Evaluation of the Biocontrol Efficiency of Bacillus subtilis Wettable Powder on Pepper Root Rot Caused by Fusarium solani

Qiao, Junqing; Peng, Biao; Zhang, Rongsheng; Liu, Yongfeng; Liu, Youzhou

doi:10.3390/pathogens12020225

Cited by 16 publications

(6 citation statements)

References 35 publications

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“…Currently, several strains of Bacillus spp. are used as biocontrol agents against crop diseases ( Bonaterra et al, 2022 ; Qiao et al, 2023 ; Idris et al, 2024 ). M. esculenta is an important edible fungus that has high economic, nutritional, and medicinal value.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol potential of endophytic Bacillus subtilis A9 against rot disease of Morchella esculenta

Chen,

Zhang,

Chao

et al. 2024

Front. Microbiol.

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IntroductionMorchella esculenta is a popular edible fungus with high economic and nutritional value. However, the rot disease caused by Lecanicillium aphanocladii, pose a serious threat to the quality and yield of M. esculenta. Biological control is one of the effective ways to control fungal diseases.Methods and resultsIn this study, an effective endophytic B. subtilis A9 for the control of M. esculenta rot disease was screened, and its biocontrol mechanism was studied by transcriptome analysis. In total, 122 strains of endophytic bacteria from M. esculenta, of which the antagonistic effect of Bacillus subtilis A9 on L. aphanocladii G1 reached 72.2% in vitro tests. Biological characteristics and genomic features of B. subtilis A9 were analyzed, and key antibiotic gene clusters were detected. Scanning electron microscope (SEM) observation showed that B. subtilis A9 affected the mycelium and spores of L. aphanocladii G1. In field experiments, the biological control effect of B. subtilis A9 reached to 62.5%. Furthermore, the transcritome profiling provides evidence of B. subtilis A9 bicontrol at the molecular level. A total of 1,246 differentially expressed genes (DEGs) were identified between the treatment and control group. Gene Ontology (GO) enrichment analysis showed that a large number of DEGs were related to antioxidant activity related. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the main pathways were Nitrogen metabolism, Pentose Phosphate Pathway (PPP) and Mitogen-Activated Protein Kinases (MAPK) signal pathway. Among them, some important genes such as carbonic anhydrase CA (H6S33_007248), catalase CAT (H6S33_001409), tRNA dihydrouridine synthase DusB (H6S33_001297) and NAD(P)-binding protein NAD(P) BP (H6S33_000823) were found. Furthermore, B. subtilis A9 considerably enhanced the M. esculenta activity of Polyphenol oxidase (POD), Superoxide dismutase (SOD), Phenylal anineammonia lyase (PAL) and Catalase (CAT).ConclusionThis study presents the innovative utilization of B. subtilis A9, for effectively controlling M. esculenta rot disease. This will lay a foundation for biological control in Morchella, which may lead to the improvement of new biocontrol agents for production.

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Section: Discussionmentioning

confidence: 99%

Biocontrol potential of endophytic Bacillus subtilis A9 against rot disease of Morchella esculenta

Chen,

Zhang,

Chao

et al. 2024

Front. Microbiol.

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“…Bacillus species, in particular, have garnered significant research attention for advancing future biological control strategies (Shafi et al, 2017;Etesami et al, 2023;Poulaki and Tjamos, 2023). Currently, various antagonistic Bacillus species have been isolated and screened from soil, plants, and other environments, including B. amyloliquefaciens (Zheng et al, 2018;Wu et al, 2019;Jia et al, 2023;Markovićet al, 2023), B. velezensis (Reyes-Estebanez et al, 2020Chen et al, 2022;Yan et al, 2022), B. subtilis (Bais et al, 2004;Ding et al, 2017;Qiao et al, 2023), among others. Previous investigations highlight the inhibitory and anti-infection effects of B. amyloliquefaciens against pathogenic fungi causing diseases in rice, tomato, potato, brinjal and fruit (Jiao et al, 2020;Nie et al, 2022;Markovićet al, 2023;Pradhan et al, 2023;Zhang et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Bacillus amyloliquefaciens BA-4 and its biocontrol potential against Fusarium-related apple replant disease

Li,

He,

Guo

et al. 2024

Front. Plant Sci.

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Apple replant disease (ARD), caused by Fusarium pathogens, is a formidable threat to the renewal of apple varieties in China, necessitating the development of effective and sustainable control strategies. In this study, the bacterial strain BA-4 was isolated from the rhizosphere soil of healthy apple trees in a replanted orchard, demonstrating a broad-spectrum antifungal activity against five crucial apple fungal pathogens. Based on its morphology, physiological and biochemical traits, utilization of carbon sources, and Gram stain, strain BA-4 was tentatively identified as Bacillus amyloliquefaciens. Phylogenetic analysis using 16S rDNA and gyrB genes conclusively identified BA-4 as B. amyloliquefaciens. In-depth investigations into B. amyloliquefaciens BA-4 revealed that the strain possesses the capacity to could secrete cell wall degrading enzymes (protease and cellulase), produce molecules analogous to indole-3-acetic acid (IAA) and siderophores, and solubilize phosphorus and potassium. The diverse attributes observed in B. amyloliquefaciens BA-4 underscore its potential as a versatile microorganism with multifaceted benefits for both plant well-being and soil fertility. The extracellular metabolites produced by BA-4 displayed a robust inhibitory effect on Fusarium hyphal growth and spore germination, inducing irregular swelling, atrophy, and abnormal branching of fungal hyphae. In greenhouse experiments, BA-4 markedly reduced the disease index of Fusarium-related ARD, exhibiting protective and therapeutic efficiencies exceeding 80% and 50%, respectively. Moreover, BA-4 demonstrated plant-promoting abilities on both bean and Malus robusta Rehd. (MR) seedlings, leading to increased plant height and primary root length. Field experiments further validated the biocontrol effectiveness of BA-4, demonstrating its ability to mitigate ARD symptoms in MR seedlings with a notable 33.34% reduction in mortality rate and improved biomass. Additionally, BA-4 demonstrates robust and stable colonization capabilities in apple rhizosphere soil, particularly within the 10-20 cm soil layer, which indicates that it has long-term effectiveness potential in field conditions. Overall, B. amyloliquefaciens BA-4 emerges as a promising biocontrol agent with broad-spectrum antagonistic capabilities, positive effects on plant growth, and strong colonization abilities for the sustainable management of ARD in apple cultivation.

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“…tritici TSO2T as a promising biocontrol agent against Fusarium languescens , the causative agent of Fusarium wilt in chilies. Qiao et al (2023) reported significant biocontrol activity of Bacillus subtilis PTS-394 against Fusarium solani .…”

Section: Chili Disease Management Using Biocontrol Agentmentioning

confidence: 99%

“… Montoya-Martínez et al (2023) Bacillus subtilis PTS-394 Fusarium solani B. subtilis PTS-394 shown a good biocontrol activity against Fusarium solani at 63 % and 74.43 %. Qiao et al (2023) Bacillus cereus F-BC26 and Bacillus thuringiensis F-BT24 Xanthomonas euvesicatoria F-BT24 and F-BC26 strains formulations promoted Chilies growth and protected it against Xanthomonas euvesicatoria , a causative agent of bacterial spot disease. Hernández-Huerta et al (2023) Bacillus spp.…”

Section: Chili Disease Management Using Biocontrol Agentmentioning

confidence: 99%

Exploring the Global Trends of Bacillus, Trichoderma and Entomopathogenic Fungi for Pathogen and Pest Control in Chili Cultivation

Sam-on,

Mustafa,

Yusof

et al. 2024

Saudi Journal of Biological Sciences

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Evaluation of the Biocontrol Efficiency of Bacillus subtilis Wettable Powder on Pepper Root Rot Caused by Fusarium solani

Cited by 16 publications

References 35 publications

Biocontrol potential of endophytic Bacillus subtilis A9 against rot disease of Morchella esculenta

Biocontrol potential of endophytic Bacillus subtilis A9 against rot disease of Morchella esculenta

Characterization of Bacillus amyloliquefaciens BA-4 and its biocontrol potential against Fusarium-related apple replant disease

Exploring the Global Trends of Bacillus, Trichoderma and Entomopathogenic Fungi for Pathogen and Pest Control in Chili Cultivation

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