Evaluation of the antithrombotic activity of Zhi-Xiong Capsules, a Traditional Chinese Medicinal formula, via the pathway of anti-coagulation, anti-platelet activation and anti-fibrinolysis

Zhou, Jingjing; Song, Zehai; Han, Mingshu; Yu, Bo; Lv, Guanghui; Han, Na; Liu, Zhihui; Yin, Jun

doi:10.1016/j.biopha.2017.11.135

Cited by 17 publications

(12 citation statements)

References 26 publications

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“…In terms of PCA and PLS-DA, after the preadministration of QXH, the same parameters in the QS-BSS model were close to those in the control and CDDP groups, also indicating that QXH has significant pharmacodynamic effect on QS-BSS. All of the above results were consistent with those of previous studies [63][64][65]. Hence, QXH, through promoting blood circulation and removing blood stasis in treating QS-BSS, gives us a glance at TCM compound formula in the treatment of the disease with a variety of pathogenesis by the synergy of various herbal medicines.…”

Section: Discussionsupporting

confidence: 90%

Deciphering the Active Compounds and Mechanisms of Qixuehe Capsule on Qi Stagnation and Blood Stasis Syndrome: A Network Pharmacology Study

Huang

Yue

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Qixuehe capsule (QXH), a Chinese patent medicine, has been demonstrated to be effective in the treatment of menstrual disorders. In traditional Chinese medicine (TCM) theory, qi stagnation and blood stasis syndrome (QS-BSS) is the main syndrome type of menstrual disorders. However, the pharmacodynamic effect of QXH in treating QS-BSS is not clear, and the main active compounds and underlying mechanisms remain unknown. Methods. A rat model of QS-BSS was established to evaluate the pharmacodynamic effect of QXH. Thereafter, a network pharmacology approach was performed to decipher the active compounds and underlying mechanisms of QXH. Results. QXH could significantly reduce the rising whole blood viscosity (WBV) and plasma viscosity (PV) but also normalize prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), and fibrinogen (FIB) content in QS-BSS rats. Based on partial least-squares-discriminant analysis (PLS-DA), the low-dose QXH-intervened (QXH-L) and the high-dose QXH-intervened (QXH-H) groups seemed the most effective by calculating the relative distance to normality. Through network pharmacology, QXH may improve hemorheological abnormality mainly via 185 compounds-51 targets-28 pathways, whereas 184 compounds-68 targets-28 pathways were associated with QXH in improving coagulopathy. Subsequently, 25 active compounds of QXH were verified by UPLC-Q/TOF-MS. Furthermore, 174 active compounds of QXH were shared in improving hemorheological abnormality and coagulopathy in QS-BSS, each of which can act on multiple targets to be mainly involved in complement and coagulation cascades, leukocyte transendothelial migration, PPAR signaling pathway, VEGF signaling pathway, and arachidonic acid metabolism. The attribution of active compounds indicated that Angelicae Sinensis Radix (DG), Paeoniae Radix Rubra (CS), Carthami Flos (HH), Persicae Semen (TR), and Corydalis Rhizoma (YHS) were the vital herbs of QXH in treating QS-BSS. Conclusion. QXH can improve the hemorheology abnormality and coagulopathy of QS-BSS, which may result from the synergy of multiple compounds, targets, and pathways.

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Section: Discussionsupporting

confidence: 90%

Deciphering the Active Compounds and Mechanisms of Qixuehe Capsule on Qi Stagnation and Blood Stasis Syndrome: A Network Pharmacology Study

Huang

Yue

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…In this study, LJ markedly shortened APTT, PT, and TT and inhibited the production of FIB compared to those in the model group. However, these results are inconsistent with previous reports; [40,42] these indexes were significantly shorter than those in the acute blood stasis model compared with the normal rats and were longer after treatment with drugs due to prophylactic administration. Blood coagulation function and fibrinolytic activity are activated when disseminated intravascular coagulation occurs in the body.…”

Section: Methodscontrasting

confidence: 94%

“…[16,40] APTT and PT are related to endogenous and exogenous coagulation pathways, respectively, whereas TT and FIB reflect changes in the common intrinsic and extrinsic coagulation pathways. [8,16,[40][41][42] In this study, LJ markedly shortened APTT, PT, and TT and inhibited the production of FIB compared to those in the model group. However, these results are inconsistent with previous reports; [40,42] these indexes were significantly shorter than those in the acute blood stasis model compared with the normal rats and were longer after treatment with drugs due to prophylactic administration.…”

Section: Discussionmentioning

confidence: 47%

“…Meanwhile, platelets play a vital part in BS, whereas the massive aggregation of platelets may cause pathologic thrombosis. [16,42] In addition, thromboxane A2 and prostaglandins I2 are two mutually antagonistic substances, and the former enhances platelet overaggregation, while the latter promotes vasodilation. [16,40,42] In addition, TXB2 and 6-keto-PGF1α are the metabolites of thromboxane A2 and prostaglandins I2, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, TXB2 and 6‐keto‐PGF1α are the metabolites of thromboxane A2 and prostaglandins I2, respectively. Therefore, the levels of TXB2, 6‐keto‐PGF1α, and TXB2/6‐keto‐PGF1α are three important factors to evaluate the antiplatelet effect [16,42] . In addition, PAI‐1, t‐PA, and u‐PA are three crucial parameters in the fibrinolytic system [16,42] .…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Activating Blood Circulation, Anti‐Inflammatory and Diuretic Effects of Leonurus japonicus Extract on a Rat Model of Trauma Blood Stasis and Its Phytochemical Profiling

Zhang

Nie

Yang

et al. 2023

Chemistry & Biodiversity

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Leonurus japonicus Houtt. has been traditionally used to treat many ailments. This study evaluated the activating blood circulation, anti-inflammatory, and diuretic effects of L. japonicus extract (LJ) and identified its phytochemicals. In this work, the phytochemicals in LJ were identified using liquid chromatography mass spectrometry. Rats were randomly assigned to three groups (n = 8): Control group was treated with saline, while the Model group (saline) and LJ group (426 mg/kg) had induced traumatic injury. All rats were treated with once by daily oral gavage for one week. The biochemical indices and protein expression were measured. Herein, 79 constituents were identified in LJ, which were effective in elevating body weight, food consumption, water intake, and urinary excretion volume, as well as in ameliorating traumatic muscle tissues in model rats. In addition, LJ prominently decreased the contents of plasma viscosity, platelet aggregation rate, thrombin time, prothrombin time, activated partial thromboplastin time, fibrinogen, thromboxane B2 (TXB2), TXB2/6-ketoprostaglandin F1α (6-keto-PGF1α), urokinase-type plasminogen activator (u-PA), plasminogen activator inhibitor 1 (PAI-1), PAI-1/tissue-type PA (t-PA), and PAI-1/u-PA, while significantly increasing antithrombin III, 6-keto-PGF1α, and t-PA contents. Furthermore, LJ notably inhibited tumor necrosis factor alpha, interleukin 6 (IL-6), IL-8, angiotensin II, antidiuretic hormone, aldosterone, aquaporin 1 (AQP1), AQP2, and AQP3 levels, and markedly elevating IL-10 and natriuretic peptide levels. Finally, LJ markedly reduced the protein expression of AQP1, AQP2, and AQP3 compared to the model group. Collectively, LJ possessed prominent activating blood circulation, antiinflammatory, and diuretic effects, thus supporting the clinical application of L. japonicus.

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Chemical profiling and mechanistic studies of Zhi‐Shang‐Feng granules against influenza virus by high‐performance liquid chromatography coupled with Q exactive focus hybrid quadrupole orbitrap high‐resolution mass spectrometry in combination with network pharmacology analysis

Xie,

Luo,

et al. 2023

J of Separation Science

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Zhi‐Shang‐Feng Granules are used in the clinical treatment of influenza to relieve headaches, chills and fever, bronchitis, nasal congestion, neuralgia and other symptoms. To decipher the components responsible for therapeutic effects of Zhi‐Shang‐Feng g ranules against influenza virus, an analytical method based on high‐performance liquid chromatography coupled with Q exactive focus hybrid quadrupole orbitrap high resolution mass spectrometry was developed and the chemical profile of Zhi‐Shang‐Feng granules was characterized. Then, the identified components were used to conduct network pharmacological analysis and determine the potential mechanism of Zhi‐Shang‐Feng Granules. As a result, 177 compounds were putatively identified through comprehensive analysis by liquid chromatography coupled with high‐resolution mass spectrometry, of which 23 compounds were unambiguously confirmed with reference standards. Components in Zhi‐Shang‐Feng Granules were found to specifically act on different enzymes, G‐protein‐coupled receptors, ion channels and transporters in the immune, endocrine, nervous, and circulatory systems. The potential mechanism was related to several biological processes, including cell growth and death, pattern recognition receptor signalling, signalling by interleukins, and lipid metabolism. The combination of chemical profile characterization and network construction provided useful insight into the overall chemical composition of Zhi‐Shang‐Feng granules and revealed their potential anti‐infection, anti‐inflammatory and immunoregulatory mechanisms against influenza virus infected disease.

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Evaluation of the antithrombotic activity of Zhi-Xiong Capsules, a Traditional Chinese Medicinal formula, via the pathway of anti-coagulation, anti-platelet activation and anti-fibrinolysis

Cited by 17 publications

References 26 publications

Deciphering the Active Compounds and Mechanisms of Qixuehe Capsule on Qi Stagnation and Blood Stasis Syndrome: A Network Pharmacology Study

Deciphering the Active Compounds and Mechanisms of Qixuehe Capsule on Qi Stagnation and Blood Stasis Syndrome: A Network Pharmacology Study

Activating Blood Circulation, Anti‐Inflammatory and Diuretic Effects of Leonurus japonicus Extract on a Rat Model of Trauma Blood Stasis and Its Phytochemical Profiling

Chemical profiling and mechanistic studies of Zhi‐Shang‐Feng granules against influenza virus by high‐performance liquid chromatography coupled with Q exactive focus hybrid quadrupole orbitrap high‐resolution mass spectrometry in combination with network pharmacology analysis

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