Evaluation of the combined effect of antibiotics and essential oils against Campylobacter multidrug resistant strains and their biofilm formation

Baaboua, Aicha El; Maadoudi, Mohamed El; Bouyahya, Abdelhakim; Belmehdi, Omar; Kounnoun, Ayoub; Cheyadmi, Soumaya; Ouzakar, Sanae; Senhaji, Nadia Skali; Abrini, Jamal

doi:10.1016/j.sajb.2022.08.027

Cited by 19 publications

(17 citation statements)

References 110 publications

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“…Indeed, it is interesting to note that efforts have been made to investigate the bioactive characteristics of EOs from various Origanum species. Numerous studies (Belmehdi et al, 2021; El Baaboua et al, 2022; Rosato, Carocci, et al, 2018; Rosato, Maggi, et al, 2018; Salvagno et al, 2020) have documented the combined activity of Origanum EO in combination with antibiotics, but against other bacterial species, such as S. aureus and Campylobacter spp. In this study, the obtained data showed that the concentration of antibiotics was significantly decreased when mixed with EOs.…”

Section: Discussionmentioning

confidence: 99%

“…The plates were pre-incubated for 1 h at 4 C in order to improve the diffusion of EOs before the growth of bacteria on the agar, and then incubated at 37 C for 24 h. The clear zone diameter generated around the wells, including the 6 mm of each well, was measured in order to evaluate the antibacterial activity. If the inhibition zone was 12 mm or smaller, the EOs were considered as inactive (El Baaboua et al, 2022). Every experiment was run in triplicate.…”

Section: Well Diffusion Methodsmentioning

confidence: 99%

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Combined antibacterial effect of Origanum essential oils and antibiotics against multiresistant foodborne Salmonella isolated from poultry and shellfish in Morocco

Zahli,

El Baaboua,

Belmehdi

et al. 2024

Journal of Food Safety

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The present study aims to assess the antibacterial activity of endemic Moroccan Origanum essential oils (Origanum elongatum and Origanum compactum) alone and in association with antibiotics against multidrug resistant foodborne Salmonella. The well diffusion and microdilution procedures were used to evaluate the antibacterial activity. The synergistic interactions between antibiotics and EOs were determined using the checkerboard assay. The results of the antibacterial activity indicated that all of Salmonella serovars isolates that were examined were highly inhibited by EOs. This effect was confirmed by the microdilution technique, where 0.125% was noted as the smallest MIC value. Based on the obtained MBC values, O. elongatum shown a bactericidal action against the tested strains as indicated by the acquired MBC values. While O. compactum showed this effect against different serovars of Salmonella isolates. Origanum EOs could be employed as an alternative to the former protocols against resistant infectious diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Well Diffusion Methodsmentioning

confidence: 99%

Combined antibacterial effect of Origanum essential oils and antibiotics against multiresistant foodborne Salmonella isolated from poultry and shellfish in Morocco

Zahli,

El Baaboua,

Belmehdi

et al. 2024

Journal of Food Safety

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“…The disk-diffusion technique was used to determine the primary screening of the examined EO antibacterial activity in accordance with the previously published procedures [ 70 , 71 ]. In brief, the culture suspension was inoculated by swabbing on Mueller-Hinton Agar medium (Biokar, Beauvais, France).…”

Section: Methodsmentioning

confidence: 99%

Chemical Composition and Antioxidant, Antimicrobial, and Anti-Inflammatory Properties of Origanum compactum Benth Essential Oils from Two Regions: In Vitro and In Vivo Evidence and In Silico Molecular Investigations

et al. 2022

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The purposes of this investigatory study were to determine the chemical composition of the essential oils (EOs) of Origanum compactum from two Moroccan regions (Boulemane and Taounate), as well as the evaluation of their biological effects. Determining EOs’ chemical composition was performed by a gas chromatography–mass spectrophotometer (GC-MS). The antioxidant activity of EOs was evaluated using free radical scavenging ability (DPPH method), fluorescence recovery after photobleaching (FRAP), and lipid peroxidation inhibition assays. The anti-inflammatory effect was assessed in vitro using the 5-lipoxygenase (5-LOX) inhibition test and in vivo using the carrageenan-induced paw edema model. Finally, the antibacterial effect was evaluated against several strains using the disk-diffusion assay and the micro-dilution method. The chemical constituent of O. compactum EO (OCEO) from the Boulemane zone is dominated by carvacrol (45.80%), thymol (18.86%), and α-pinene (13.43%). However, OCEO from the Taounate zone is rich in 3-carene (19.56%), thymol (12.98%), and o-cymene (11.16%). OCEO from Taounate showed higher antioxidant activity than EO from Boulemane. Nevertheless, EO from Boulemane considerably inhibited 5-LOX (IC50 = 0.68 ± 0.02 µg/mL) compared to EO from Taounate (IC50 = 1.33 ± 0.01 µg/mL). A similar result was obtained for tyrosinase inhibition with Boulemane EO and Taounate EO, which gave IC50s of 27.51 ± 0.03 μg/mL and 41.83 ± 0.01 μg/mL, respectively. The in vivo anti-inflammatory test showed promising effects; both EOs inhibit and reduce inflammation in mice. For antibacterial activity, both EOs were found to be significantly active against all strains tested in the disk-diffusion test, but O. compactum EO from the Boulemane region showed the highest activity. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) for O. compactum EO from the Boulemane region ranged from 0.06 to 0.25% (v/v) and from 0.15 to 0.21% (v/v) for O. compactum from the Taounate region. The MBC/MIC index revealed that both EOs exhibited remarkable bactericidal effects.

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“…Some EOs have an action on quorum sensing, used by bacteria to coordinate and ensure communication between them [ 221 , 222 ]. Several studies have investigated the synergy between EOs components [ 223 , 224 ]. A variation between different outcomes was observed according to the test organism, antibiotic and essential oil tested [ 225 ].…”

Section: Essential Oilsmentioning

confidence: 99%

Alternatives Therapeutic Approaches to Conventional Antibiotics: Advantages, Limitations and Potential Application in Medicine

et al. 2022

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Resistance to antimicrobials and particularly multidrug resistance is one of the greatest challenges in the health system nowadays. The continual increase in the rates of antimicrobial resistance worldwide boosted by the ongoing COVID-19 pandemic poses a major public health threat. Different approaches have been employed to minimize the effect of resistance and control this threat, but the question still lingers as to their safety and efficiency. In this context, new anti-infectious approaches against multidrug resistance are being examined. Use of new antibiotics and their combination with new β-lactamase inhibitors, phage therapy, antimicrobial peptides, nanoparticles, and antisense antimicrobial therapeutics are considered as one such promising approach for overcoming bacterial resistance. In this review, we provide insights into these emerging alternative therapies that are currently being evaluated and which may be developed in the future to break the progression of antimicrobial resistance. We focus on their advantages and limitations and potential application in medicine. We further highlight the importance of the combination therapy approach, wherein two or more therapies are used in combination in order to more effectively combat infectious disease and increasing access to quality healthcare. These advances could give an alternate solution to overcome antimicrobial drug resistance. We eventually hope to provide useful information for clinicians who are seeking solutions to the problems caused by antimicrobial resistance.

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Evaluation of the combined effect of antibiotics and essential oils against Campylobacter multidrug resistant strains and their biofilm formation

Cited by 19 publications

References 110 publications

Combined antibacterial effect of Origanum essential oils and antibiotics against multiresistant foodborne Salmonella isolated from poultry and shellfish in Morocco

Combined antibacterial effect of Origanum essential oils and antibiotics against multiresistant foodborne Salmonella isolated from poultry and shellfish in Morocco

Chemical Composition and Antioxidant, Antimicrobial, and Anti-Inflammatory Properties of Origanum compactum Benth Essential Oils from Two Regions: In Vitro and In Vivo Evidence and In Silico Molecular Investigations

Alternatives Therapeutic Approaches to Conventional Antibiotics: Advantages, Limitations and Potential Application in Medicine

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