Evaluation of the Captia enzyme immunoassays for detection of immunoglobulins G and M to Treponema pallidum in syphilis

Abstract

Two new enzyme-linked immunosorbent assays (ELISA), one for the measurement of immunoglobulin G (IgG) (Captia Syphilis-G) and one for the measurement of IgM (Captia Syphilis-M), were evaluated for detecting antibodies to Treponema pallidum. Serum samples from 169 patients, 96 with various stages of untreated syphilis, 63 with treated syphilis, and 10 who were noninfected, were investigated. Ail sera were also examined by traditional treponemal and cardiolipin tests and by the fluorescent treponemal antibody absorption (FTA-ABS) test for 19S(IgM). The overall sensitivity of Captia Syphilis-G was 98.3%. The IgG ELISA was very sensitive (100%) in all stages of untreated syphilis, except in primary syphilis (82%). In ail diagnostic groups of syphilis, the reactivity of Captia Syphilis-M was similar to that of the 19S(IgM) FTA-ABS test, except in reinfections, in which the IgM capture ELISA was less sensitive. False-positive IgM capture ELISA results were not found in the 10 neonates born to mothers adequately treated for syphilis. However, of six serum samples containing rheumatoid factor, two were reactive in the Captia Syphilis-M test but not in the

Evaluation of a New Competitive Immunoassay (BioElisa Syphilis) for Screening for Treponema pallidum Antibodies at Various Stages of Syphilis

“…The sensitivity of the competitive EIA for both treated and untreated syphilis is similar to that reported for other EIAs for T. pallidum. Lefevre et al (10) obtained 100% sensitivity with the Captia Syphilis G assay, similar to those obtained with TPHA and the FTA-abs test, at all stages of the disease except primary syphilis, in which the EIA may have lower sensitivity. We found that the BioElisa Syphilis detected all five cases of primary syphilis, whereas the TPHA results were still at or below threshold values.…”

Comparison of “Lumipulse anti‐Treponema pallidum” and “Architect Syphilis TP” and further examination

“…Lefevre and colleagues showed that for the detection of anti‐TP antibody for each stage of syphilis, anti‐TP IgM was positive for 16/17 cases of primary syphilis, 11/13 of secondary syphilis, 9/14 of early latent syphilis, and 0/33 of late latent syphilis. On the other hand, anti‐TP IgG was positive for 14/17, 13/13, 14/14, and 33/33 of samples at each stage …”

Reactivity of recombinant treponema pallidum (r‐Tp) antigens with anti‐Tp antibodies in human syphilitic sera evaluated by ELISA

“…2, 3) for G15, five samples (nos. [1][2][3][4]11) for M47, and three samples (nos. 1,2,22) for S42 had a Cl of < 1.0.…”

“…Clinical diagnostics issues related to syphilis and the latest methods for the detection of syphilis antibodies in human blood by immunoassay, immunoblotting, and polymerase chain reaction (PCR) have been reviewed briefly (1). Among the existing immunological methods, we can distinguish two common confirmatory treponemal tests: the agglutination format such as the T. pallidum hemagglutination assay (TPHA) (2) and the microhemagglutination assay (MHA-Tp), and the immunostaining analysis by fluorescent treponemal antibody adsorption test (FTA-ABS) (3). Although the FTA-ABS is tedious to perform and timeconsuming, it is considered to be the most sensitive and specific of all treponemal tests.…”

“…Although the FTA-ABS is tedious to perform and timeconsuming, it is considered to be the most sensitive and specific of all treponemal tests. The ELISA format has been shown to have an excellent sensitivity, comparable to that of the TPHA (3). Enzyme immunoassays can be used not only for the detection of anti Tp-IgG or anti Tp-IgM antibodies, but also for their simultaneous detection using double conjugates (4).…”