Evaluation of the Chicken Crop as a Source of Salmonella Contamination for Broiler Carcasses

Hargis, B. M.; Caldwell, Daniel J.; Brewer, Robert L.; Corrier, D. E.; DeLoach, John R.

doi:10.3382/ps.0741548

Cited by 118 publications

(48 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The primary function of the crop is food storage while the stomach is full. The chicken crop was shown to routinely harbor Salmonella and was touted as an important source of broiler carcass contamination during processing (3,5,11). Birds challenged with Salmonella serovar Enteritidis showed equal levels of colonization of the crop and intestinal tract (23).…”

Section: Discussionmentioning

confidence: 99%

Isolation ofSalmonella entericaSerovar Enteritidis from Houseflies (Musca domestica) Found in Rooms ContainingSalmonellaSerovar Enteritidis-Challenged Hens

Holt

Geden

Moore

et al. 2007

Appl Environ Microbiol

109

View full text Add to dashboard Cite

Houseflies (Musca domestica) released into rooms containing hens challenged with Salmonella enterica serovar Enteritidis (Salmonella serovar Enteritidis) rapidly became contaminated with Salmonella serovar Enteritidis. Forty to 50% of the flies were contaminated at 48 h, and the percentage increased to 50 to 70% at 4 and 7 days postexposure and then decreased to 30% at day 15. Initial attempts at recovering surface organisms for culture using an aqueous rinse were largely unsuccessful, while cultures of internal contents readily recovered Salmonella serovar Enteritidis. However, when 0.5% detergent was incorporated into the rinse, high recovery levels of bacteria were observed from both external and internal culture regimens, indicating equal distribution of the organism on and in the fly and a tighter interaction of the organism with the host than previously thought. Salmonella serovar Enteritidis was isolated routinely from the fly gut, on rare occasions from the crop, and never from the salivary gland. Feeding contaminated flies to hens resulted in gut colonization of a third of the birds, but release of contaminated flies in a room containing previously unchallenged hens failed to result in colonization of any of the subject birds. These results indicate that flies exposed to an environment containing Salmonella serovar Enteritidis can become colonized with the organism and might serve as a source for transmission of Salmonella serovar Enteritidis within a flock situation.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation ofSalmonella entericaSerovar Enteritidis from Houseflies (Musca domestica) Found in Rooms ContainingSalmonellaSerovar Enteritidis-Challenged Hens

Holt

Geden

Moore

et al. 2007

Appl Environ Microbiol

109

View full text Add to dashboard Cite

show abstract

“…The presence of Salmonella and Campylobacter spp. in the crop of broilers at slaughter represents a human disease risk because of the higher potential of carcass contamination at slaughter from the crop than caecal rupture Hargis et al, 1995;van Gerwe et al, 2010). This emphasizes the need to reduce Salmonella spp.…”

Section: Bacterial Populations and Role In Digestive Tract Health Andmentioning

confidence: 99%

The role of the crop in poultry production

Classen

Apajalahti

Svihus

et al. 2016

World's Poultry Science Journal

View full text Add to dashboard Cite

The importance of the crop is often underestimated in poultry production. In addition to storing ingested feed, it also can impact nutrient digestion by digesta softening and the initial activity of feed (endogenous and exogenous) and microbial enzymes. The crop represents the first major defence against poultry pathogens and zoonotic organisms with well established adaptive and innate immune function, and a lactobacilli dominated microbiota capable of reducing the passage of these organisms further along the digestive tract. However, the potential to improve bird productivity and health, as well as affect meat and egg safety, are influenced by the nature of the diet, and in particular feed entry and extended presence in the crop. This is required to promote lactobacilli fermentation, the production of lactic acid and other volatile fatty acids, and the lowering of crop pH. Management practices such as meal feeding and the use of lighting programs with extended dark periods encourage crop utilisation. Further, the use of feed additives such as prebiotics and probiotics may enhance crop function, which in turn contributes to well-being of the entire digestive tract. A healthy and functional crop, along with other segments of digestive tract, has increased importance in an era of reduced antibiotic use in poultry feeds.

show abstract

“…In the last 20 years, Salmonella enterica serotype Enteritidis has become the most common cause of food poisoning in the United States (19) and in most European countries (4,20), including Italy (26,27); the most commonly implicated foods are eggs and poultry (11,29,38). Although various control measures have been adopted throughout the food production chain (2,6,32), the microbiological testing of poultry products during production and processing still plays a significant role in preventing food-borne infection.…”

mentioning

confidence: 99%

Evaluation of DNA Extraction Methods for Use in Combination with SYBR Green I Real-Time PCR To Detect Salmonella enterica Serotype Enteritidis in Poultry

Medici¹,

Croci²,

Delibato³

et al. 2003

Appl Environ Microbiol

229

132

View full text Add to dashboard Cite

The objective of this study was to develop a rapid, reproducible, and robust method for detecting Salmonella enterica serotype Enteritidis in poultry samples. First, for the extraction and purification of DNA from the preenrichment culture, four methods (boiling, alkaline lysis, Nucleospin, and Dynabeads DNA Direct System I) were compared. The most effective method was then combined with a real-time PCR method based on the double-stranded DNA binding dye SYBR Green I used with the ABI Prism 7700 system. The specificity of the reaction was determined by the melting temperature (T m ) of the amplicon obtained. The experiments were conducted both on samples of chicken experimentally contaminated with serotype Enteritidis and on commercially available poultry samples, which were also used for comparisons with the standard cultural method (i.e., ISO 6579/2001). The results of comparisons among the four DNA extraction methods showed significant differences except for the results from the boiling and Nucleospin methods (the two methods that produced the lowest threshold cycles). Boiling was selected as the preferred extraction method because it is the simplest and most rapid. This method was then combined with SYBR Green I real-time PCR, using primers SEFA-1 and SEFA-2. The specificity of the reaction was confirmed by the T m , which was consistently specific for the amplicon obtained; the mean peak T m obtained with curves specific for serotype Enteritidis was 82.56 ؎ 0.22°C. The standard curve constructed using the mean threshold cycle and various concentrations of serotype Enteritidis (ranging from 10 3 to 10 8 CFU/ml) showed good linearity (R 2 ‫؍‬ 0.9767) and a sensitivity limit of less than 10 3 CFU/ml. The results of this study demonstrate that the SYBR Green I real-time PCR constitutes an effective and easy-to-perform method for detecting serotype Enteritidis in poultry samples.

show abstract

Evaluation of the Chicken Crop as a Source of Salmonella Contamination for Broiler Carcasses

Cited by 118 publications

References 10 publications

Isolation ofSalmonella entericaSerovar Enteritidis from Houseflies (Musca domestica) Found in Rooms ContainingSalmonellaSerovar Enteritidis-Challenged Hens

Isolation ofSalmonella entericaSerovar Enteritidis from Houseflies (Musca domestica) Found in Rooms ContainingSalmonellaSerovar Enteritidis-Challenged Hens

The role of the crop in poultry production

Evaluation of DNA Extraction Methods for Use in Combination with SYBR Green I Real-Time PCR To Detect Salmonella enterica Serotype Enteritidis in Poultry

Contact Info

Product

Resources

About