Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds

Babich, Olga; Сухих, Станислав; Pungin, Artem; Astahova, Lidiya; Chupakhin, Evgeny; Belovа, Daria; Prosekov, Alexander; Иванова, Светлана

doi:10.3390/plants10050915

Cited by 9 publications

(4 citation statements)

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“…Individual BAS samples were obtained by separating the Cotinus coggygria extract on a glass chromatographic column by preparative HPLC using a Shimadzu LC-20AD chromatograph (Shimadzu, Kyoto, Japan) and a high-pressure steel column with a sorbent size of 2.5 μm, a diameter of 2.5 mm, and a length of 250 mm [ 22 ]. Elution conditions: flow rate 1 mL/min; eluent water: methanol: 0.1% trifluoroacetic acid with a linear gradient from 40% to 90% methanol in 20 min; detection at a wavelength of 254 nm.…”

Section: Methodsmentioning

confidence: 99%

Study of the Biologically Active Properties of Medicinal Plant Cotinus coggygria

Сухих

Носкова

Pungin

et al. 2021

Plants

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The results of the studies have shown that to obtain an extract of a complex of biologically active substances of Cotinus coggygria, ethyl alcohol (mass fraction of alcohol 70%) with a hydromodule of 1:5 should be used, and the extraction should be carried out for 60 min at a temperature of 60 °C. The investigated plant extracts with the complex of bioactive substances from the Cotinus coggygria leaves and flowers are safe from the point of view of the content of heavy metals, pesticides, aflatoxin B1, radionuclides, as well as pathogenic and opportunistic microorganisms. It has been established that the Cotinus coggygria extract contains rutin, hyperoside, ferulic acid, quercetin, kaempferol, disulphuretin, sulphurein, sulphurein, gallic acid, methyl gallate, pentagalloyl glucose, 3,3′,4′,5,6,7-hexahydroxyflavonone, 3,3′,4′,5,5′,7-hexahydroxyflavonone, 3-O-α-L-rhamnofuranoside, 3,3′,4′,5,5′,7-hexahydroxyflavulium(1+), 7-O-β-D glucopyranoside, and 3,3′,4′,7-tetrahydroxyflavonone. The tested extracts have anticancer, antigenotoxic, and antimicrobial (against E. coli, S. aureus, P. vulgaris, C. albicans, L. mesenteroides) properties. The high antioxidant status of the tested extracts was established; the antioxidant activity of the samples was 145.09 mg AA/g (AA—ascorbic acid).

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Section: Methodsmentioning

confidence: 99%

Study of the Biologically Active Properties of Medicinal Plant Cotinus coggygria

Сухих

Носкова

Pungin

et al. 2021

Plants

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“…In addition, some active ingredients and secondary compounds have successfully been produced by application of tissue culture approach using intact plants ( Figure 4 ). The examples are as listed: phenolic molecules, including apigenin, p-coumaric acid, genistein, luteolin, rutin hydrate, trans-ferulic acid, salicylic acid and naringenin from Coryphantha macromeris ( Karakas and Bozat, 2020 ); medicinally vital phenolic and flavonoid compounds, including apigenin, caffeic acid, catechin, gallic acid, hederagenin, myricetin, kaempherol, isorhamnetin, nahagenin, ursolic acid, betulinic acid from Fagonia indica ( Khan et al., 2019 ); p-coumaric acid, hesperidin, cafeic acid, rosmarinic acid from Rosmarinus officinalis ( Coskun et al., 2019 ); phenolics, including gallic acid, chlorogenic acid, caffeic acid, rutin, myricetin, quercetin, vanillic acid, luteolin and iso-rhamnetin, from Lycium barbarum ( Karakas, 2020 ); gingerol, shogaol, and zingerone from Zingiber officinale ( Arijanti and Suryaningsih, 2019 ); indole alkaloids, including echitamine, acetylechitamine, tubotaiwine and picrinine from Alstonia scholaris ( Jeet et al., 2020 ); crocin from Crocus sativus ( Moradi et al., 2018 ); anticancer alkaloids (vincristine and vinblastine) from Catharanthus roseus ( Mekky et al., 2018 ); phenylethanoid (salidroside, tyrosol), phenylpropanoid (rosavin and rosarin) and phenolic acids (p-coumaric acid, gallic acid, and cinnamic acid) from Rhodiola imbricata ( Rattan et al., 2020 ); eugenol and ursolic acid from Ocimum tenuiflorum ( Sharan et al., 2018 ); bioactive compounds, including 1,2-benzenedicarboxylic acid (phthalic acid), 3,7,11,15-tetramethyl-2-hexadecen-1-ol, 2-hexadecen-1-ol-3,7,11,15-tetrametil, hexadecanoic acid methyl ester (methyl palmitate), n-hexadecanoic acid (palmitic acid), 9,12-octadecadienoic acid methyl ester, 9,12,15-octadecatrienoic acid methyl ester, phytol, octadecanoic acid methyl ester (methyl stearate), 9,12,15-octadecatrienoic acid (linolenic acid) and squalene from Mucuna pruriens ( Sweetlin and Daniel, 2020 ); several different metabolics, including acetamide, propanoic acid, α-thujene, linalool, 5-hydroxymethylfurfural, β-maaliene, epidolichodial, calarene, seychellene, α-curcumene, eremophilene, α-vatirenene, valencene, α-cadinol, ledol, meso-erythritol, α-gurjunene, viridiflorol, (-)-globulol, spirojatamol, dodecanoic acid, patchouli alcohol, jatamansone, xylitol, aristolone, protocatechuic acid, mannose, hexadecanoic acid, p-coumaric acid, talose, α-D-mannopyranose, α-D-galactopyranoside, D-mannitol, myo-inositol, -D-glucopyranoside, D-(+)-trehalose, D-(+)-cellobiose, melibiose, vitamin E, β-sitosterol from Nardostachys jatamansi ( Bose et al., 2019 ); identified 11 organic acids, 16 phenolic acids, 8 flavonoids, and 17 metabolites of different classes from Coryphantha macromeris ( Cabanas-Garcia et al., 2021 ); phenolic compounds (ferulic acid, isoquercitrin, rutin, quercetin, quercetin-7-O-glucoside and luteolin) from Hyssopus officinalis ( Babich et al., 2021 ) and phenylethanoids and st...…”

Section: Tissue Culture-based Biotechnological Approaches For Obtaini...mentioning

confidence: 99%

Production of secondary metabolites using tissue culture-based biotechnological applications

et al. 2023

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Plants are the sources of many bioactive secondary metabolites which are present in plant organs including leaves, stems, roots, and flowers. Although they provide advantages to the plants in many cases, they are not necessary for metabolisms related to growth, development, and reproduction. They are specific to plant species and are precursor substances, which can be modified for generations of various compounds in different plant species. Secondary metabolites are used in many industries, including dye, food processing and cosmetic industries, and in agricultural control as well as being used as pharmaceutical raw materials by humans. For this reason, the demand is high; therefore, they are needed to be obtained in large volumes and the large productions can be achieved using biotechnological methods in addition to production, being done with classical methods. For this, plant biotechnology can be put in action through using different methods. The most important of these methods include tissue culture and gene transfer. The genetically modified plants are agriculturally more productive and are commercially more effective and are valuable tools for industrial and medical purposes as well as being the sources of many secondary metabolites of therapeutic importance. With plant tissue culture applications, which are also the first step in obtaining transgenic plants with having desirable characteristics, it is possible to produce specific secondary metabolites in large-scale through using whole plants or using specific tissues of these plants in laboratory conditions. Currently, many studies are going on this subject, and some of them receiving attention are found to be taken place in plant biotechnology and having promising applications. In this work, particularly benefits of secondary metabolites, and their productions through tissue culture-based biotechnological applications are discussed using literature with presence of current studies.

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“…Root segments were transferred in the laminar air flow hood into 250 mL Erlenmeyer flasks, filled with 100 mL of MS liquid medium supplemented with 5.4 µM α-naphthaleneacetic acid (NAA), 0.5 µM kinetin, and 30 g/L sucrose (pH: 5.8). It has been reported that the combined application NAA + kinetin helps in the production of saponins, flavonoids, and polyphenolic compounds [24][25][26]. The liquid root cultures were placed on a continuous rotary shaker (120 rpm) in a growth chamber (24 h dark, 22 ± 1 • C).…”

Section: Plant Materials and In Vitro Culture Conditionsmentioning

confidence: 99%

Species-Specific Secondary Metabolites from Primula veris subsp. veris Obtained In Vitro Adventitious Root Cultures: An Alternative for Sustainable Production

et al. 2023

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Primula veris subsp. veris L. is a perennial herbaceous and medicinal plant species the roots and flowers of which are a source of valuable pharmaceutical raw materials. The plant tissues are used to produce expectorant and diuretic drugs due to their high content of triterpene saponins and phenolic glycosides. Underground roots of P. veris can be obtained only through a destructive process during the plant’s harvesting. In the present study, an in vitro adventitious root production protocol was developed as an alternative way of production, focused on four species-specific secondary metabolites. Root explants were cultured in Murashing & Skoog liquid medium supplemented with 5.4 μM α-naphthaleneacetic acid, 0.5 μM kinetin, L-proline 100 mg/L, and 30 g/L sucrose, in the dark and under agitation. The effect of temperature (10, 15 and 22 °C) on biomass production was investigated. The content of two flavonoid compounds (primeverin and primulaverin), and two main triterpene saponins (primulic acid I and II) were determined after 60 days of culture and compared with 1.5-year-old soil-grown plants. The accumulated content (mg/g DW) of bioactive compounds of in vitro adventitious roots cultured under 22 °C was significantly higher than the other two temperatures of the study, being 9.71 mg/g DW in primulaverin, 0.09 mg/g DW in primeverin, 6.09 mg/g DW in primulic acid I, and 0.51 mg/g DW in primulic acid II. Compared to the soil-grown roots (10.23 mg/g DW primulaverin, 0.28 mg/g DW primeverin, 17.01 mg/g DW primulic acid I, 0.09 mg/g DW primulic acid II), the in vitro grown roots at 22 °C exhibited a 5.67-fold higher content in primulic acid II. However, primulic acid I and primeverin content were approximately three-fold higher in soil-grown roots, while primulaverin content were at similar levels for both in vitro at 22 °C and soil-grown roots. From our results, tissue culture of P. veris subsp. veris could serve not only for propagation but also for production of species-specific secondary metabolites such as primulic acid II through adventitious root cultures. This would therefore limit the uncontrolled collection of this plant from its natural environment and provide natural products free from pesticides in a sustainable way.

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Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds

Cited by 9 publications

References 34 publications

Study of the Biologically Active Properties of Medicinal Plant Cotinus coggygria

Study of the Biologically Active Properties of Medicinal Plant Cotinus coggygria

Production of secondary metabolites using tissue culture-based biotechnological applications

Species-Specific Secondary Metabolites from Primula veris subsp. veris Obtained In Vitro Adventitious Root Cultures: An Alternative for Sustainable Production

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