavathi et al, 2016;Tiwari et al, 2021), D. hirtiflora (Adeniran et al, 2020;Sonibare & Abegunde, 2012), G. alata (Zheleva-Dimitrova et al, 2017 and M. inermis (Ouédraogo et al, 2020) using different extraction solvents.…”

Botanical aspects, phytochemicals, and toxicity of Tamarindus indica leaf and a systematic review of antioxidant capacities of T. indica leaf extracts

“…The results of the study by Razali et al ( 29 ) showed that the methanolic extract obtained from maceration expressed antioxidant capacity close to that of the standard compound rutin (1.65 vs. 1.72 mmol TE/g dried weight), as ethyl acetate and hexane extracts possessed lower capacities (0.7 and 0.5 mmol TE/g dried weight, respectively). The ABTS •+ radical scavenging capacity determined in Ouédraogo et al's ( 47 ) study using aqueous extract was 7067.6 μmol AAE/g extract, which can be calculated as half of the positive control, quercetin (14550.2 μmol AAE/g).…”

“…Eight studies conducted by Krishnaveni et al resulted in 2.3–8.3 mg AAE/g extract ( 30 , 31 , 35 – 37 , 39 – 41 ). Ouédraogo et al ( 47 ) reported a value of 667.26 μmol AAE/g extract, which was ~10% of standard quercetin (6034.6 μmol AAE/g).…”

“…The methanolic extract obtained from cold percolation extraction by Kumar et al ( 42 ) exhibited scavenging capacities of 28.6–91.4% in a concentration-dependent manner (50–500 μg/ml). The macerated-aqueous extract produced by Ouédraogo et al ( 47 ) gave antioxidant capacity equivalent to ascorbic acid 360.0 mg/g extract.…”

Antiradical and inhibitory activities of hydroethanolic extracts of Phyllanthus amarus and Striga hermonthica on α-amylase

“…Thus, Phyllanthus (P.) amarus Schum & Thonn. and Striga (S.) hermonthica are plants used in traditional medicine throughout the world, particularly by the herbalists of Bobo-Dioulasso in the management of diabetes 10,11,12 . This study has been initiated in order to investigate the free DPPH radicals scavenging and α-amylase inhibitory activities of these plants.…”