Evaluation of the conditions of carotenoids production in a synthetic medium by Sporidiobolus salmonicolor (CBS 2636) in a bioreactor

Abstract

This work studied the cultivation conditions for the production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) in a bioreactor. A Plackett-Burman design was used for the screening of the most important factors, followed by a complete second order design, to maximise the concentration of total carotenoids. The maximum concentration of 3425.9 lg L )1 of total carotenoids was obtained in a medium containing 80 g L )1 glucose, 15 g L )1 peptone and 5 g L )1 malt extract, with an aeration rate 1.5 vvm, 180 r.p.m., 25°C and an initial pH of 4.0. Fermentation kinetics showed that the maximum concentration of total carotenoids was reached after 90 h of fermentation. Carotenoid bio-production was partially associated with cell growth. The specific carotenoid production (Y P ⁄ X ) was 238 lg carotenoids ⁄ g cells, whereas Y P ⁄ S (substrate to product yield) was 41.3 lg g )1 . The specific growth rate (l x ) was 0.045 h )1 . The highest cell and total carotenoid productivity were 0.19 g L )1 h )1 and 56.9 lg L )1 h )1 , respectively.

Microbial Conversion of Waste Glycerol from Biodiesel Production into Value-Added Products

“…Yeast species Sporobolomyces ruberrimus CBS 2636 has recently been investigated for its potential to produce carotenoid using industrial glycerol from the soap manufacturing process as one of the co-substrates [14,66]. The maximum concentration that could be achieved was 3425.9 µg/L with a carotenoid productivity of 56.9 µg/L/h (41.9 µg/g glycerol).…”

Optimization of Carotenoid Production by Paracoccus beibuensis Isolated from Lonar Crater

“…The data suggest that maximum growth and pigment production was obtained at shaking condition with 120rpm. Bacteria can improve the transfer of substrate and oxygen in aerobic condition [19], similar studies were carried out by the Manish Bhat et al; [2] for production of an orange pigment where optimum shaking condition was found at 120 rpm. …”

Biotechnological Production of Carotenoids Using Low Cost-Substrates Is Influenced by Cultivation Parameters: A Review

“…The condensation of the two molecules of GGPP leads to the formation of phytoene, the first 40-carbon carotenoid; in turn, these are desaturated to form phytofluene, neurosporene and lycopene [41,42]. Because lycopene is a trans compound, isomerization of the first or second double bond of phytoene occurs in the same stage as the desaturation reaction [43].…”

“…In the carotenoid pathway in yeasts, γ-carotene appears to be the critical point in the ramification because it acts as the precursor of β-carotene and torulene. In turn, hydroxylation and oxidation of torulene lead to the formation of torularhodin [42].…”

“…For mutant R. acheniorum isolated from milk whey, the maximum optimized production of β-carotene (262.12 µg/mL) was reported at 23 • C [88]. However, R. rubra did not show significant difference at temperatures within 20-30 • C for both production of carotenoids and biomass growth [90], while inoculation temperature at 30 • C provided maximum cell growth for R. diobovatum [89], and the highest production of carotenoid was found at 25 • C for Sporidiobolus salmonicolor [42].…”