Evaluation of the BAX® System for Detection of Listeria monocytogenes in Foods: Collaborative Study

Silbernagel, Karen M; Jechorek, Robert P.; Barbour, W. Mark; Mrozinski, Peter; Alejo, W; Aleo, V; Andaloro, B; Beacorn, F; Benzinger, J; Bogar, S; Brayman, C; Broom, J; Carson, M; Carver, C; Cheng, C; Centrella, B; Clayborn, J; Collins, C; Deibel, C; Divine, M; Eliasberg, S; Farmer, D; Frye, S; Gatesy, T; Goodstein, E; Halker, C; Hall, G; Hanson, P; Hartman, G; Heddaeus, K; Hembree, J; Hutchins, J; Istafanos, P; Jechorek, R; Jenkins, J; Kerdahi, K; Kremer, S; Lal, A; Leighton, S; Lester, D; Lewis, J; Lin, J; Martin, J; Maselli, M; McCarthy, P; McGovern, B; Mills, M; Mohnke, F; Moon, B; Moss, D; Plaza, M; Robeson, S; Romero, H; Rubalcaba, D; Schultz, A; Seehusen, J; Shaw, C; Siem, K; Sloan, E; Stanerson, J; Stepanova, N; Van, K; Enkenvoort, K Van; Vialpando, M; Warren, W; Watts, K; Wilson, K; Woodruff, T

doi:10.1093/jaoac/87.2.395

Cited by 16 publications

(7 citation statements)

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“…A variety of conventional and rapid methods are currently available for the identification and detection of L. monocytogenes in samples, including food, feed and primary production samples, and specimens from listeriosis (Dailey et al ., ). The traditional culture‐based methods remain the ‘gold standard’ for L. monocytogenes detection against which other methods are available, while they are time‐consuming and labor‐intensive, making it unacceptable for rapidly detecting causative pathogens associated with sporadic and outbreaks cases (Aznar & Alarcon, ; Silbernagel et al ., ). A number of PCR, real‐time PCR, and LAMP methods were reported for species‐specific detection of L. monocytogenes , and the primer sequences are mainly based on two gene sequences: hlyA or iap (Liu et al ., ; Tang et al ., ; Wang et al ., , ).…”

Section: Discussionmentioning

confidence: 97%

Rapid and sensitive detection ofListeria monocytogenesby cross-priming amplification oflmo0733gene

Wang

et al. 2014

FEMS Microbiol Lett

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Listeria monocytogenes is a food-borne pathogen that causes severe opportunistic infection in humans and animals. This study reports the development of single cross-priming amplification (S-CPA) and double CPA (D-CPA) assays targeting species-specific gene lmo0733 for identifying L. monocytogenes strains. The CPA assays were performed at a constant temperature 64 °C using seven specific primers and evaluated for specificity and sensitivity. The color change of positive amplification was directly observed by Loopamp Fluorescent Detection Reagent (FD), and the DNA products were visualized as a ladder-like banding pattern on 2.5% gel electrophoresis. Moreover, the positive reactions were also detected by real-time measurement of turbidity. 50 L. monocytogenes and 46 non-L. monocytogenes strains were used for the method verification, and the specificity was 100%. The limit of detection (LoD) of the S-CPA and D-CPA assays was 2.5 pg DNA per reaction and 10-fold more sensitive than PCR. A total of 60 pork samples were tested for L. monocytogenes using the S-CPA assay developed in the study, and the accuracy of the S-CPA and the culture-biotechnical method was 100% identical. The results suggested that the S-CPA assay was a rapid, sensitive, and valuable tool for detection of L. monocytogenes in food products.

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Section: Discussionmentioning

confidence: 97%

Rapid and sensitive detection ofListeria monocytogenesby cross-priming amplification oflmo0733gene

Wang

et al. 2014

FEMS Microbiol Lett

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“…Moreover, the enrichment steps are bacteria-dependent and vary among different laboratories and food types. For instance, various methods to detect L. monocytogenes have been developed by the U.S. Department of Agriculture-Food Safety Inspection Service (USDA-FSIS), Food and Drugs Administration (FDA), and Netherlands Government Food Inspection Services (NGFIS) for all foods (Lammerding and Doyle 1989;van Netten et al 1989;Donnelly 2001;Wallace et al 2003;Kells and Gilmour 2004;Silbernagel et al 2004). In addition, the use of DNA-DNA or DNA-RNA hybridization to determine the presence of certain virulence genes is an alternative approach for detecting bacterial toxins.…”

Section: Microbial Contaminantsmentioning

confidence: 99%

Rapid testing methods for food contaminants and toxicants

Chiou

Leung

Lee

et al. 2015

Journal of Integrative Agriculture

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Food Safety is one of the major concerns in every country regardless of the economic and social development. The frequent occurrence of food scandals in the world has led the Chinese government to implement several strategies to fortify the food supply system to a high food safety standard. This relies heavily on laboratory testing services but conventional methods for detection of food contaminants and toxicants are limited by sophisticated sample preparation procedures, long analysis time, large instruments and professional personnel to meet the increasing demands. In this review, we have incorporated most of the current and potential rapid detection methods for many notorious food contaminants and toxicants including microbial agents, toxic ions, pesticides, veterinary drugs and preservatives, as well as detection of genetically modified food genes and adulterated edible oil. Development of rapid, accurate, easyto-use and affordable testing methods could urge food handlers and the public to actively screen for food contaminants and toxicants instead of passively relying on monitoring by the government examination facility. This review also provides several recommendations including how to encourage the public to engage in the food safety management system and provide optimal education and financial assistance that may improve the current Chinese food safety control system.

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“…It is used to increase sensitivity and specificity and has been used for the identification of L. monocytogenes in clinical samples [57,107–110], environmental samples [78], and milk samples [97,111,112]. Commercially available PCR tests are the BAX® PCR system (Qualicon, Wilmington, DE, USA) and the Probelia® assay (Sanofi Diagnostic Pasteur, Marne la Coquette, France), which have been trialed in the field on a variety of different sample types [113–122].…”

Section: Identification Of Isolated Culturesmentioning

confidence: 99%

Methods for the isolation and identification ofListeriaspp. andListeria monocytogenes: a review

Hughes²,

2005

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Listeria monocytogenes is an important food-borne pathogen and is widely tested for in food, environmental and clinical samples. Identification traditionally involved culture methods based on selective enrichment and plating followed by the characterization of Listeria spp. based on colony morphology, sugar fermentation and haemolytic properties. These methods are the gold standard; but they are lengthy and may not be suitable for testing of foods with short shelf lives. As a result more rapid tests were developed based on antibodies (ELISA) or molecular techniques (PCR or DNA hybridization). While these tests possess equal sensitivity, they are rapid and allow testing to be completed within 48 h. More recently, molecular methods were developed that target RNA rather than DNA, such as RT-PCR, real time PCR or nucleic acid based sequence amplification (NASBA). These tests not only provide a measure of cell viability but they can also be used for quantitative analysis. In addition, a variety of tests are available for sub-species characterization, which are particularly useful in epidemiological investigations. Early typing methods differentiated isolates based on phenotypic markers, such as multilocus enzyme electrophoresis, phage typing and serotyping. These phenotypic typing methods are being replaced by molecular tests, which reflect genetic relationships between isolates and are more accurate. These new methods are currently mainly used in research but their considerable potential for routine testing in the future cannot be overlooked.

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Evaluation of the BAX® System for Detection of Listeria monocytogenes in Foods: Collaborative Study

Cited by 16 publications

References 0 publications

Rapid and sensitive detection ofListeria monocytogenesby cross-priming amplification oflmo0733gene

Rapid and sensitive detection ofListeria monocytogenesby cross-priming amplification oflmo0733gene

Rapid testing methods for food contaminants and toxicants

Methods for the isolation and identification ofListeriaspp. andListeria monocytogenes: a review

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