Evaluation of the Cariogenic Potential of the Intense Natural Sweeteners Stevioside and Rebaudioside A

Das, Sudip; Das, Arpita; Murphy, Richard A.; Punwani, Indru; Nasution, Muhammad Parlindungan; Kinghorn, A. Douglas

doi:10.1159/000261469

Cited by 86 publications

(57 citation statements)

References 2 publications

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“…Stevia is extracted from the plant Stevia Rebaudiana and Stevia seed is a white and crystalized powder extracted from Stevia leaves. Stevia does not contain any calories, is 250 to 300 times sweeter than sugar and prevents dental caries (Das AK, et al, (1992). Since some Iranian factories use Stevia as the sweetener there is the possibility that the users of these kinds of soft candy show an unnatural decrease in dmft.…”

Section: Discussionmentioning

confidence: 99%

Dietary Habits and Caries Experience Among 9-year-old School Children

Biria¹,

Amini²,

Babaei³

et al. 2015

JMED

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Research Motivation: Despite improvement of the trends in levels and experiences of dental caries in developed countries, it remains prevalent and is increasing in some developing countries. In this study we assessed the relationship between dietary regimens and dental caries in 9-year-old students in Tehran, Iran. Research Design/Methodology: The sample was selected by using multi-stage sampling from all the public primary schools in Tehran. 560 students were assessed using WHO questionnaires and dental examinations according to WHO criteria by indices of dmft and DMFT. The frequency of nutritional snacks consumed was determined in the following week on each individual. The acquired data were described according to abundance charts and indices of means and standard deviations. A linear regression test also was used. Main findings and implications: The mean dmft and DMFT of the students were 3.93 and 0.79 respectively. The study showed that the use of snacks such as cream-filled biscuits (P=0.030), plain cakes (P=0.048), cookies (P=0.008), caramel cream (P=0.009), milk-based ice creams (P=0.017), hard candies (P=0.003), popcorn (P=0.013), cheesy snacks (P=0.000), and bananas (P=0.002) significantly increased dmft in the study group. Furthermore, the use of snacks such as pistachios (P=0.036), cookies (P=0.03), caramel cream (P=0.048) and sugar-based chewing gums (P=0.008) showed a consequential relationship with the increase in DMFT. According to the results, the variety of the consumed snacks was high among 9-year-old students. The dietary habits including Oranges, Pies and Caramel Cream affected the incidence of the dental caries in both deciduous and permanent teeth.

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Section: Discussionmentioning

confidence: 99%

Dietary Habits and Caries Experience Among 9-year-old School Children

Biria¹,

Amini²,

Babaei³

et al. 2015

JMED

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“…Besides the sweetness of Stevia rebaudiana, there are important biological properties that have been identified, such as anti-cariogenic capacity [5], antineoplastic [6], antihyperglycemic [7], antihypertensive [8] and anti-inflammatory activity [9]. However, one of simplest and most reliable method is HPLC and hence in 2010, the FAO/WHO Joint Expert Committee on Food Additives (JECFA) in the 73rd meeting recommended an HPLC method for determination of steviol glycosides [22].…”

Section: Introductionmentioning

confidence: 99%

Rapid HPLC Method for Determination of Rebaudioside D in Leaves of <i>Stevia rebaudiana</i> Bertoni Grown in the Southeast of México

Aranda-González¹,

Moguel-Ordóñez²,

Betancur‐Ancona

2014

AJAC

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Stevia leaves contain glycosides on which biological activity and sweetening capacity has been reported. Besides the main glycosides-stevioside and rebaudioside A-there are minor glycosides that may contribute to the activity and thus it is important to quantify them. Rebaudioside D is one of the minor glycoside present in S. rebaudiana leaves and there are no reports of a validated method to quantify it. Therefore a simple and sensitive high performance liquid chromatography (HPLC) method was validated for the determination of rebaudioside D in leaves of Stevia rebaudiana B. grown in the southeast of México. HPLC method was performed using a C18 column (250 mm × 4.6 mm, 5 μm) and UV detector set at 210 nm. The mobile phase consisted of 32:68 (v/v) mixture of acetonitrile and sodium phosphate buffer (10 mmol/L, pH 2.6), set to a flow rate of 1.0 ml/min. The calculated parameters were: sensitivity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy and precision. The retention time of rebaudioside D was found to be 3.47 min ± 0.04 (S.D.). The calibration curves were linear over the working range (25 -150 μg/ml), with correlation coefficient ≥0.99 and determination coefficient ≥0.98. The calculated limit of detection (LOD) was 8.53 μg/ml, while the limit of quantitation (LOQ) was 25.85 μg/ml. The percent recoveries of fortified samples were 100% ± 10% and precision relative standard deviation was ≤2.79%. The criteria of validation showed accuracy, linearity, and precision; therefore the method is suitable for quantitative analysis of rebaudioside D in Stevia rebaudiana leaves. Rebaudioside D content (g/100g) in Morita II and Criolla varieties grown in the southeast of Mexico were 0.43 and 0.46, respectively with no significant differences (p > 0.05) between them.

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“…16 Therefore, it is considered to be a safe product for teeth. 6 However, due to the fact that this intense sweetener is ≥ 300 times sweeter than sucrose, it has been used in very small quantities and mixed with other sweeteners or their derivatives in foods. 11 In the present study, the commercial product containing stevioside mixed with the artificial substitutes saccharin and cyclamate showed pH falls almost similar to those with the negative control (water).…”

Section: Discussionmentioning

confidence: 99%

“…Stevioside, a natural sweetener extracted from Stevia rebaudiana, offers particular advantages over other non-caloric sucrose substitutes in being heat-stable, resistant to acid hydrolysis, and non-fermentable. 6 Additionally, a caries-preventive action of stevioside extract can be related Declaration of Interests: The authors certify that they have no commercial or associative interest that represents a conflict of interest in connection with the manuscript.…”

Section: Introductionmentioning

confidence: 99%

Effects of lactose-containing stevioside sweeteners on dental biofilm acidogenicity

et al. 2014

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The aim of this study was to evaluate the effect of a commercial lactose-containing stevioside sweetener on biofilm acidogenicity in vivo. Nine volunteers refrained from brushing their teeth for 3 days in five phases. On the 4th day of each phase, the pH of the biofilm was measured by the "Strip method". Interproximal plaque pH was measured before and up to 60 minutes after a 10 mL mouthrinse for 1 minute with the test solutions: I -sweetener with 93% lactose and 7% stevioside; II -sweetener with 6.8% saccharin, 13.6% cyclamate, and 0.82% stevioside; III -18% sucrose solution (positive control); IV -mineral water (negative control); and V-93% lactose solution. The results revealed that the most pronounced pH fall was found with sucrose (positive control), followed by the 93% lactose solution, the sweetener with lactose + stevioside, the sweetener with saccharin + cyclamate + stevioside, and finally water (negative control). According to the area under the curve, the two sweeteners containing stevioside were significantly different, and the sweetener with lactose + stevioside was significantly different from water but not from sucrose. The critical pH for dentin demineralization (pH ≤ 6.5) was reached by all volunteers after rinsing with sucrose solution, lactose solution, and the stevioside + lactose sweetener. Analysis of the data suggests that lactose-containing stevioside sweeteners may be cariogenic, especially to dentin.

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Evaluation of the Cariogenic Potential of the Intense Natural Sweeteners Stevioside and Rebaudioside A

Cited by 86 publications

References 2 publications

Dietary Habits and Caries Experience Among 9-year-old School Children

Dietary Habits and Caries Experience Among 9-year-old School Children

Rapid HPLC Method for Determination of Rebaudioside D in Leaves of <i>Stevia rebaudiana</i> Bertoni Grown in the Southeast of México

Effects of lactose-containing stevioside sweeteners on dental biofilm acidogenicity

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Evaluation of the Cariogenic Potential of the Intense Natural Sweeteners Stevioside and Rebaudioside A

Cited by 86 publications

References 2 publications

Dietary Habits and Caries Experience Among 9-year-old School Children

Dietary Habits and Caries Experience Among 9-year-old School Children

Rapid HPLC Method for Determination of Rebaudioside D in Leaves of &lt;i&gt;Stevia rebaudiana&lt;/i&gt; Bertoni Grown in the Southeast of M&#233;xico

Effects of lactose-containing stevioside sweeteners on dental biofilm acidogenicity

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Rapid HPLC Method for Determination of Rebaudioside D in Leaves of <i>Stevia rebaudiana</i> Bertoni Grown in the Southeast of México