Evaluation of the Antiviral Activity of Propolis from Native Bees (&amp;lt;i&amp;gt;Plebeia frontalis&amp;lt;/i&amp;gt;) against Canine Distemper Virus

Jiménez, Olga Valeria Domínguez; Pérez, Betsabé Rodríguez; Sánchez, Tonatiuh Alejandro Crúz; Tovar, Carlos Gerardo García; Bordes, José Luis Nieto; Zárate, Carlos Ignacio Soto

doi:10.4236/ojvm.2020.1012018

Cited by 4 publications

(1 citation statement)

References 19 publications

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“…Our team demonstrated the antiviral activity of the propolis of the stingless bee Plebeia frontalis against the canine distemper virus. This encourages further investigation into its effects on other viruses that affect animals [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antimycotic Activity and Structural Damage against Canine Malassezia pachydermatis Strains Caused by Mexican Stingless Bee Propolis

Fuentes Esquivel,

Pérez,

Betancourt

et al. 2024

Veterinary Sciences

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This work describes the antimycotic activity of propolis from the stingless bees Scaptotrigona mexicana and Tetragonisca angustula, collected from two Mexican regions (Veracruz and Chiapas, respectively), against three clinical isolates and the reference strain ATCC 14522 of Malassezia pachydermatis, the causative agent of canine otitis. The chemical components of the ethanolic extracts of propolis were determined by gas chromatography coupled with mass spectrometry (GC-MS), and sesquiterpenes were the predominant compounds. The antimycotic activity was evaluated by plate microdilution. The induced changes in the yeasts were evaluated by fluorescence microscopy and staining with calcofluor white and propidium iodide. The minimum inhibitory concentration (MIC) was 7.11 mg/mL, and the minimum fungicidal concentration was 21.33 mg/mL for both extracts. The EPPs of Scaptotrigona mexicana and Tetragonisca angustula caused substantial damage to yeast morphology, where the propidium iodide staining of the yeasts treated with both EEPs revealed the penetration of this marker, which indicates the destruction of the cell wall and plasma membrane of the fungi. This result suggests that these types of propolis could be used as alternative treatments for canine external otitis. To the best of our knowledge, this seems to be the first scientific report that has demonstrated structural damage in Malassezia pachydermatis by Mexican stingless bee propolis.

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Section: Discussionmentioning

confidence: 99%

In Vitro Antimycotic Activity and Structural Damage against Canine Malassezia pachydermatis Strains Caused by Mexican Stingless Bee Propolis

Fuentes Esquivel,

Pérez,

Betancourt

et al. 2024

Veterinary Sciences

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Activity of Propolis Nanoparticles against HSV-2: Promising Approach to Inhibiting Infection and Replication

et al. 2022

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Herpes simplex type 2 (HSV-2) infection causes a significant life-long disease. Long-term side effects of antiviral drugs can lead to the emergence of drug resistance. Thus, propolis, a natural product derived from beehives, has been proposed to prevent or treat HSV-2 infections. Unfortunately, therapeutic applications of propolis are still limited due its poor solubility. To overcome this, a nanoparticle-based drug delivery system was employed. An ethanolic extract of propolis (EEP) was encapsulated in nanoparticles composed of poly(lactic-co-glycolic acid) and chitosan using a modified oil-in-water single emulsion by using the solvent evaporation method. The produced nanoparticles (EEP-NPs) had a spherical shape with a size of ~450 nm and presented satisfactory physicochemical properties, including positively charged surface (38.05 ± 7.65 mV), high entrapment efficiency (79.89 ± 13.92%), and sustained release profile. Moreover, EEP-NPs were less cytotoxic on Vero cells and exhibited anti-HSV-2 activity. EEP-NPs had a direct effect on the inactivation of viral particles, and also disrupted the virion entry and release from the host cells. A significant decrease in the expression levels of the HSV-2 replication-related genes (ICP4, ICP27, and gB) was also observed. Our study suggests that EEP-NPs provide a strong anti-HSV-2 activity and serve as a promising platform for the treatment of HSV-2 infections.

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Bazi Hastaliklarin Tedavi̇si̇ni̇n Desteklenmesi̇nde Propoli̇s Kullaniminin Önemi̇

Uğur

Temelli²,

Eyigör³

2021

Veteriner Farmakoloji Ve Toksikoloji Derneği Bülteni

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Son yıllarda geleneksel ve tamamlayıcı tıp, apiterapi ve organik ürünlere olan ilgi ve talebin artması ile fonksiyonel bir gıda ürünü olan propolis, alternatif olarak ön plana çıkmış ve bu konuda yapılan araştırmalar artmıştır. Çalışmalarda, propolisin antibakteriyel, antifungal, antiviral, antikanser, antitümör, lokal anestezik, antiinflamatuvar ve antioksidan özellikleri ile kalp ve diyabet gibi kronik hastalıklar, kanser, ağız ve diş hastalıkları, viral hastalıklar, gastrointestinal, nörolojik ve deri hastalıklarında olumlu yönde etkisinin bulunduğu bildirilmiştir. Propolisin standardizasyonu ile ilgili çalışmalar ülkemizde yürütülmekte olup diğer ülkelerde de mevcut çalışmalar içerisinde eksiklikler olmakta ve henüz genel kabul görmüş bir standardizasyon bulunmamaktadır. Bu nedenle, propolisin fonksiyonel özellikleri üzerinde değişen etkiler oluşturması önemli bir sorun olarak karşımıza çıkmaktadır. Ayrıca, doz-yanıt ve yapı-işlev mekanizmasının daha iyi anlaşılmasını sağlayabilecek nitelikte yeni araştırmalara gereksinim duyulmaktadır. Bu derlemede, propolisin bazı hastalıkların tedavisine destek olabilecek ortaya konulmuş faydalı etkilerinin güncel olarak belirlenmesi ve değerlendirilmesi amaçlanmıştır.

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Evaluation of the Antiviral Activity of Propolis from Native Bees (<i>Plebeia frontalis</i>) against Canine Distemper Virus

Cited by 4 publications

References 19 publications

In Vitro Antimycotic Activity and Structural Damage against Canine Malassezia pachydermatis Strains Caused by Mexican Stingless Bee Propolis

In Vitro Antimycotic Activity and Structural Damage against Canine Malassezia pachydermatis Strains Caused by Mexican Stingless Bee Propolis

Activity of Propolis Nanoparticles against HSV-2: Promising Approach to Inhibiting Infection and Replication

Bazi Hastaliklarin Tedavi̇si̇ni̇n Desteklenmesi̇nde Propoli̇s Kullaniminin Önemi̇

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