Evaluation of the Cardiac Protection Conferred by Proanthocyanidins in Grape Seeds against Development of Ehrlich Solid Tumors in Mice

Abstract

Examination of the antineoplastic effects of a range of chemical compounds is often undertaken via the transplantable tumor model of Ehrlich solid tumor (EST), which is a simulation of breast cancer. The purpose of this study was to explore how cardiac toxicity, damage, oxidative stress, and changes in the expressions of TNFα and apoptotic P53 triggered by EST could be countered with grape seed proanthocyanidins (GSPE). To that end, 50 female mice were used, with arbitrary and equal distribution into five groups, namely, the control group (G1), GSPE group (G2), EST group (G3), GSPE + EST (G4; cotreatment consisted of mice that received GSPE treatment at the beginning of EST induction over a period of 14 days), and EST + GSPE (G5; posttreatment consisted of mice with EST that received GSPE treatment for 14 days following the 14 days since the induction of EST). By comparison with the control group, the EST group had significantly higher levels of serum lactate dehydrogenase (LDH), creatine phosphokinase (CPK), creatine kinase MB (CK-MB), myoglobin, cardiac TBARS, nitric oxide (NO), total thiol and hydrogen peroxide, cardiac damage, and expression of P53 and TNFα. On the other hand, the EST group had significantly lower levels of cardiac catalase and total antioxidant (TAC) than the control group. Furthermore, better improvement in cardiac toxicity, oxidative stress, damage, apoptosis, and TNFα expressions was displayed by the cotreated (GSPE + EST) group than by the posttreated (EST + GSPE) group. This led to the conclusion that GSPE conferred cardiac protective and antioxidant effects against EST. This finding calls for more investigation on the benefits of grape seeds as adjuvant agents to prevent and treat cardiac toxicity.

The Effect of Vitamin B17 on Cardiomyopathy against Ehrlich Tumor Development in Female Mice

“…Cancer cells elicit free radicals to be shaped in excess, destructive lipids, and theoretically generating lipid peroxidation [19], which may be the cause for the notable upsurge in the levels of cardiac MDA persuaded by the EAC group in this study. These findings are also consistent with the results of Aldubayan [20] who revealed that the EAC cells injected into mice showed a significant decrease in TAC and a significant increase in the level of malondialdehyde in the heart tissues. In addition, the results of this study are in good agreement with the results of Joukar et al [21], who showed that MDA levels in the liver, kidneys, and testes are increased due to the formation of EAC tumors.…”

In vivo biological evaluation of sodium salt of ethyl (E)‐2‐cyano‐3‐(7‐hydroxy‐4‐methyl‐2‐oxoquinoline‐1(2H)‐yl)‐3‐(4‐hydroxyphenyl) acrylate as anticancer agent

“…Our results bid that treatment with the sodium salt of the tested compound displayed a highly considerable diminish in the concentration of urea to 30. These results are consistent with those reported by Aldubayan,48 who reported that Ehrlich ascites carcinoma was related to high levels of cardiac enzymes LDH and CK-MB in the serum, supplying proof that cardiac dysfunction occurred in Ehrlich-bearing mice.…”

Novel prevention insights into depletion of oxidative stress status through regular exercise and grape seed effective substance in heart ischemia rat model

“…In addition, GSE prevented excessive ROS accumulation in heart tissue and might increase antioxidant capacity. Moreover, GSE had scavenging properties that reduced the free radicals (Aldubayan, 2020 ; Belviranlı et al., 2012 ). In addition, our data revealed that exercise training with different intensities (low, moderate, and high) increased the relative expression of Mapk3 .…”

“…Furthermore, Aldubayan indicated that GSE consumption significantly decreased creatine phosphokinase, lactate dehydrogenase, creatine kinase as biochemical cardiac markers. Hence, cardiac injuries were reversed by GSE consumption (Aldubayan, 2020 ).…”