Evaluation of the associations between GDF-15, OPG, and IL-15 levels in CRC: preliminary results

Abstract

Introduction: Osteoprotegerin (OPG), , and Growth/Differentiation are all proven to take part in the processes associated with colorectal carcinoma (CRC) including tissue remodeling, inflammation modulation, and metastasis.Aim of the study: To investigate the concentrations of GDF-15, OPG and IL-15 in tumor and margin tissues of CRC in relation to clinicopathological features of patients. Material and methods:The study used 50 specimens of tumor and tumor-free margin tissues obtained from CRC patients. To determine the GDF-15, OPG and IL-15 concentrations commercially available enzyme-linked immunosorbent assay (ELISA) kits were used.Results: Concentrations of GDF-15, OPG and IL-15 were significantly higher in the tumor in comparison with the margin. The tumor levels of GDF-15 were positively associated with those of OPG and IL-15, while tumor levels of OPG correlated positively with those of IL-15. There was no association between levels of investigated molecules and clinical features of patients. Conclusions:The levels of GDF-15, OPG and IL-15 are elevated in patients suffering from CRC. More studies are needed to establish a specific role of these cytokines in the development, tumor growth, progression, and prognosis of CRC and to examine their role as possible CRC treatment candidates.

Magneto-controlled electrochemical immunosensing platform to assess the senescence-associated GDF-15 marker in colorectal cancer

“…54,55 These results are perfectly in line with the valuable potential of GDF-15 for CRC detection and prognosis. 14,17,20,21,22,47,[56][57][58][59][60] Indeed, it has been reported that GDF-15 levels in serum gradually increase in the process of conversion from adenomatous polyps to colorectal carcinoma 14,59 and that CRC patients with an increased serum level of GDF-15 had a 2.09-fold higher risk of death 11 and worse cancer-specific survival (CSS). 21 In our results, this would correspond to the CRC plasma samples at advanced stages (1.85-fold higher GDF-15 plasma values than healthy individuals).…”

“…19,20 Therefore, GDF-15 dysregulation within the SASP framework underlines its relevance as a well-established biomarker for detecting and characterizing metastatic CRC. 15,21,22…”

“…19,20 Therefore, GDF-15 dysregulation within the SASP framework underlines its relevance as a well-established biomarker for detecting and characterizing metastatic CRC. 15,21,22 Considering the importance of GDF-15 in assessing the presence and progression of CRC, clinical research strives to develop simple, sensitive, accurate, and cost-effective methods to monitor this biomarker. Traditionally, immunohistochemistry 23 and blotting technologies 24 have been used for GDF-15 detection, deprived of quantitative ability.…”