Evaluation of the Bactec microbial detection system for culturing miscellaneous sterile body fluids

Akçam, Füsun Zeynep; Yayli, Güler; Uşkun, Ersin; Kaya, Onur; Demir, Canan

doi:10.1016/j.resmic.2005.10.005

Cited by 38 publications

(30 citation statements)

References 11 publications

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“…From the laboratory side, the higher cost associated with automated systems is a potential drawback of their routine use. 3,4,5,14 However, the earlier and higher yield obtained with these systems may have a positive impact on patient care and shorten hospital stay.…”

Section: Discussionmentioning

confidence: 99%

“…[3][4][5]8 Decisions regarding which culture method to use should be individualised by taking factors like clinical patient information, laboratory resources and total cost into account. In our study population, adult patients as a group derived specific benefit from the use of the Bactec method, as they appear to be the group most at risk of infection with fastidious organisms like N. gonorrhoeae.…”

Section: Discussionmentioning

confidence: 99%

“…However, culture of synovial fluid in patients with suspected septic arthritis is frequently disappointing and a causative organism is only recovered in one-to two-thirds of cases. [3][4][5][6][7][8][9][10] Factors contributing to this problem include prior administration of antibiotics, inappropriate specimen collection procedures, delayed transport to the laboratory and suboptimal isolation techniques. [3][4][5][6][7][8][9][10] The yield of conventional agar-based culture methods is limited due to the low volume of fluid cultured, which necessitates concentration of the fluid prior to inoculation of media.…”

Section: Introductionmentioning

confidence: 99%

“…[3][4][5][6][7][8][9][10] Factors contributing to this problem include prior administration of antibiotics, inappropriate specimen collection procedures, delayed transport to the laboratory and suboptimal isolation techniques. [3][4][5][6][7][8][9][10] The yield of conventional agar-based culture methods is limited due to the low volume of fluid cultured, which necessitates concentration of the fluid prior to inoculation of media. 4,5,7 Furthermore, overgrowth by contaminating microflora, the bacteriostatic effect of purulent exudates on bacterial growth, phagocytosis of microorganisms by pus cells in the fluid and the inherent fastidious nature of some pathogens may be responsible for false negative cultures.…”

Section: Introductionmentioning

confidence: 99%

“…6 A number of studies have found that culture of synovial fluid in blood culture systems is superior to conventional culture methods. [3][4][5][6]9,11,12 In some studies, fastidious organism supplement (FOS) was added to blood culture bottles to optimise the recovery of fastidious bacteria such as Neisseria and Haemophilus species. 5 FOS, a product of Becton Dickinson Diagnostic Instrument Systems, was designed to neutralise the toxic effects exerted by sodium polyanethol sulfonate, present in the Bactec blood culture bottles in concentrations of up to 0.05 %.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Superior detection of pathogens in synovial fluid by the Bactec 9240 Peds Plus/F system compared to the conventional agar-based culture method

Nutt

Orth

Goodway

et al. 2010

Southern African Journal of Epidemiology and Infection

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Superior detection of pathogens in synovial fluid by the Bactec 9240 Peds Plus/F system compared to the conventional agar-based culture method

Nutt

Orth

Goodway

et al. 2010

Southern African Journal of Epidemiology and Infection

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Correlation of microbiologic culture and fine‐needle aspiration cytology: A 14‐year experience at a single institution

Clement

Williams‐Bouyer

Nawgiri

et al. 2015

Cancer Cytopathology

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identified by cytology and culture, 44 (34%) were identified by culture only, and 22 (17%) were identified by cytology only. Among the 67 cases of fungi, 34 (51%) were identified by cytology only, with 15 of these 34 cases being fungal hyphae; 25 cases (37%) were identified by cytology and culture, with a 100% concordance between the cytology diagnosis and culture result; and 8 cases (12%) were identified by culture only. CONCLUSIONS: FNA cytology-culture correlation is a valuable tool with which to assess the efficacy and limitations of the direct diagnosis of infectious agents, and to identify types of infections that may be negative on culture but positive on cytology diagnosis. Cancer (Cancer Cytopathol) 2015;123:612-9.

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Comparison of the BACTEC blood culture system versus conventional methods for culture of normally sterile body fluids

et al. 2007

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This study compared the BACTEC blood culture system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md) with conventional culture methods for recovery and time to detection of significant isolates from normally sterile body fluids. A total of 412 specimens were included in the study. Half of the specimens were inoculated directly into the automated blood culture system. The remaining specimens were centrifuged at 3000 rpm for 10 min and were inoculated onto conventional media. Clinically significant microorganisms were isolated from 41 specimens (10%) by both culture systems; however, for 62 specimens (14.9%), growth was detected only with the BACTEC system. No isolates were detected with only conventional culture methods. A significant difference was noted between the blood culture system and routine culture methods for recovery of pathogenic microorganisms that were from sterile body fluids. The most frequently isolated microorganisms recovered only with the blood culture system were gram-positive cocci; gram-negative bacilli were the most frequently isolated microorganisms that were recovered with both culture methods. Streptococcus pneumoniae, Streptococcus viridans, Aeromonas hydrophila, and Brucella were recovered only with the blood culture system. Furthermore, the mean time to detection of significant pathogens was significantly less with the blood culture system than with conventional media. The BACTEC blood culture system was found to improve the yield of clinically significant isolates from normally sterile body fluids with reduced time to detection; it may be advantageous for isolation of fastidious microorganisms, such as Brucella and S pneumoniae, especially from cerebrospinal and synovial fluid specimens.

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Evaluation of the Bactec microbial detection system for culturing miscellaneous sterile body fluids

Cited by 38 publications

References 11 publications

Superior detection of pathogens in synovial fluid by the Bactec 9240 Peds Plus/F system compared to the conventional agar-based culture method

Superior detection of pathogens in synovial fluid by the Bactec 9240 Peds Plus/F system compared to the conventional agar-based culture method

Correlation of microbiologic culture and fine‐needle aspiration cytology: A 14‐year experience at a single institution

Comparison of the BACTEC blood culture system versus conventional methods for culture of normally sterile body fluids

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