Evaluation of the composition of continuously-cultivated Arthrospira (Spirulina) platensis using ammonium chloride as nitrogen source

Sassano, Carlos Eduardo Nascimento; Gioielli, Luíz Antônio; Ferreira, Leila Soares; Rodrigues, Maria Rita; Sato, Sunao; Converti, Attílio; Carvalho, João Carlos Monteiro de

doi:10.1016/j.biombioe.2010.07.002

Cited by 88 publications

(52 citation statements)

References 30 publications

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“…Despite the application of these processes on the evaluation of biomass composition, the microorganisms are submitted to many variables during cultivation, especially nutrient concentration, which makes it difficult to match a biomass composition variation to a certain cause. When cultivating by continuous process, the steady-state assures a better relation between operational conditions and biomass composition [10]. Sobczuk and Chisti [11] studied algal cultivation for biomass and biofuels using steady-state mode, in which the effect of dilution rate or temperature on the lipid contents and fatty acids profiles of the fresh water green microalga was evaluated.…”

Section: Introductionmentioning

confidence: 99%

GrowingChlorella vulgarisin Photobioreactor by Continuous Process Using Concentrated Desalination: Effect of Dilution Rate on Biochemical Composition

Matos

Torres

Morioka

et al. 2014

International Journal of Chemical Engineering

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Desalination wastewater, which contains large amount of salt waste, might lead to severely environmental pollution. This study evaluated the effect of dilution rate (0.1 ≤ ≤ 0.3 day −1 ) on microalgal biomass productivity, lipid content, and fatty acid profile under steady-state condition of Chlorella vulgaris supplemented with concentrated desalination. Continuous culture was conducted for 55 days. Results show that the biomass productivity ( ) varied from 57 to 126 mg L −1 d −1 (dry mass) when the dilution rate ranged from 0.1 to 0.3 day −1 . At lowest dilution rate ( = 0.1 day −1 ), the continuous culture regime ensured the highest values of maximum biomass concentration ( = 570 ± 20 mL −1 ) and protein content (52%). Biomass lipid content was an increasing function of . The most abundant fatty acids were the palmitic (25.3 ± 0.6%) at = 0.1 day −1 and the gamma-linolenic acid (23.5 ± 0.1%) at = 0.3 day −1 ones. These fatty acids present 14 to 18 carbons in the carbon chain, being mainly saturated and polyunsaturated, respectively. Overall, the results show that continuous culture is a powerful tool to investigate the cell growth kinetics and physiological behaviors of the algae growing on desalination wastewater.

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Section: Introductionmentioning

confidence: 99%

GrowingChlorella vulgarisin Photobioreactor by Continuous Process Using Concentrated Desalination: Effect of Dilution Rate on Biochemical Composition

Matos

Torres

Morioka

et al. 2014

International Journal of Chemical Engineering

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“…In fact, in run 2, the lower protein content indicated that there was not enough nitrogen for its accumulation in the form of protein. Thus, the cyanobacterium grew and accumulated energy, most likely in the form of carbohydrates (Sassano et al, 2010).…”

Section: Influence Of Independent Variables On the Growth Of A Platementioning

confidence: 99%

GROWTH AND COMPOSITION OF Arthrospira (Spirulina) platensis IN A TUBULAR PHOTOBIOREACTOR USING AMMONIUM NITRATE AS THE NITROGEN SOURCE IN A FED-BATCH PROCESS

Cruz-Martínez

Jesus

Matsudo

et al. 2015

Braz. J. Chem. Eng.

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-NH 4 NO 3 simultaneously provides a readily assimilable nitrogen source (ammonia) and a reserve of nitrogen (nitrate), allowing for an increase in Arthrospira platensis biomass production while reducing the cost of the cultivation medium. In this study, a 2 2 plus star central composite experimental design combined with response surface methodology was employed to analyze the influence of light intensity (I) and the total amount of added NH 4 NO 3 (M t ) on a bench-scale tubular photobioreactor for fed-batch cultures. The maximum cell concentration (X m ), cell productivity (P X ) and biomass yield on nitrogen (Y X/N ) were evaluated, as were the protein and lipid contents. Under optimized conditions (I = 148 µmol·photons·m -2 ·s -1 and M t = 9.7 mM NH 4 NO 3 ), X m = 4710 ±34.4 mg·L -1 , P X = 478.9 ±3.8 mg·L -1 ·d -1 and Y X/N = 15.87 ±0.13 mg·mg -1 were obtained. The best conditions for protein content in the biomass (63.2%) were not the same as those that maximized cell growth (I = 180 µmol·photons·m -2 ·s -1 and M t = 22.5 mM NH 4 NO 3 ). Based on these results, it is possible to conclude that ammonium nitrate is an interesting alternate nitrogen source for the cultivation of A. platensis in a fed-batch process and could be used for other photosynthetic microorganisms.

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“…Highest protein contents of 699.3 and 686.3 mg/g (on the dry weight basis of biomass) were observed for Spirulina collected from tanks A and B, respectively, after 72-h interval. Sassano et al (2010) reported the increase in the protein content of cyanobacterial biomass to some extent with the increasing nitrogen content. Wu and Pond (1981) observed a protein content of 60.1 and 71.8 % for Spirulina maxima grown on fermented cattle and poultry manure, respectively.…”

Section: Nitrogen Removal and Protein Content Of Spirulinamentioning

confidence: 99%

Sugar mill effluent treatment using Spirulina for recycling of water, saving energy and producing protein

Deshmane

Darandale

Nimbalkar

et al. 2015

Int. J. Environ. Sci. Technol.

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Water security may be regarded as a first step toward achieving food and energy security. Efficient use of fresh water resources and recycling of wastewater after proper treatment are viewed as tools to achieve water sustainability. Sugar industry can have good potential to treat and reuse its effluents. This potential is not realized by prevailing effluent treatment technologies because of high capital and operation cost of treatment process. More upon, these technologies require substantial amount of energy (electricity) as well as chemicals and labors. We have therefore focused on the development of a technology that would help to overcome these limitations. The algaSpirulina-was our choice to (1) treat the effluent and (2) use the sugar mill effluent as its growth medium. Experiments using Spirulina at secondary treatment stage showed 91 % reduction in chemical oxygen demand in 108-h treatment time. Further, biochemical analysis of Spirulina harvested from the sugar mill effluent treatment tanks revealed that the harvested biomass has high protein levels. Spirulina is well known for its usage as a protein supplement and therefore can be used as an additional source of revenue generation.

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Evaluation of the composition of continuously-cultivated Arthrospira (Spirulina) platensis using ammonium chloride as nitrogen source

Cited by 88 publications

References 30 publications

GrowingChlorella vulgarisin Photobioreactor by Continuous Process Using Concentrated Desalination: Effect of Dilution Rate on Biochemical Composition

GrowingChlorella vulgarisin Photobioreactor by Continuous Process Using Concentrated Desalination: Effect of Dilution Rate on Biochemical Composition

GROWTH AND COMPOSITION OF Arthrospira (Spirulina) platensis IN A TUBULAR PHOTOBIOREACTOR USING AMMONIUM NITRATE AS THE NITROGEN SOURCE IN A FED-BATCH PROCESS

Sugar mill effluent treatment using Spirulina for recycling of water, saving energy and producing protein

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