Evaluation of the cobas 4800 CT/NG Test for detecting Chlamydia trachomatis and Neisseria gonorrhoeae DNA in urogenital swabs and urine specimens

Parra-Sánchez, Manuel; Palomares, J.C.; Bernal, Samuel; González, María Trinidad; Sivianes, Nieves; Pérez, Luis Francisco; Pueyo, Isabel Casasús; Martín-Mazuelos, Estrella

doi:10.1016/j.diagmicrobio.2012.08.004

Cited by 21 publications

(7 citation statements)

References 25 publications

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“…All samples were tested for N. gonorrhoeae with the Cobas 4800 CT/NG test. This assay targets a direct repeat region called DR-9 that is specific for N. gonorrhoeae (19). The Cobas test utilizes amplification using two sets of primers and probes to detect two highly conserved variations within the DR-9 region.…”

Section: Methodsmentioning

confidence: 99%

Men and Women Have Similar Neisseria gonorrhoeae Bacterial Loads: a Comparison of Three Anatomical Sites

et al. 2020

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Background Neisseria gonorrhoeae (NG) is a common bacterial sexually transmitted infection (STI). Currently, there are limited data on the bacterial load in both men and women, and on both genital and extra-genital sites. Therefore, we quantified NG bacterial load in a large population of women, heterosexual men, and men who have sex with men (MSM) at three different anatomical sites. Methods NG-positive samples (n=1265) of STI clinic consultations (n=944) were tested for NG with the Roche Cobas® 4800 system and Cq-values were used as an inversely proportional measure for NG bacterial load after interpolation from a standard curve. Bacterial load was compared between sample material and sex using t-tests. Results The following mean NG loads were observed: urine, 4.5 ±1.0 log10 CFU/mL; vaginal swabs, 4.3 ±1.1 log10 CFU/mL; anorectal swabs (women), 4.0 ±1.2 log10 CFU/mL; anorectal swabs (men), 4.5 ±1.3 log10 CFU/mL; oropharyngeal swabs (women), 2.8 ±0.9 log10 CFU/mL; and oropharyngeal swabs (men), 3.2 ±1.0 log10 CFU/mL. Oropharyngeal swabs had a significantly lower NG load (p<0.001) compared to genital and anorectal samples. Load did not differ between men and women. Conclusions This is the first study that determined NG load in both women and men at three anatomical sites. The substantial NG load at all sample sites suggest that all sites may have transmission potential. However, the oropharyngeal site presents the lowest bacterial load. Men and women have a similar NG load on separate anatomical sites arguing for similar transmission potential and similar clinical relevance.

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Section: Methodsmentioning

confidence: 99%

Men and Women Have Similar Neisseria gonorrhoeae Bacterial Loads: a Comparison of Three Anatomical Sites

et al. 2020

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“…Chlamydia trachomatis and Neisseria gonorrhoeae (NG) DNA in urine specimens were detected with a Roche Cobas 4800 CT/NG Assay (Roche Molecular Systems, Inc.), which had a sensitivity of 96.6% and a specificity of 100% [ 16 ]. Only participants with positive results were notified, then referred to a local STI clinic or general hospital for treatment.…”

Section: Methodsmentioning

confidence: 99%

Prevalence, risk factors, and medical costs of Chlamydia trachomatis infections in Shandong Province, China: a population-based, cross-sectional study

Huai

et al. 2018

BMC Infect Dis

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BackgroundA population-based study of Chlamydia trachomatis (CT) infections is essential in designing a specific control program; however, no large investigation of CT infections among the general population in mainland China has been conducted since 2000. We aimed to determine the prevalence, risk factors, and associated medical costs of CT among residents, 18–49 years of age, in Shandong, China.MethodsFrom May to August 2016, a multistage probability sampling survey involving 8074 individuals was distributed. Data were collected via face-to-face interviews, followed by self-administered questionnaire surveys. First-void urines were collected and tested for CT and Neisseria gonorrhoeae (NG) using nucleic acid amplification.ResultsThe weighted prevalence of CT infection was 2.3% (95% confidence interval [CI], 1.5–3.2) in females and 2.7% (1.6–3.8) in males. Women, 30–34 years of age, had the highest prevalence of CT infections (3.5%, 2.6–4.4), while the highest prevalence of CT infections in males was in those 18–24 years of age (4.3%, 0.0–8.8). Neisseria gonorrhoeae infection had a prevalence of 0.1% (0.0–0.3) in women and 0.03% (0.0–0.1) in men. Risk factors for CT infections among females included being unmarried, divorced, or widowed (odds ratio [OR], 95% CI 3.57, 1.54–8.24) and having two or more lifetime sex partners (3.72, 1.14–12.16). Among males, first intercourse before 20 years of age (1.83, 1.10–3.02) and having two or more lifetime sex partners (1.85, 1.14–3.02) were associated with CT infections. The estimated lifetime cost of CT infections in patients 18–49 years of age in Shandong was 273 million (range, 172–374 million) China Renminbi in 2016.ConclusionsThis study demonstrated a high burden of CT infections among females < 35 years of age and males < 25 years of age in Shandong. Thus, a CT infection control program should focus on this population, as well as others with identified risk factors.Electronic supplementary materialThe online version of this article (10.1186/s12879-018-3432-y) contains supplementary material, which is available to authorized users.

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“…Estos hallazgos reafirman el rol de la conducta sexual en la prevalencia de infección asintomática por C. trachomatis, más que la variable socio-económica o educacional. Estos datos son concordantes con estudios similares realizados (con la misma técnica) en China, Países Bajos y España, los cuales muestran prevalencias significativamente más altas para esta infección entre trabajadoras sexuales, en población con conducta sexual más liberal o en pacientes que permanecen en control en centros para el manejo de ITS [34][35][36][37] . Otra parte del análisis, consistió en comparar para factores de riesgo las pacientes que resultaron con tamizaje positivo versus aquellas que resultaron con tamizaje negativo.…”

Section: Artículo Originalunclassified

La prevalencia actual de infección genital por Chlamydia trachomatis en adolescentes y mujeres jóvenes chilenas asintomáticas justifica la vigilancia periódica

Zamboni

Ralph

García

et al. 2016

Rev. chil. infectol.

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Evaluation of the cobas 4800 CT/NG Test for detecting Chlamydia trachomatis and Neisseria gonorrhoeae DNA in urogenital swabs and urine specimens

Cited by 21 publications

References 25 publications

Men and Women Have Similar Neisseria gonorrhoeae Bacterial Loads: a Comparison of Three Anatomical Sites

Men and Women Have Similar Neisseria gonorrhoeae Bacterial Loads: a Comparison of Three Anatomical Sites

Prevalence, risk factors, and medical costs of Chlamydia trachomatis infections in Shandong Province, China: a population-based, cross-sectional study

La prevalencia actual de infección genital por Chlamydia trachomatis en adolescentes y mujeres jóvenes chilenas asintomáticas justifica la vigilancia periódica

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