Evaluation of the anxiolytic activity of the ethanolic extract of bryophyllum pinnatum (kurz) in mice

Amali, Mohammed O.; Atunwa, Soliu Abiola; Aiyelero, Oyeronke Medinat; Oyedotun, Eniola O.

doi:10.1016/j.ibror.2019.09.081

Cited by 1 publication

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“…Furthermore, the elevated plus maze is widely being considered a suitable model for evaluating anxiolytic or anxiogenic potentials of substances in rodents (Amali et al 2019). It was originally developed by Pellow et al (1985) and its principle is largely based on the innate aversion of rodents to heights and open spaces.…”

Section: Discussionmentioning

confidence: 99%

“…In order to obtain the ethanolic extract, 900 g powder of C. micranthum leaves was boiled in 2 L of 95% ethanol under reflux and then extracted with ethanol by maceration for a period of 48 h. Subsequently, the mixture was filtered using Whatman filter paper 1.0. The resultant filtrate was concentrated over the vapour of the water bath at 45 °C and dried under vacuum (Amali et al 2019). The percentage yield of extracts was calculated as:…”

Section: Preparation Of Ethanolic Leaf Extract Of Combretum Micranthummentioning

confidence: 99%

“…This could be because of general perception of their safety and cultural acceptability, widespread availability, costeffectiveness, ease of administration, tolerability and general compliance (Calixto 2000;WHO 2005). Several studies have shown that various ethnomedicinal plants have been documented for the treatment of CNS disorders (Amali et al 2019;Danmalam et al 2017), which underscores the need for continuous research on potential medicinal plants that possess anxiolytic property.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of anxiolytic potential and acute toxicity study of Combretum micranthum G. Don. leaves (Combretaceae)

Amali

Atunwa

Omotesho

et al. 2020

Journal of Medicinal Plants for Economic Development

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Background: Combretum micranthum G. Don. (Combretaceae) is an ethnomedicinally valuable, undomesticated and indigenous shrub of West Africa. However, its anxiolytic potential have not been reported despite its ethanolic extract being used ethnomedicinally in the management of anxiety disorders.Aim: To determine the acute toxicity effect and assess the behavioural effects and anxiolytic potential of C. micranthum G. Don. leaves in mice.Settings: This study is an experimental design to evaluate the ethnomedicinal claim of Combretum micranthum G. Don using animal models of anxiety.Methods: Fifty-six male and female mice, ranging in weight between 20 g and 30 g were randomly distributed into three main groups. The first group of mice (n = 6) was assigned for toxicity assessment (LD 50 ) study using the guideline of Organization for Economic Cooperation and Development (OECD). The second group of mice for behavioural study (n = 25) was further divided into five sub-groups. Sub-groups I, II and III were orally administered 500 mg/kg, 1000 mg/kg, 2000 mg/kg of ethanolic extract of C. micranthum (CmEE), respectively, whilst IV and V were intraperitoneally administered 1 mg/kg diazepam and normal saline 0.5 mL, respectively. They were thereafter evaluated for novelty-induced behaviours: locomotion, rearing and grooming using Open Field Test (OFT). The third group of mice (n = 25) was treated similar to the pattern used in behavioural study and evaluated for anxiolytic activity of CmEE using elevated plus maze (EPM) model. Data were expressed as mean ± standard error of mean (S.E.M) and analysed using Student's-t test, and one-way analysis of variance (ANOVA) followed by Student-Newman-Keuls (SNK) test with values of p < 0.05 considered significant. Results:The percentage yield of ethanolic leaf extract of C. micranthum was 14.28% weight/ weight (w/w). Combretum micranthum showed no toxicity when administered orally to mice (LD 50 ≥ 2000 mg/kg). Groups administered 500, 1000 and 2000 mg/kg of CmEE exhibited decreased locomotion (p < 0.05) when compared with saline group. There was significant decrease in rearing at 2000 mg/kg but increase in grooming in mice administered 2000 mg/kg of CmEE was recorded. The groups administered 500, 1000 and 2000 mg/kg of CmEE showed increased percentage time spent in the open arm in a dose-dependent pattern (33.3%, 41.6% and 55.4%, respectively) when compared with the saline group. There were significant dosedependent decreases in the indices of open arm avoidance at 1000 (48.9) and 2000 mg/kg (41.4) of CmEE. Conclusion:Combretum micranthum is non-toxic and preliminary data indicated that it possesses anxiolytic potential. However, it is recommended that further assays using other specific models of anxiety to determine its probable mechanism(s) of action should be explored.

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Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Ethanolic Leaf Extract Of Combretum Micranthummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of anxiolytic potential and acute toxicity study of Combretum micranthum G. Don. leaves (Combretaceae)

Amali

Atunwa

Omotesho

et al. 2020

Journal of Medicinal Plants for Economic Development

View full text Add to dashboard Cite

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Evaluation of the anxiolytic activity of the ethanolic extract of bryophyllum pinnatum (kurz) in mice

Cited by 1 publication

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Assessment of anxiolytic potential and acute toxicity study of Combretum micranthum G. Don. leaves (Combretaceae)

Assessment of anxiolytic potential and acute toxicity study of Combretum micranthum G. Don. leaves (Combretaceae)

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