Evaluation of the Copan ESwab Transport System for Viability of Pathogenic Fungi by Use of a Modification of Clinical and Laboratory Standards Institute Document M40-A2

Gandhi, B. Kiran; Summerbell, Richard C.; Mazzulli, Tony

doi:10.1128/jcm.01481-17

Cited by 8 publications

(5 citation statements)

References 5 publications

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“…Aside from bacteria, the ESwab also supports the growth of fungi. 6 Because this is a novel use of the ESwab, the clinical laboratory may offer some resistance, as they do when asked to process a blood culture bottle for a vitrectomy specimen. Furthermore, although becoming more popular, not all laboratories may be familiar with receiving specimens collected in ESwabs.…”

Section: Discussionmentioning

confidence: 99%

A Novel Vitreous Culture Technique

Khong

Joondeph

2022

RETINAL Cases &Amp; Brief Reports

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Background/Purpose: This is the first case series of a novel method of collecting and transporting a vitreous specimen after a vitreous tap and injection for infectious endophthalmitis.Methods: Case series.Results: Four consecutive cases of presumed infectious endophthalmitis underwent an in-office vitreous tap and antibiotic injection. The vitreous specimen was injected into the ESwab liquid-based collection and transport system for microbiology samples. Three of the four cases had a positive culture. Conclusion:The ESwab is a simple and efficacious method of collecting a vitreous specimen, increasing the probability of a positive culture, particularly when there is a delay between specimen collection and delivery to the laboratory for processing.

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Section: Discussionmentioning

confidence: 99%

A Novel Vitreous Culture Technique

Khong

Joondeph

2022

RETINAL Cases &Amp; Brief Reports

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“…Escherichia coli and Enterococcus faecalis exhibited resistance to repeated cycles of freezing (–80°C) and thawing in Copan-ESwab tubes, and fewer freeze-thaw cycles yielded better preservation of bacterial viability ( Saliba et al., 2020 ). Copan-ESwab could also effectively maintain the viability of filamentous fungi for at least 48 h ( Gandhi et al., 2018 ). In the present study, Copan-ESwab tubes preserved Candida , the main pathogen causing urogenital tract inflammation in women.…”

Section: Discussionmentioning

confidence: 99%

Copan Walk Away Specimen Processor (WASP) Automated System for Pathogen Detection in Female Reproductive Tract Specimens

Gao

Chen

Peng

et al. 2021

Front. Cell. Infect. Microbiol.

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ObjectiveAutomation is increasingly being applied in clinical laboratories; however, preanalytical processing for microbiology tests and screening is still largely performed using manual methods owing to the complex procedures involved. To promote automation of clinical microbiology laboratories, it is important to assess the performance of automated systems for different specimen types separately. Therefore, the aim of this study was to explore the potential clinical application of the Copan Walk Away Specimen Processor (WASP) automated preanalytical microbiology processing system in the detection of pathogens in female reproductive tract specimens and its feasibility in optimizing diagnostic procedures.MethodsFemale reproductive tract specimens collected from pregnant women at their first obstetric check-up were inoculated into culture media using the Copan WASP automated specimen processing system and were also cultured using a conventional manual inoculation method. After 48 h of culture, the growth of colonies was observed, and the types of bacteria, number of colonies, and efficiency in isolating single colonies were compared between the automated and manual groups. The specimens collected from the WASP system using the Copan-ESwab sample collection tubes were further analyzed for the presence of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Ureaplasmaurealyticum (UU) via fluorescence quantitative polymerase chain reaction (qPCR) and an immunochromatographic assay to investigate the feasibility of this method in optimizing detection of these common pathogens of the female reproductive tract.ResultsCompared with the manual culture method, the Copan WASP microbiology automation system detected fewer bacterial types (P<0.001) and bacterial colonies (P<0.001) but had a higher detection rate of single colonies (P<0.001). There was no significant difference in the detection rates of common pathogens encountered in clinical obstetrics and gynecology, including group B Streptococcus (GBS) (P=0.575) and Candida (P=0.917), between the two methods. Specimens collected in the Copan-ESwab tubes could be used for screening of GBS and CT via fluorescence-based qPCR but not with immunochromatography. However, UU and NG were not detected in any sample with either method; thus, further validation is required to determine the feasibility of the Copan system for screening these pathogens.ConclusionThe Copan WASP microbiology automation system could facilitate the optimization of diagnostic procedures for detecting common pathogens of the female reproductive system, thereby reducing associated costs.

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“…including the yeast C. auris , which is of particular interest to clinicians due to misdiagnosis, link to nosocomial infections and drug resistance of this pathogen. While the recent study by Scansen et al [ 16 ] and Gandhi et al [ 17 ] demonstrated recovery of several Candida spp. and other pathogenic fungi using other commercial STS based on the M40-A2 protocol, they did not investigate recovery of C. auris .…”

Section: Discussionmentioning

confidence: 99%

Laboratory evaluation of the Sigma Transwab® transport system for the recovery of Candida species using the Clinical and Laboratory Standards Institute (CLSI) document M40-A2

Elcocks

Adukwu

2020

Eur J Clin Microbiol Infect Dis

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Rising healthcare complications due to fungal infections increase the importance of efficient specimen collection and maintenance systems for correct identification and diagnosis. The CLSI M40-A2 protocol provides guidelines for laboratories assessing quality of medical transport devices, including swab transport systems (STS). This study assessed the efficiency of the Sigma Transwab® foam and flock swab in recovering and maintaining viability of different Candida spp. including C. auris, in different test conditions. Both swab types recovered and maintained viability of all Candida spp. with greater CFU at room temperature after incubation (24 and 48 h) in comparison with swabs maintained at 4 °C.

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Evaluation of the Copan ESwab Transport System for Viability of Pathogenic Fungi by Use of a Modification of Clinical and Laboratory Standards Institute Document M40-A2

Cited by 8 publications

References 5 publications

A Novel Vitreous Culture Technique

A Novel Vitreous Culture Technique

Copan Walk Away Specimen Processor (WASP) Automated System for Pathogen Detection in Female Reproductive Tract Specimens

Laboratory evaluation of the Sigma Transwab® transport system for the recovery of Candida species using the Clinical and Laboratory Standards Institute (CLSI) document M40-A2

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