Evaluation of the clinical utility of platelet aggregation studies in the long‐term follow‐up of patients with atherosclerotic vascular disease

Ferroni, Patrizia; Gazzaniga, Pier Paolo

doi:10.1002/jcla.1860060503

Cited by 10 publications

(6 citation statements)

References 37 publications

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“…The normal values for MAI index range from 0.2 to 2 (i.e. mean value ± 2 SD) as calculated on the basis of the aggregation patterns obtained on PRP from healthy volunteers (32).…”

Section: In Vitro Aggregation Testsmentioning

confidence: 99%

Platelet Activation and Cytokine Production during Hypothermic Cardiopulmonary Bypass – A Possible Correlation?

et al. 1998

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SummaryCardiopulmonary bypass (CPB) is associated with impaired platelet function and a systemic inflammatory response. The present study was designed to evaluate whether any correlation between platelet activation and inflammatory response during CPB exists. The results obtained from 8 patients undergoing hypothermic CPB for cardiac surgery showed the occurrence of a moderate degree of platelet activation during CPB, demonstrated by an increase of platelet CD62P expression in correlation with an increase of β-thromboglobulin levels, with a concomitant decrease of in vitro platelet response. Plasma IL-1β levels significantly increased during CPB, with a peak between 1 and 4 h after CPB. Similarly, IL-6 levels were elevated 30 min from CPB starting, peaked at 4 h, and remained elevated after 24 h. A direct correlation was found between plasma IL-1β and IL-6 levels. A significant correlation between plasma IL-1β and β-thromboglobulin levels was also found. In turn, plasma β-thromboglobulin levels correlated with CD62P expression on activated platelets. An inverse correlation was found between in vitro platelet aggregation and plasma IL-1β or IL-6 levels. From the present results it may be speculated that platelet activation during CPB may contribute, through the release of IL-1β, to activation of endothelial cells and subsequent release of other cytokines with chemotactic and pro-inflammatory properties, thus playing an important role in the inflammatory response associated with CPB.

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“…The normal values for MAI index range from 0.2 to 2 (i.e. mean value ± 2 SD) as calculated on the basis of the aggregation patterns obtained on PRP from healthy volunteers (32).…”

Section: In Vitro Aggregation Testsmentioning

confidence: 99%

Platelet Activation and Cytokine Production during Hypothermic Cardiopulmonary Bypass – A Possible Correlation?

et al. 1998

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“…In vitro platelet aggregation tests are commonly used in the diagnosis of either genetic (1,2j or acquired (3,4) qualitative platelet disorders. Na citrate is the anticoagulant of choice in platelet aggregation studies.…”

Section: Introductionmentioning

confidence: 99%

Acid citrate dextrose (acd) formula a as a new anticoagulant in the measurement of in vitro platelet aggregation

Pignatelli

Pulcinelli

Ciatti

et al. 1995

Clinical Laboratory Analysis

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To evaluate whether the use of ACD Formula A may affect in vitro platelet function, blood samples were obtained from 21 healthy blood donors and anticoagulated in ACD (acid-citrate dextrose, NIH Formula A), Na citrate 3.8%, and K3EDTA. Platelet count, mean platelet volume, and in vitro platelet aggregation were evaluated on each sample. No significant difference was observed in platelet count and mean platelet volume among the different samples. Conversely, the ACD treated platelets showed a higher reactivity to the agonists as demonstrated by a significant increase of the maximum percentages of aggregation induced by ADP, epinephrine, and collagen, as well as a significant decrease of secondary aggregation thresholds to ADP and epinephrine. In conclusion, it may be speculated that ACD Formula A is capable of better maintaining the intraplatelet signal transduction mechanisms during PRP preparation, thus improving the overall responsiveness of platelets.

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“…In vitro platelet aggregation was performed in a PACKS‐4 (Helena Laboratories) aggregometer using sili‐conized glass cuvettes at 37 °C under continuous stirring and was evaluated with the Multiparametric Aggregation Index (MAI) in response to ADP and adrenaline according to Ferroni et al 16 (normal range 0.2–2.0). Platelet response to arachidonic acid (0.5 mmol/L) was also analyzed.…”

Section: Methodsmentioning

confidence: 99%

“…In vitro platelet aggregation was performed in a PACKS-4 (Helena Laboratories) aggregometer using siliconized glass cuvettes at 37 "C under continuous stirring and was evaluated with the Multiparametric Aggregation Index (MAI) in response to ADP and adrenaline according to Ferroni et a1. 16 IL-lP IL-6, sP-selectin, and Endothelin-1 Dctcrminations IL-1p and IL-6 levels were analyzed on serum samples, whereas sP-selectin and Endothelin-1 levels were analyzed on plasma. All samples were aliquoted and stored at -80 "C until assayed and measured in duplicate by enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's instructions.…”

Section: Platelet Aggregationmentioning

confidence: 99%

Platelet Hyperactivity in Hypertensive Older Patients Is Controlled by Lowering Blood Pressure

Riondino¹,

Pignatelli²,

Pulcinelli³

et al. 1999

J American Geriatrics Society

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Evaluation of the clinical utility of platelet aggregation studies in the long‐term follow‐up of patients with atherosclerotic vascular disease

Cited by 10 publications

References 37 publications

Platelet Activation and Cytokine Production during Hypothermic Cardiopulmonary Bypass – A Possible Correlation?

Platelet Activation and Cytokine Production during Hypothermic Cardiopulmonary Bypass – A Possible Correlation?

Acid citrate dextrose (acd) formula a as a new anticoagulant in the measurement of in vitro platelet aggregation

Platelet Hyperactivity in Hypertensive Older Patients Is Controlled by Lowering Blood Pressure

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