Evaluation of the API Coryne Test System for Identification of <i>Actinomyces pyogenes</i>

Ding, H.; Lämmler, Christoph

doi:10.1111/j.1439-0450.1992.tb01168.x

Cited by 14 publications

(13 citation statements)

References 13 publications

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“…The biochemical properties of the seven A. haemolyticum strains determined with the API Coryne test system are summarized in Table 2. The results corresponded to previous findings (10). The accuracy of the API Coryne test system for identification of A. haemolyticum has already been demonstrated (11,12).…”

Section: Resultssupporting

confidence: 80%

“…The A. haemolyticum cultures were additionally cultivated on Loeffler agar (2) and for determination of cross-reactions with streptococcal serogroup G-specific antisera investigated with a commercial grouping kit (streptococcal identification kit; Oxoid, Wesel, Germany). For biochemical characterization, the API Coryne test system (Biomerieux, Nürtingen, Germany) was used according to the instructions of the manufacturer as described previously (10). In addition, tablets containing substrates for investigation of the enzymes alkaline phosphatase, ␣-mannosidase, and pyrrolidonyl arylamidase (Inverness Medical, Köln, Germany) were used according to the instructions of the manufacturer (Rosco Diagnostica A/S, Taastrup, Denmark).…”

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confidence: 99%

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Phenotypic and Genotypic Characterization of Arcanobacterium haemolyticum Isolates from Infections of Horses

et al. 2009

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The present study was designed to characterize phenotypically and genotypically seven Arcanobacterium haemolyticum strains obtained from infections of six horses. All seven strains showed the cultural and biochemical properties typical of A. haemolyticum and were susceptible to most of the antibiotics tested. The species identification could be confirmed by amplification and sequencing of the 16S rRNA gene and the 16S-23S rRNA intergenic spacer region and by PCR amplification of species-specific parts of the gene encoding phospholipase D in A. haemolyticum. Use of the latter could possibly improve future identification of this generally human pathogenic bacterial species which, according to the present results, seems to occur also in infections of horses.Arcanobacterium haemolyticum, originally known as Corynebacterium haemolyticum, was first described in 1946 and reported to be the cause of exudative pharyngitis and skin infections in humans (23). This bacterial species, formerly considered to be a mutant form of Actinomyces (Corynebacterium) pyogenes (1), has been reclassified as a single species in the newly proposed genus Arcanobacterium (8). In the following years, the mainly animal pathogenic species Actinomyces pyogenes and human pathogenic Actinomyces bernardiae were renamed Arcanobacterium pyogenes and Arcanobacterium bernardiae, respectively, and five new species, namely, Arcanobacterium bialowiezense, Arcanobacterium bonasi, Arcanobacterium hippocoleae, Arcanobacterium phocae, and Arcanobacterium pluranimalium, were assigned to this genus (14,19,20,28). A. haemolyticum, known as a pathogen causing nonstreptococcal pharyngitis (6, 22), could also be isolated from systemic and deep-seated infections of humans. The latter was reviewed by Skov et al. (31) and Tan et al. (33). However, the isolation of A. haemolyticum from animals appears to be rare. Tyrrell et al. (34) discussed the etiological role of a single A. haemolyticum strain isolated from a periodontal infection of a rabbit.The aim of the present study was to characterize phenotypically and genotypically seven A. haemolyticum strains isolated from infections of six horses over a period of 10 years. MATERIALS AND METHODSBacterial cultures. A total of 21 bacterial cultures were used in this study. The cultures included the reference strains A. haemolyticum DSM 20595, A. bernardiae DSM 9152, A. bialowiezense DSM 17162, A. bonasi DSM 17163, A. hippocoleae DSM 15539, A. phocae DSM 10002, A. pluranimalium DSM 13483, A. pyogenes DSM 20630, Corynebacterium pseudotuberculosis biovar equi DSM 7177, and C. pseudotuberculosis biovar ovis DSM 7179 and seven bacterial cultures isolated from infections of six horses which were presumptively identified as A. haemolyticum. Further data about the origins of the seven strains are summarized in Table 1.Phenotypic properties. Hemolytic properties of the bacteria were examined on blood agar containing 5% sheep or rabbit blood after incubation of the bacteria for 24 to 48 h in a candle jar. The rabbit blood was ob...

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Section: Resultssupporting

confidence: 80%

mentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Arcanobacterium haemolyticum Isolates from Infections of Horses

et al. 2009

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“…Two studies (27,39) have examined the ability of the API Coryne system (bioMerieux-Vitek) to identify strains of A.…”

Section: Gram-negative Bacillimentioning

confidence: 99%

“…Ding and Lammler (27) determined that 36 of 42 A. pyogenes strains were correctly identified with the API Coryne system, with the remaining six strains yielding unlisted profile numbers. These workers concluded that while the system facilitated identification of A. pyogenes, an improved data base was needed to enhance accuracy.…”

Section: Gram-negative Bacillimentioning

confidence: 99%

“…Commercial identification systems are widely used by clinical microbiologists as convenient, cost-effective alternatives to conventional methods. However, many of these systems incorporate a limited number of veterinary strains in their data base which may limit the accuracy of the system for identification of veterinary pathogens (27,59,60,85,97,102,104,105,(111)(112)(113). A review of the commercial systems that have been evaluated with veterinary pathogens follows.…”

Section: Introductionmentioning

confidence: 99%

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Identification of veterinary pathogens by use of commercial identification systems and new trends in antimicrobial susceptibility testing of veterinary pathogens

Watts

Yancey

1994

Clin Microbiol Rev

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Veterinary diagnostic microbiology is a unique specialty within microbiology. Although isolation and identification techniques are similar to those used for human pathogens, many veterinary pathogens require unique cultivation or identification procedures. Commercial identification systems provide rapid, accurate identification of human pathogens. However, the accuracy of these systems with veterinary pathogens varies widely depending on the bacterial species and the host animal from which it was isolated. Increased numbers of veterinary strains or species in the data bases of the various systems would improve their accuracy. Current procedures and interpretive criteria used for antimicrobial susceptibility testing of veterinary pathogens are based on guidelines used for human pathogens. The validity of these guidelines for use with veterinary pathogens has not been established. As with fastidious human pathogens, standardized methodologies and quality control isolates are needed for tests of organisms such as Actinobacillus pleuropneumoniae and Haemophilus somnus. Furthermore, interpretive criteria for veterinary antimicrobial agents based on the MIC for veterinary pathogens, the pharmacokinetics of the antimicrobial agent in the host animal, and in vivo efficacy of the antimicrobial agent are needed. This article reviews both the commercial identification systems evaluated with veterinary pathogens and current methods for performing and interpreting antimicrobial susceptibility tests with veterinary pathogens. Recommendations for future improvements in both areas are discussed.

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A fatal case of Arcanobacterium pyogenes endocarditis in a man with no identified animal contact: case report and review of the literature

Plamondon

Martínez

Raynal

et al. 2007

Eur J Clin Microbiol Infect Dis

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Evaluation of the API Coryne Test System for Identification of Actinomyces pyogenes

Cited by 14 publications

References 13 publications

Phenotypic and Genotypic Characterization of Arcanobacterium haemolyticum Isolates from Infections of Horses

Phenotypic and Genotypic Characterization of Arcanobacterium haemolyticum Isolates from Infections of Horses

Identification of veterinary pathogens by use of commercial identification systems and new trends in antimicrobial susceptibility testing of veterinary pathogens

A fatal case of Arcanobacterium pyogenes endocarditis in a man with no identified animal contact: case report and review of the literature

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